Open field test
The open field chamber was made of resin plastic (40cm × 40cm× 35cm), and a 20 cm × 20 cm center square was marked. After acclimation to test room for 60 minutes, mice were allowed 15 min to freely explore the open field. Then, mice were placed in the center of the chamber, behavior was monitored for 5 min with an overhead video-tracking system and analyzed by video tracking system (Smart 3.0, Spanish).