Mouse dry eye model
The mice were anesthetized and the skin under the ear was disinfected with iodine. We use ophthalmic scissors to make 5-7mm incisions under the ears. The exorbital lacrimal gland was isolated gently and removed. The skin was sutured with 5–0 non-absorbable suture silk thread (ETHICON, China). The same procedure was done to the Sham group without extraorbital lacrimal gland removal.