The levels of BDNF and inflammatory cytokines expression
increased in insular cortex, AuV, TeA, and Ect regions in chronic
corneal pain mice
To clarify underlying central mechanism, we further investigated the
expression of BDNF and inflammatory cytokines from the insular cortex
(AID, AIP, GI, DI) and AuV, TeA, Ect brain tissue. RT-qPCR showed mRNA
levels of BDNF, TNF-α, IL-6, and IL-1β (Figure 4a-4c, Figure 4e) in
insular cortex of DE group statistically increased compared with the
Sham group (P<0.05), and level of IL-10 (Figure 4d) was significantly
reduced (P<0.05). Meanwhile, as shown in Figure S1, expression levels
of BDNF, IL-6, and IL-1β, except TNF-α (Figure 4 f, h, j), were
significantly higher. In contrast, the expression level of IL-10 (Figure
4i) was significantly lower in AuV, TeA, Ect of DE group (P<0.05).