Experimental approach:
In vivo, we explored dynamic characteristics of renal tissue in
hyperuricemia-related CKD. By incorporating mRNA and protein sequencing
data, we analyzed gene expression profile, hub genes and potential
pathways responsible for disease development, which was further
confirmed using qPCR, western blotting, and immunofluorescent stainings.
In vitro, we validated bioinformatic findings under different conditions
of macrophages with interventions corresponding to core nodes in
pathway.