Identification of the Nestin protein expressed by NSCs by immunofluorescence
Nestin staining could identify NSCs(Seonghee Jung et al.2020).Under a microscope, it was observed that the NSCs developed into neurospheres (Fig.1A). NSCs were identified using Nestin immunofluorescence staining(Fig.1B), with green fluorescence representing positive NSCs when observed under a fluorescence microscope (×100). The result showed that the real NSCS was extracted