FIGURE 5
Correlation analysis of ALFF values of S2, AID, AIP, AuV, TeA, Ect and
eye blinking times after chemical application.
Local injection of cyclotraxin-B in insular cortex
blocked the corneal
hypersensitivity and inhibited upregulation of inflammatory cytokines in
chronic corneal pain mice
The
behavior paradigm and timeline are shown in Figure 6a and Figure 7a.
Single-dose (Figure 6b-e) and continuous (Figure 7f-i) local infusion of
cyclotraxin-B (10 µg/µL, 0.05 µL per side; MCE) into the insular cortex
of DED mice had a significant effect on corneal hypersensitivity (p
< 0.0001), suggesting a promising
therapeutical effect on chronic corneal pain. The
expression of pro-inflammatory cytokines, including IL-6, IL-1β, and
TNF-α, was significantly reduced after consecutive
injection (5 days, 10 µg/µL, 0.05 µL per side) of cyclotraxin-B in the
insular cortex (P<0.05, Figure 7b-e). However, there was no effect on
DE-related anxiety-like behavior compared to saline infusion controls
(Figure 7j-l).