Figure Legends
Figure 1 . Collagen deposition within skeletal muscle measured
via Picrosirius Red staining during the progression of cancer cachexia
in the A) plantaris (female) and B) tibialis anterior muscle (male).N of 4-5 per group. Different letters denote statistical
significance at an alpha set a P ≤ 0.05. 1wk, 2wk, 3wk, 4wk, denotes 1,
2, 3, and 4 weeks of cancer progression, respectively; LT and HT denote
low tumor and high tumor, respectively; PBS, phosphate-buffered saline.
Different letters denote statistical significance at an alpha set a P ≤
0.05.
Figure 2 . Collagen 1 and 3 mRNA abundance during the
progression of cancer cachexia in females (left) and males (right).
(A-B) Collagen 1 and 3 mRNA abundance in female tibialis anterior
muscle. (C) Collagen 3:1 mRNA abundance female tibialis anterior muscle.
(D-E) Collagen I and III mRNA abundance in male tibialis anterior
muscle. (F) Collagen 3:1 mRNA abundance male tibialis anterior muscle.
For females, N of 10-14 per group were used. For males, Nof 7-8 per group were used. 1wk, 2wk,
3wk, 4wk, denotes 1, 2, 3, and 4 weeks of cancer progression,
respectively; LT and HT denote low tumor and high tumor, respectively;
PBS, phosphate-buffered saline.
Different letters denote statistical
significance at an alpha set a P ≤ 0.05.
Figure 3. mRNA abundance of markers of extracellular matrix
remodeling and turnover during the progression of cancer cachexia in
females (left) and males (right). (A-B) MMP2 and MMP9 mRNA abundance in
female tibialis anterior muscle. (C-D) MMP2 and MMP9 mRNA abundance in
male tibialis anterior muscle. For females, N of 10-14 per group
were used. For males, N of 7-8 per group were used. 1wk, 2wk,
3wk, 4wk, denotes 1, 2, 3, and 4 weeks of cancer progression,
respectively; LT and HT denote low tumor and high tumor, respectively;
PBS, phosphate-buffered saline. Different letters denote statistical
significance at an alpha set a P ≤ 0.05.
Figure 4 . mRNA abundance of the
TGF-β/SMAD signaling pathway
components during the progression of cancer cachexia. (A-C) Relative
mRNA abundance of TGF-β1, SMAD 2/3 in female tibialis anterior muscle.
(D-F) Relative mRNA abundance of TGF-β1, SMAD 2/3 in male tibialis
anterior muscle. For females, N of 10-14 per group were used. For
males, N of 7-8 per group were used. 1wk, 2wk, 3wk, 4wk, denotes
1, 2, 3, and 4 weeks of cancer progression, respectively; LT and HT
denote low tumor and high tumor, respectively; PBS, phosphate-buffered
saline. Different letters denote statistical significance at an alpha
set a P ≤ 0.05.
Figure 5. Effect of LCM treated media on C2C12 myotube size.
(A) Representative images of C2C12 myotubes in media treated with
vehicle or LCM. (B) Myotube diameter on C2C12 myotubes treated with
either Vehicle (Veh) or LLC conditioned media (LCM). Different letters
denote statistical significance at an alpha set a P ≤ 0.05.
Figure 6 . Effect of LCM treated media and co-culture on ECM
regulation in C2C12 myotubes. (A-C) mRNA abundance of collagen1, 3, and
the collagen 3 to 1 ratio. (D-F). mRNA abundance of ECM remodeling and
turnover markers MMP2, 9, and TIMP-1. Different letters denote
statistical significance at an alpha set a P ≤ 0.05.
Figure 7. Effect of LCM treated media and co-culture on SMAD
mRNA abundance in C2C12 myotubes or 3T3 fibroblasts treated with vehicle
(Veh) or LLC conditioned media (LCM) treated. Different letters denote
statistical significance at an alpha set a P ≤ 0.05.