Western Blot
The same method was used to obtain the protein of the hippocampus and
NSCs and measured the protein concentration. SDS-PAGE was used to
separate the proteins, which were then transferred to a PVDF membrane
and blocked for 1 hour with TBST and skimmed milk powder. The membranes
were incubated using a primary antibody(1:1000) overnight at 4°C, then
with a secondary antibody(1:10000) for 1 hour at room temperature. The
ECL light-emitting system was used to develop colors, and quantitative
analysis was performed using Image Lab software.