Measurement of oxidation-related biomarkers
Following treatment, the hippocampus and NSCs were lysed in RIPA Lysis
Buffer (Beyotime, China), centrifuged and the supernatant (containing
protein) was collected, and the protein concentration was measured with
BCA kit(Beyotime Institute of Biotechnology ,China). SOD, CAT, and
MDA(Zhou H et al,2022) activities in hippocampus homogenate and NSCs
were determined by chemical colorimetric method following the
manufacturer’s instructions. 8-OHdG and 4-HNE activity in hippocampal
homogenate and NSCs were determined by ELISA.