Western Blot
The same method was used to obtain the protein of the hippocampus and NSCs and measured the protein concentration. SDS-PAGE was used to separate the proteins, which were then transferred to a PVDF membrane and blocked for 1 hour with TBST and skimmed milk powder. The membranes were incubated using a primary antibody(1:1000) overnight at 4°C, then with a secondary antibody(1:10000) for 1 hour at room temperature. The ECL light-emitting system was used to develop colors, and quantitative analysis was performed using Image Lab software.