CD40-CD40L and ICOS-ICOSL interactions enhance the activation of
eosinophils from ECRS patients
Since upregulation of CD40 and ICOSL expression on eosinophils in nasal
tissue of CRS patients, we investigated whether CD40-CD40L and
ICOS-ICOSL interactions involved in eosinophils dysfunction. Considering
that ECRS is characterized circulating and histologically high
proportion of eosinophils. To determine this, peripheral blood samples
from 10 ECRS patients were stimulated by rhCD40L (5ug/mL), rhICOS (10
ug/mL), or control IgG (5ug/mL), respectively. Cells were harvested 24h
post-stimulation for flow cytometry.
CD45+C16- cells was defined as
eosinophils and CD69 was an activation marker of eosinophils. The
detailed gating strategy was shown in Fig. 7A. There were notably
upregulation CD69 expression on eosinophils in response to CD40L and
ICOS protein stimulation compared with that to control IgG group (Fig.
7B). These data indicated that the up-regulation of CD40 and ICOSL on
eosinophils mediated their activation in ECRS patients.