Progressive renal function decline and kidney fibrosis in
hyperuricemia-related CKD
From day 0 to day 21, we set four time points and investigated dynamic
characteristics of blood, urine, and kidney samples in
hyperuricemia-related CKD mice (Figure 1A ). Serum uric acid
kept rising and reached peak at day 21. Similar trend to uric acid was
observed in renal function measurements. Serum creatinine and urea went
up quickly from day 0 to day 7, kept stable at high level at day 14, and
then sharply increased to three and four times of baseline level
respectively. Urine albumin-to-creatinine (UACR) rose rapidly and became
5-fold level at day 7 compared to day 0, and then slowly declined but
still at high level at day 14 and 21 (Figure 1B ). Histologic
changes in PAS-staining sections indicated progressive tubular and
glomerular injuries. Trichrome-staining sections presented the
increasing deposition of collagen in tubular interstitial and glomerular
mesangial compartments (Figure 1C ). We further observed
progressive inflammatory cell recruitment indicated by chemokine MCP-1,
pro-inflammatory cytokine TNF-α, IL-6, IL-1β (Figure 1D ), as
well as fibrotic marker fibronectin, collagen-I, α-SMA (Figure
1E ).