2.7.3 Investigation of the effect of Gemcitabine
The impact of perfusing 5 µM GEM in the system for 1, 3 and 7 days on HCT-116 cells was assessed in terms of metabolic activity by Alamar blue (AB) assay according to supplier’s instructions. After each timepoint, a specific cell culture medium containing 20% v/v AB was added to a recipient covering the entire device. After 4 h of incubation, the fluorescence signal was read at 590 nm (excitation wavelength 530 nm) using a microplate reader (FL 600, Bio-Tek Instruments). After each AB determination, cells were washed and a fresh culture medium was added to continue the culture. Medium with 20% v/v AB was used as a blank.