Figure 8 The effect of TNF-α and IL-5 on CD40 expression of purified eosinophils
GM-CSF (50 ng/mL) was added as a basic condition (medium) to keep eosinophils culture for all groups. Freshly isolated eosinophils from healthy controls were cultured with medium alone, or rhTNF-α (50 ng/mL), or rhIL-5 (50 ng/mL) or rhTNF-α plus rhIL-5 in the absence or presence of specific p38 inhibitor SB203580 (10 uM) for 24h and 48h. And SB203580 was preincubated with eosinophils for 1h before cytokines stimulation. CD40 expression was subsequently detected by Flow cytometry.