RNA metabarcode
The total number of reads processed was 848,460,028. The final number of
contigs obtained was 457,282 totaling 301,840,624 bp with N50 of 711 bp.
The number of ORFs identified reached 411,387. Results obtained from
metatranscriptome analysis were in accordance with the results from DNA
metabarcode analysis. In the Bacteria domain, the phylum
Gammaproteobacteria dominated (48% of reads, Fig. 4); however, not so
strongly as in DNA metabarcode analysis and other classes, mainly
Betaproteobacteria (21% of reads) and Actinomycetia (6.6% of reads),
were also abundant (Fig. 4). The most abundant bacterial genera revealed
by DNA metabarcode were also detected in RNA metabarcode, especially
genus Erwinia , which belongs among the most abundant genera with
almost 5% of reads. Another highly represented genera wereKlebsiella and Enterobacter (both belonging to
Enterobacteriaceae) (Fig. 4).
Although RNA was isolated from the insect’s gut, 91% of eukaryotic
reads belong to Arthropoda. The second most abundant eukaryotic phylum
was Nematoda with 1.9% of reads. Fungal domain took only 0.35% of
reads which almost all belong to Ascomycota. Similarly to DNA
metabarcode analysis, the most abundant fungi in the metatranscriptome
dataset were yeasts from the class Saccharomycetes, followed by
Sordariomycetes (Fig. 4). Other presented classes include Pezizomycetes,
Eurotiomycetes, Leotiomycetes, Lecanoromycetes and Orbilliomycetes.
However, these classes have minor representation.