Diversity of fungal isolates revealed by cultivation
Metabarcoding using the relatively short reads often does not allow
accurate identification at species level. Thus, to determine the exact
identity of ASVs identified by metabarcoding, we cultured and identified
the dominant fungi, (bacteria were identified elsewhere by Peral-Aranega
et al. in press and are deposited in GenBank with accession numbers
OP935778-OP935900). In total, we isolated 108 fungal strains belonging
to 12 species (8 yeasts, 2 filamentous fungi). The most frequently
isolated species were Ogataea ramenticola , Meyerozyma
guilliermondii , Wickerhamomyces bisporus , Nakazawaea
ambrosiae , and Ophiostoma bicolor (Figure 2, Supplementary Table
1).