Statistical analysis
The statistical analyses were performed using GraphPad Prism statistical software. We performed power analysis to calculate the number of experimental mice. Data are reported as mean ± standard error of the mean (SEM). Differences between sham and surgery groups were assessed through two-sample t-tests performed in Graphpad Prism 9.0. Values of P< 0.05 was considered to indicate significance. To explore the ALFF between dry eye disease mice and the sham, the standardized ALFF data were analyzed with two independent samples t-tests. The correction standard was determined by Gaussian random field (GRF), with P<0.001 at the voxel level and P<0.05 at the cluster level, indicating a significant difference.