REFERENCES
1. Zhu H, Das P, Roberson DW, et al. Hospitalizations in children with
preexisting tracheostomy: a national perspective. Laryngoscope .
Feb 2015;125(2):462-8. doi:10.1002/lary.24797
2. Russell CJ, Thurm C, Hall M, Simon TD, Neely MN, Berry JG. Risk
factors for hospitalizations due to bacterial respiratory tract
infections after tracheotomy. Pediatr Pulmonol . Jan 4
2018;doi:10.1002/ppul.23938
3. Funamura JL, Yuen S, Kawai K, et al. Characterizing mortality in
pediatric tracheostomy patients. Laryngoscope . Jul
2017;127(7):1701-1706. doi:10.1002/lary.26361
4. Niederman MS, Ferranti RD, Zeigler A, Merrill WW, Reynolds HY.
Respiratory infection complicating long-term tracheostomy. The
implication of persistent gram-negative tracheobronchial colonization.Chest . Jan 1984;85(1):39-44.
5. Rusakow LS, Guarin M, Wegner CB, Rice TB, Mischler EH. Suspected
respiratory tract infection in the tracheostomized child: the pediatric
pulmonologist’s approach. Chest . Jun 1998;113(6):1549-54.
6. Morrison JM, Hassan A, Kysh L, Dudas RA, Russell CJ. Diagnosis,
management, and outcomes of pediatric tracheostomy-associated
infections: A scoping review. Pediatr Pulmonol . May
2022;57(5):1145-1156. doi:10.1002/ppul.25873
7. Warniment A, Steuart R, Rodean J, et al. Variation in Bacterial
Respiratory Culture Results in Children With Neurologic Impairment.Hospital pediatrics . Nov 2021;11(11):e326-e333.
doi:10.1542/hpeds.2020-005314
8. Bomar L, Brugger SD, Lemon KP. Bacterial microbiota of the nasal
passages across the span of human life. Curr Opin Microbiol . Feb
2018;41:8-14. doi:10.1016/j.mib.2017.10.023
9. Perez-Losada M, Graham RJ, Coquillette M, et al. The temporal
dynamics of the tracheal microbiome in tracheostomised patients with and
without lower respiratory infections. PloS one .
2017;12(8):e0182520. doi:10.1371/journal.pone.0182520
10. Perez-Losada M, Graham RJ, Coquillette M, et al. Tracheal Microbiota
in Patients With a Tracheostomy Before, During and After an Acute
Respiratory Infection. Pediatr Infect Dis J . Nov
2018;37(11):e269-e271. doi:10.1097/inf.0000000000001952
11. Cline JM, Woods CR, Ervin SE, Rubin BK, Kirse DJ. Surveillance
tracheal aspirate cultures do not reliably predict bacteria cultured at
the time of an acute respiratory infection in children with tracheostomy
tubes. Chest . Mar 2012;141(3):625-631. doi:10.1378/chest.10-2539
12. Russell CJ, Mack WJ, Schrager SM, Wu S. Care Variations and Outcomes
for Children Hospitalized With Bacterial Tracheostomy-Associated
Respiratory Infections. Hospital pediatrics . Jan 2017;7(1):16-23.
doi:10.1542/hpeds.2016-0104
13. Feudtner C, Christakis DA, Connell FA. Pediatric deaths attributable
to complex chronic conditions: A population-based study of Washington
state, 1980-1997. Pediatrics . Jul 2000;106(1):205-209.
14. Feudtner C, Feinstein JA, Zhong W, Hall M, Dai D. Pediatric complex
chronic conditions classification system version 2: updated for ICD-10
and complex medical technology dependence and transplantation. BMC
pediatrics . 2014;14:199. doi:10.1186/1471-2431-14-199
15. Russell CJ, Simon TD, Neely MN. Development of Chronic Pseudomonas
aeruginosa-Positive Respiratory Cultures in Children with Tracheostomy.Lung . Dec 2019;197(6):811-817. doi:10.1007/s00408-019-00285-6
16. Team RC. R: A language and environment for statistical computing.MSOR connections . 2014;1
17. Brugger SD, Frei L, Frey PM, Aebi S, Mühlemann K, Hilty M. 16S rRNA
terminal restriction fragment length polymorphism for the
characterization of the nasopharyngeal microbiota. PloS one .
2012;7(12):e52241. doi:10.1371/journal.pone.0052241
18. Bogaert D, Keijser B, Huse S, et al. Variability and diversity of
nasopharyngeal microbiota in children: a metagenomic analysis.PloS one . Feb 28 2011;6(2):e17035.
doi:10.1371/journal.pone.0017035
19. Willson DF, Conaway M, Kelly R, Hendley JO. The lack of specificity
of tracheal aspirates in the diagnosis of pulmonary infection in
intubated children. Pediatric critical care medicine : a journal
of the Society of Critical Care Medicine and the World Federation of
Pediatric Intensive and Critical Care Societies . May
2014;15(4):299-305. doi:10.1097/pcc.0000000000000106
20. Yalamanchi S, Saiman L, Zachariah P. Decision-Making Around Positive
Tracheal Aspirate Cultures: The Role of Neutrophil Semiquantification in
Antibiotic Prescribing. Pediatric critical care medicine : a
journal of the Society of Critical Care Medicine and the World
Federation of Pediatric Intensive and Critical Care Societies . Aug
2019;20(8):e380-e385. doi:10.1097/pcc.0000000000002014
21. Durairaj L, Mohamad Z, Launspach JL, et al. Patterns and density of
early tracheal colonization in intensive care unit patients. J
Crit Care . Mar 2009;24(1):114-21. doi:10.1016/j.jcrc.2008.10.009
22. Ichinohe T, Pang IK, Kumamoto Y, et al. Microbiota regulates immune
defense against respiratory tract influenza A virus infection.Proc Natl Acad Sci U S A . Mar 29 2011;108(13):5354-9.
