Supplementary figure 2 The effect of TNF-α and IL-5 on ICOSL
expression of purified eosinophils
(A). The purity of isolated eosinophils verified by Flow cytometry (left
panel) and Wright-Giemsa staining (right panel, x 1000). (B). The
expression level of CD40 and ICOSL on purified eosinophils at 0h
accessed by flow cytometry. (C). The effect of TNF-α and IL-5 on ICOSL
expression of eosinophils isolated from healthy donators. GM-CSF (50
ng/mL) stimulation used as basic condition (medium) for all groups.
Freshly isolated eosinophils were cultured with medium alone, or rhTNF-α
(50 ng/mL), or rhIL-5 (50 ng/mL) or rhTNF-α plus rhIL-5. ICOSL
expression was then detected by Flow cytometry.