Correlation analysis
To assess the possible relationship between abnormal ALFF values and
corneal chemical sensitivity, we conducted Pearson’s correlation
analyses of the ALFF values and eye blinking in the 30s after capsaicine
or menthol application (significance level: P< 0.05).
Insular cortex administration
Cyclotraxin-B (HY-P1178, MCE,
New Jersey, USA) was dissolved
in physiological saline and injected into insular cortex using a dual
channel microinjection pump (SP200, Shenzhen Huayang Biotechnology Co.,
Ltd). Infusion cannulas (RWD Life Science Co., Ltd) were placed in the
target insular cortex (50 nl, bregma 0.14 mm; lateral ±3.65mm; ventral
-4.0mm) and cemented to the skull using dental cement (Lang Dental
Manufacturing). After surgery, dummy cannulas were inserted, and caps
were screwed to keep the guide cannula from becoming occluded. Mice were
allowed 7 days to recover.