2.4 Cell seeding
HCT-116 cancer cells were encapsulated in eSF hydrogel at 10 × 106 cells mL-1 and allowed to crosslink within the PDMS mold in the incubator. After peeling, the eSF hydrogel was bonded to the flat eSF layer, and the microchannel was coated with 10% Matrigel solution for 30 min at 37°C. HCoMECs (10 × 106 cells.mL-1) were manually injected in the microchannel and left to adhere for 6 h in the incubator. Then, the cells were exposed to a perfusion flow of 8 µL per h for the entire experiment duration (1 and 3 days and 7).