The protective mechanism of Rg1 against oxidative stress damage was mediated by the Keap1-Nrf2/ARE pathway
To determine whether the protective mechanism of Rg1 against oxidative stress injury was mediated by the Keap1-Nrf2/ARE pathway, we determined the expression of proteins associated with the Keap1-Nrf2/ARE pathway (Fig.4 A-D),Nrf2 and HO-1 protein expression level were lower in the D-gal group than in the Control and D-gal +Rg1 group, while Keap1 protein expression level were greater in the D-gal group than in the Control and D-gal +Rg1 group (paired t test,n=3,p<0.05). We also measured the expression of mRNA related to the Keap1-Nrf2/ARE pathway ( Fig.4 E-H),in comparison to the Control group, the expression levels of NQO1,GCLM,GSTM-1 and GCLC mRNA was decreased in the D-gal group, but the expression levels of these mRNA increased in the D-gal +Rg1 group(paired t test, n=5, p<0.05). The above results indicated that the protective mechanism of Rg1 against oxidative stress injury of NSCs was mediated by the Keap1-Nrf2/ARE pathway