FIGURE 6
Single-dose injection of cyclotraxin-B into the insular cortex inhibited chronic corneal pain induced chemical and mechanical corneal hypersensitivity. (a) Diagram and timeline schema of the experimental design. Grey arrows mark local infusion of cyclotraxin-B or saline into the insular cortex. Bilateral extraorbital glands were excised on day 0. Insular cortex cannula was inserted on day 21 after surgery. Dark grey circles mark behavior test including chemical and mechanical corneal sensitivity test. (b-e) Effect of treatment with single dose injection of BDNF antagonist cyclotraxin-B induces a statistically significant antinociceptive effect in chemical and mechanical corneal sensitivity. The DE+Cyclotraxin-B group represents that of dry eye mice receiving an intra-insular cortical microinjection of cyclotraxin-B (10 µg/µL, 0.05 µL per side; MCE). (n=8, ***P < 0.001and ****P < 0.0001 versus DE+Saline group). ELG, extraorbital gland; CTB, cyclotraxin-B; DE, dry eye.