The dominant frequency of the advertisement call differed significantly between the two environments (E = -358.4, df = 33, p = 0.048), so that males from the sites in the natural areas (without road noise) showed higher values of the dominant frequency (Figure 5). No other acoustic parameter showed a significant difference between the environments in relation to the presence of traffic or situation (with or without chorus) (Table 2). Additionally, we found a significant positive relationship between the intensity of the calls and the snout-vent length (E = 3.302, df = 33, p = 0.003) (Table 2), that is, the larger the individual the higher the signal intensity (Figure 6). Considering the set of acoustic parameters, there was no influence of the environment or the situation on the acoustic behavior of P. luteolus (F = 0.005, df = 36, p = 0.942).