Measurement of oxidation-related biomarkers
Following treatment, the hippocampus and NSCs were lysed in RIPA Lysis Buffer (Beyotime, China), centrifuged and the supernatant (containing protein) was collected, and the protein concentration was measured with BCA kit(Beyotime Institute of Biotechnology ,China). SOD, CAT, and MDA(Zhou H et al,2022) activities in hippocampus homogenate and NSCs were determined by chemical colorimetric method following the manufacturer’s instructions. 8-OHdG and 4-HNE activity in hippocampal homogenate and NSCs were determined by ELISA.