Methods
Female and male C57BL6/J mice were injected with PBS or Lewis Lung
Carcinoma (LLC) at 8‐week of age and tumors were allowed to develop for
1, 2, 3, or 4 weeks. 3wk and 4wk female tumor‐bearing mice displayed a
dichotomy in tumor growth and were reassigned to high tumor (HT) and low
tumor (LT) groups. In vitro analyses were also performed on
co-cultured C2C12 and 3T3 exposed to LLC conditioned media.
Immunohistochemistry and quantitative PCR analysis were used to
investigate fibrosis and fibrosis related signaling in skeletal muscle.