Results
Excision of
extraorbital glands induced
chemical
and mechanical corneal hypersensitivity and anxiety-like behavior
Dry eye model was established through extraorbital lacrimal gland
removal. Palpebral closure responses to ocular application of capsaicine
and menthol within 30 s were significantly increased from 14 days and
lasted up to 28 days (P<0.0001,
Fig. 1a-b) compared to the Sham group. The corneal mechanical threshold
measured by the proportion of blink responses to von Frey filaments
0.008 g, and 0.02 g (Fig. 1 c, d) was 50% and 60% lower in the DE
group. The open field test was then applied. There was no significant
difference in total traveled distance between groups, while the time in
the center area was significantly reduced in DE (P< 0.001,
Fig. 1f, g). In general, excision of extraorbital glands induces
chemical and mechanical corneal hypersensitivity and anxiety-like
behavior.