2.7.2 Live/Dead assay
The viability of HCT-116 cells encapsulated in the eSF hydrogel
microfluidic chip was assessed by live/dead assay. For this, at
endpoints, a portion randomly cut from the device was washed with PBS
(Sigma-Aldrich) and incubated for 40 min with calceĆn AM (1 mM; live
cells in green) and ethidium-homodimer (6 mM; dead cells in red).
Microfluidic chips were then imaged using an inverted SP8 confocal
microscope (Leica, Germany) using automated acquisition for Z-stack and
multi-color channel.