The protective mechanism of Rg1 against oxidative stress damage was mediated by the Keap1-Nrf2/ARE pathway
Same as in vitro experiment, We measured the expression of proteins related to the Keap1-Nrf2/ARE pathway in the hippocampus of mice(Fig.7 A-D),we found that Nrf2 and HO-1 protein expression levels were significantly lower in the D-gal group than in the Control and D-gal + Rg1 group, Keap1 protein expression levels were significantly higher in the D-gal group than in the Control and D-gal + Rg1 group (paired t test,n=3,p<0.05). We also measured the expression of mRNA related to the Keap1-Nrf2/ARE pathway ( Fig.7 E-H), compared with D-gal group, the mRNA expression levels of NQO1, GCLM, GSTM-1 and GCLC decreased significantly, but the expression levels of D-gal+Rg1 group and Control group increased significantly (paired t-test, n=3, p<0.05). The above results indicated that the protective mechanism of Rg1 against oxidative stress injury in hippocampus was mediated by the Keap1-Nrf2/ARE pathway.