FIGURE 1
Evaluation of chemical and mechanical corneal sensitivity and anxiety-like behavior of Sham and DE animals. a, b Chemical corneal sensitivity was evaluated using a drop of capsaicine (100 μM) (a) and methol (100 μM) recording the palpebral closure frequency for 30s. Sham, n =8 mice; DE, n = 8 mice. c, d Mechanical corneal sensitivity tested by recording proportion of blink responses to application of von Frey filaments 0.008 g (c) and 0.02 g (d) to the cornea in Sham and DE mice. e Representative activity tracking OFT in Sham and DE mice. f,g Time in center region (f) and total distance traveled (g) during OFT. Sham, n = 8 mice; DE, n = 8 mice. ***P< 0.001, ****P < 0.0001, ns, not significant. DE, dry eye; OFT, open field test.