Reagents
Ginsenoside Rg1 (RSZD-121106, Purity = 98.6%) was purchased from Jilin Hongjiu Biological Technology Co, Ltd (Jilin, China), dissolved in PBS at a concentration of 20mg/mL, and sterilized by ultrafiltration. OriCell/C57BL/6J mice NSCs complete medium (MUBNF-90011) was obtained from Cyagen Biosciences Inc (Guangzhou, China). D-galactose (SG8010, Purity≥98%) was obtained from Solarbio Science&Technology Co, Ltd (Beijing, China). SOD (A001-3-2), MDA (A003-1-2), CAT (A007-1-1), 4-HNE (H268) ,8-OHdG (H165) and ChAT(A079-1-1) kits were purchased from the Nanjing Jiancheng Bioengineering Institute (Nanjing, China). AChE kit was purchased from Solarbio LIFE SCIENCES. BCA (P0012S), Mouse monoclonal anti-Nestin antibodies (AN203), Trypan blue staining (C0011), and SA-β-gal staining (C0602) kits were purchased from Beyotime Institute of Biotechnology (Nanjing, China).The antibody against P53(#ab26) was purchased from Abcam (United Kingdom), Nrf2 (#12721), Keap1 (#7705), and HO-1 (#86806) were purchased from Cell Signaling Technology Inc (Boston, USA). Trizol reagent (B511311) was purchased from Sangon Biotech (Shanghai, China). cDNA Reverse Transcription Kit (RR047A) and TBGreen® Premix Ex Taq™ II (RR820A) were obtained from Takara Biotechnology (Dalian, China).