H&E staining
Pentobarbital was used to anesthetize the mice, and the entire brain tissue was swiftly taken via decapitation. For 24 hours, the specimen was fixed in 4% paraformaldehyde, dehydrated in ethanol, made transparent with p-xylene, embedded in paraffin, coronal sectioned, and stained with hematoxylin and eosin (HE) ( Yang L et al,2017). Under an optical microscope, the histology of the hippocampus CA1 region was examined.