Animal experimental design
Seven-week male mice (C57BL/6J, 22-25g) were purchased from Dossy Experimental Animal Co., Ltd (Chengdu, China). These mice were maintained in the controlled environment for 1 week (temperature at 20 ± 2 ℃, humidity at 50–60%, and 12-hour light/dark cycle) and provided with food and water ad libitum. To study dynamic characteristic of uric acid-induced kidney injury, we randomly divided mice into 4 groups: day 0, day 7, day 14, and day 21 groups (n=6 per group). The mice in group day 7, 14 and 21 were administered with a mixture of adenine (0.16 g/kg) and potassium oxonate (2.4 g/kg) by oral gavage once daily, as previously described by our team(Pan et al., 2019; Ren et al., 2021). At day 0, 7, 14, and 21, each group anaesthetized with pentobarbital sodium (50 mg/kg, i.p) and sacrificed. Terminal blood were immediately centrifuged and stored at -80 ℃ after collection. Kidney was divided and samples were processed and kept in 10% phosphate buffered formalin, 2.5% glutaraldehyde, or liquid nitrogen depending on purpose, and followed by quick delivery to storage. Animal research was performed in strict accordance with the Animal Research: Reporting of In Vivo Experiments guidelines(Percie du Sert et al., 2020). This study received approval granted by Animal Care and Use Ethics Committee of Sichuan University (Approval No. 20220303052).