Diversity of fungal isolates revealed by cultivation
Metabarcoding using the relatively short reads often does not allow accurate identification at species level. Thus, to determine the exact identity of ASVs identified by metabarcoding, we cultured and identified the dominant fungi, (bacteria were identified elsewhere by Peral-Aranega et al. in press and are deposited in GenBank with accession numbers OP935778-OP935900). In total, we isolated 108 fungal strains belonging to 12 species (8 yeasts, 2 filamentous fungi). The most frequently isolated species were Ogataea ramenticola , Meyerozyma guilliermondii , Wickerhamomyces bisporus , Nakazawaea ambrosiae , and Ophiostoma bicolor (Figure 2, Supplementary Table 1).