Rg1 delayed the aging of NSCs
To explore whether Rg1 could delayed D-gal induced aging of NSCs,we
used CCK-8 assay (Table.3) revealed a significant improvement in the
ability of cells in the D-gal +Rg1 group to proliferate when compared to
cells in the D-gal group,In comparison to cells in the Control group,
the D-gal group had a strong inhibitory effect on cell
proliferation.SA-β-gal (Fig.2 A, B) revealed that whereas the D-gal
group had a higher percentage of senescence neurospheres than the
Control group, the D-gal group had a significantly lower percentage of
senescence neurospheres than the D-gal +Rg1 group(paired t
test,n=3,p<0.05). Trypan blue staining (Fig.2 C) results showed that
the cell survival rate of the D-gal group was significantly lower than
that of the Control group,However, as compared to the D-gal group, the
D-gal +Rg1 group demonstrated a considerable increase in cell
survival(paired t test,n=6,p<0.05). The above results indicated that
Rg1 could delayed the aging of NSCs induced by D-gal.