Rg1 delayed the aging of NSCs
To explore whether Rg1 could delayed D-gal induced aging of NSCs,we used CCK-8 assay (Table.3) revealed a significant improvement in the ability of cells in the D-gal +Rg1 group to proliferate when compared to cells in the D-gal group,In comparison to cells in the Control group, the D-gal group had a strong inhibitory effect on cell proliferation.SA-β-gal (Fig.2 A, B) revealed that whereas the D-gal group had a higher percentage of senescence neurospheres than the Control group, the D-gal group had a significantly lower percentage of senescence neurospheres than the D-gal +Rg1 group(paired t test,n=3,p<0.05). Trypan blue staining (Fig.2 C) results showed that the cell survival rate of the D-gal group was significantly lower than that of the Control group,However, as compared to the D-gal group, the D-gal +Rg1 group demonstrated a considerable increase in cell survival(paired t test,n=6,p<0.05). The above results indicated that Rg1 could delayed the aging of NSCs induced by D-gal.