FIGURE 5
Correlation analysis of ALFF values of S2, AID, AIP, AuV, TeA, Ect and eye blinking times after chemical application.
Local injection of cyclotraxin-B in insular cortex blocked the corneal hypersensitivity and inhibited upregulation of inflammatory cytokines in chronic corneal pain mice
The behavior paradigm and timeline are shown in Figure 6a and Figure 7a. Single-dose (Figure 6b-e) and continuous (Figure 7f-i) local infusion of cyclotraxin-B (10 µg/µL, 0.05 µL per side; MCE) into the insular cortex of DED mice had a significant effect on corneal hypersensitivity (p < 0.0001), suggesting a promising therapeutical effect on chronic corneal pain. The expression of pro-inflammatory cytokines, including IL-6, IL-1β, and TNF-α, was significantly reduced after consecutive injection (5 days, 10 µg/µL, 0.05 µL per side) of cyclotraxin-B in the insular cortex (P<0.05, Figure 7b-e). However, there was no effect on DE-related anxiety-like behavior compared to saline infusion controls (Figure 7j-l).