MRI examination
MRI experiments were performed with an 11.7T small animal system (Bruker BioSpec 117/16) with a 6-mm 4-channel surface array coil. Mice were anesthetized and positioned in the scanner with 0.5% oxygen and 1.5% isoflurane. Temperature was maintained at 36.0 ± 1.0 °C, and blood oxygen saturation values were maintained at 96–99%. Standard adjustments included the calibration of the reference frequency power and the shim gradients using MapShim (Paravision v6.1). First, T2-weighted images (T2WI) were acquired as the anatomical reference using a spin echo sequence8. For rs-fMRI acquisition, standard GE-EPI was applied. The acquisition parameters and data preprocessing were as follows: field of view = 18 × 18 cm, matrix = 90 × 90, repetition time = 2000 ms, echo time = 12.31 ms, number of slices = 45, slice thickness = 0.3 mm, volumes = 240, and acquisition time = 8 min. The whole brain was covered.