Transcriptomics
The mRNA was sequenced by Majorbio Bio-Pharm Technology Co. Ltd. (Shanghai, China). In brief, total RNA was extracted from the tissue using TRIzol® Reagent (Invitrogen, CA), followed by sample integrity, quality, and purity accordingly. Double-stranded cDNA was synthesized using a SuperScript double-stranded cDNA synthesis kit (Invitrogen, CA) with random hexamer primers (Illumina). After size selection and quantification, mRNA sequencing was performed using Illumina HiSeq xten/NovaSeq 6000 sequencer (2 × 150 bp read length). A dataset of clean reads aligned to reference genome was established for bioinformatic analysis.