Statistical analysis
Results are presented as mean ± standard deviation based on independent
experiments. Sample sizes estimation per group were calculated using
“resource equation” and confirmed or confirmed by post hoc analyses in
G*power 3 software, as previous described. Student’s t-test was used for
two-group comparison. One-way ANOVA and further Tukey test were used for
multiple comparisons. A two-sided p value less than 0.05 was considered
statistically significant and reported.