2.5 Protocol 3: Anecdotal information on non-imaged, non-vouchered specimens.
2.5.1 Culturing and morphological data. This protocol is clearly distinguished from any other by the lack of any image data for the specimens. Information about single/bulk-sequenced specimens is, therefore, anecdotal at best. We did not apply this method, but it was used previously by others to generate the published y-larva sequences in NCBI GenBank of “Facetotecta spp.” and H. itoi (Pérez-Losada et al., 2002, 2009; Gallego et al., 2015). These authors used different DNA extraction kits but successfully amplified long and short ribosomal and protein-coding mitochondrial (16S and COX1, respectively) and nuclear (18S, 28S, and H3, respectively) markers.