FIGURE 6
Single-dose injection of cyclotraxin-B into the insular cortex inhibited
chronic corneal pain induced chemical and mechanical corneal
hypersensitivity. (a) Diagram and timeline schema of the experimental
design. Grey arrows mark local infusion of cyclotraxin-B or saline into
the insular cortex. Bilateral extraorbital glands were excised on day 0.
Insular cortex cannula was inserted on day 21 after surgery. Dark grey
circles mark behavior test including chemical and mechanical corneal
sensitivity test. (b-e) Effect of treatment with single dose injection
of BDNF antagonist cyclotraxin-B induces a statistically significant
antinociceptive effect in chemical and mechanical corneal sensitivity.
The DE+Cyclotraxin-B group represents that of dry eye mice receiving an
intra-insular cortical microinjection of cyclotraxin-B (10 µg/µL, 0.05
µL per side; MCE). (n=8, ***P < 0.001and ****P <
0.0001 versus DE+Saline group). ELG, extraorbital gland; CTB,
cyclotraxin-B; DE, dry eye.