Separation, purification, and grouping of NSCs
Whole-brain tissue was obtained from C57BL/6J fetal mice, separated, purified, and culture cells, culture them in serum-free medium to the third generation, NSCs were identified using Nestin immunofluorescence, and randomly divided into four groups. In the Rg1 group, NSCs were treated with PBS and incubated for 24 hours before being treated with Rg1 (20 μg/mL) and incubated for another 24 hours. NSCs were treated with D-gal (10 mg/mL) and cultured for 48 hours in the D-gal group. The NSCs in the D-gal + Rg1 group were treated with D-gal (10 mg/mL) and incubated for 24 hours, and then treated with Rg1 (20 μg/mL) and incubated for 24 hours. The Control group was incubated for 48 hours with the same amount of PBS. Relevant indicators were detected on the second day after Modeling.