3.1 Sampling Site

Samples of water-hyacinth were identified and collected from Ibiteinye Integrated farms ltd, Elelenwo, Port Harcourt, Rivers state, Nigeria located between latitude 4.85730 N and longitude 7.07570 E. While cow dung was obtained from an abattoir at marina junction, Eket, Akwa Ibom State situated at latitude 4.6275531 N and longitude 7.9323988E. These were then transported to the Department of Marine Biology laboratory, Akwa Ibom State University, Akwa Ibom State where the experiment was conducted.