Identification of the Nestin protein expressed by NSCs by
immunofluorescence
Nestin staining could identify
NSCs(Seonghee
Jung et al.2020).Under a microscope, it was observed that the NSCs
developed into neurospheres (Fig.1A). NSCs were identified using Nestin
immunofluorescence staining(Fig.1B), with green fluorescence
representing positive NSCs when observed under a fluorescence microscope
(×100). The result showed that the real NSCS was extracted