Cell culture and treatments
The mouse macrophage cell line (Raw 264.7) and mouse kidney proximal tubular cell line (TCMK1) were obtained from Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences. Raw 264.7 (RRID: CVCL_0493) and TCMK1 (RRID: CVCL_2772) cells were cultured in RPMI 1640 and DMEM/F12 media (both were supplemented with 10% FBS), respectively. To investigate whether tubular cell is the essential mediator of effect from uric acid on macrophage, we co-cultured Raw 264.7 and TCMK1 together by putting transwell inserts carrying TCMK1 to 6-well plates with Raw 264.7. Cells were exposure to uric acid at concentration of 800 μM, as previously reported(Ren et al., 2021). Based on different intervention purposed, before adding uric acid, cells were treated with Itgam siRNA (Genepharma Co., Ltd., Suzhou, China), FAK inhibitor (PF562271, Selleck Chemicals LLC, USA), and Akt inhibitor (MK-2206 2HCl, Selleck Chemicals LLC, USA) as instructed.