2.6.1 Mechanical properties
The 6%, 12%, and 14% eSF hydrogels storage and loss moduli were
evaluated using an oscillatory model in a rheometer (Kinexus Prot,
Malvern). For each measurement, eSF solution (1 mL) was mixed with HRP
(100 µL) and H2O2 (65 µL), which was
poured (100 µL) into circular silicone molds of 8 mm in diameter at
37°C. After crosslinking, the eSF hydrogels were located into the plate
of the rheometer to study. The measurements were conducted at 37°C (n =
3). First stress sweeps (0.1 Hz) were performed to determine LVR for all
the tested conditions. Frequency sweeps (0.01–1 Hz) were then performed
within the LVR. The values of the elastic modulus (G′) presented in
Table 1 were obtained at a frequency of 0.1 Hz.