FIGUE 2
ALFF value comparison in various brain regions. In comparison with the sham group, DE group had significantly increased ALFF in S2, GU, VISC, AUDp, AuV, AlD, AlP, TeA, PERI, Ect, OLF, HIP, RHP, CTXsp, STR (left), while significantly decreased ALFF in the Mop, Mos, SSp-ul, SSp-m and OLF (right), HIP (right), RHP (right), CTXsp (right), SSp-n (right), ACAd (right), PL (right). The Numbers at the top and bottom of the color bar represent the t value of the statistical result. Besides, the red presents the enhancement ALFF signal and the blue shows the decrease ALFF signal. ALFF, amplitude of low-frequency fluctuation; S2, supplemental somatosensory area; GU, gustatory system; VISC, visceral area; AuD, secondary auditory cx, dorsal; AlD, agranular insular cortex, dorsal; AlP, agranular insular cortex, posterior; TeA, temporal association areas; PERI, perirhinal area; Ect, Ectorhinal cortex; OLF, olfactory areas; HIP, hippocampal region; RHP, retrohippocampal region; CTX, cortical subplate; STR, striatum; Mop, primary motor area; Mos, secondary motor area; SSp,primary somatosensory area; ACAd, anterior cingulate area, dorsal part; PL,prelimbic area.
FIGURE 3
C-Fos immunoreactivity in insular cortex and AuV, TeA, Ect brain section of Sham and DE mice. a,d Anatomical mouse brain atlas. Structural elements of the pain matrix are displayed in red frame. b-c, e-f, g-h, j-k C-Fos immunoreactivity of Sham and DE mice at d28. b, e Scale bar = 200 μm. c, f Scale bar = 50 μm. g-h, j-k Scale bar = 100 μm i The number of c-Fos positive neurons per section was significantly greater in DE mice compared with sham mice in insular cortex (i, P=0.0097, n = 3 per group). l, The number of c-Fos positive neurons per section was also significantly greater in DE mice compared with sham mice in AuV, TeA and Ect (l, P=0.0008, n = 4 per group). ***P < 0.001 relative to the sham group. Results are expressed as the mean ±SEM at d28 post-surgery. AuV, secondary auditory cortex, ventral area; Tea, temporal association areas; Ect, Ectorhinal cortex; DE, dry eye.