doi:10.1073/pnas.1019378108
23. Novak T, Hall MW, McDonald DR, et al. RIG-I and TLR4 responses and
adverse outcomes in pediatric influenza-related critical illness.J Allergy Clin Immunol . Jun 2020;145(6):1673-1680.e11.
doi:10.1016/j.jaci.2020.01.040
24. Hasegawa K, Mansbach JM, Ajami NJ, et al. The relationship between
nasopharyngeal CCL5 and microbiota on disease severity among infants
with bronchiolitis. Allergy . Nov 2017;72(11):1796-1800.
doi:10.1111/all.13160
25. Hasegawa K, Mansbach JM, Ajami NJ, et al. Serum cathelicidin,
nasopharyngeal microbiota, and disease severity among infants
hospitalized with bronchiolitis. J Allergy Clin Immunol . Apr
2017;139(4):1383-1386.e6. doi:10.1016/j.jaci.2016.09.037
26. Mansbach JM, Luna PN, Shaw CA, et al. Increased Moraxella and
Streptococcus species abundance after severe bronchiolitis is associated
with recurrent wheezing. J Allergy Clin Immunol . Feb
2020;145(2):518-527.e8. doi:10.1016/j.jaci.2019.10.034
27. Wasserman MG, Graham RJ, Mansbach JM. Airway Bacterial Colonization,
Biofilms and Blooms, and Acute Respiratory Infection. Pediatric
critical care medicine : a journal of the Society of Critical Care
Medicine and the World Federation of Pediatric Intensive and Critical
Care Societies . Jun 30 2022;doi:10.1097/pcc.0000000000003017
28. Espahbodi M, Kallenbach S, Huang CC, Chun RH, McCormick ME.
Significance of positive tracheal cultures in the 30 days following
tracheostomy. Int J Pediatr Otorhinolaryngol . Jul
2020;134:110028. doi:10.1016/j.ijporl.2020.110028
29. Levin D, Tribuzio M, Green-Wrzesinki T, et al. Empiric antibiotics
are justified for infants with respiratory syncytial virus lower
respiratory tract infection presenting with respiratory failure: a
prospective study and evidence review. Pediatric critical care
medicine : a journal of the Society of Critical Care Medicine and the
World Federation of Pediatric Intensive and Critical Care Societies .
May 2010;11(3):390-5. doi:10.1097/PCC.0b013e3181b809c5
30. Harlid R, Andersson G, Frostell CG, Jörbeck HJ, Ortqvist AB.
Respiratory tract colonization and infection in patients with chronic
tracheostomy. A one-year study in patients living at home. Am J
Respir Crit Care Med . Jul 1996;154(1):124-9.
doi:10.1164/ajrccm.154.1.8680667
31. Morar P, Singh V, Makura Z, et al. Oropharyngeal carriage and lower
airway colonisation/infection in 45 tracheotomised children.Thorax . Dec 2002;57(12):1015-20.
32. Prinzi A, Parker SK, Thurm C, Birkholz M, Sick-Samuels A.
Association of Endotracheal Aspirate Culture Variability and Antibiotic
Use in Mechanically Ventilated Pediatric Patients. JAMA Netw
Open . Dec 1 2021;4(12):e2140378. doi:10.1001/jamanetworkopen.2021.40378
33. Willson DF, Hoot M, Khemani R, et al. Pediatric
Ventilator-Associated Infections: The Ventilator-Associated INfection
Study. Pediatric critical care medicine : a journal of the Society
of Critical Care Medicine and the World Federation of Pediatric
Intensive and Critical Care Societies . Jan 2017;18(1):e24-e34.
doi:10.1097/pcc.0000000000001001
34. Jain S, Williams DJ, Arnold SR, et al. Community-Acquired Pneumonia
Requiring Hospitalization among U.S. Children. N Engl J Med . Feb
26 2015;372(9):835-845. doi:10.1056/NEJMoa1405870
35. Hotterbeekx A, Xavier BB, Bielen K, et al. The endotracheal tube
microbiome associated with Pseudomonas aeruginosa or Staphylococcus
epidermidis. Sci Rep . Nov 4 2016;6:36507. doi:10.1038/srep36507
36. Kim SK, Lee JH. Biofilm dispersion in Pseudomonas aeruginosa.J Microbiol . Feb 2016;54(2):71-85. doi:10.1007/s12275-016-5528-7
37. Trevethan R. Sensitivity, Specificity, and Predictive Values:
Foundations, Pliabilities, and Pitfalls in Research and Practice.Front Public Health . 2017;5:307. doi:10.3389/fpubh.2017.00307
38. Tsukahara K, Johnson B, Klimowich K, et al. Comparison of tracheal
aspirate and bronchoalveolar lavage samples in the microbiological
diagnosis of lower respiratory tract infection in pediatric patients.Pediatr Pulmonol . Oct 2022;57(10):2405-2410.
doi:10.1002/ppul.26049
39. Prinzi Andrea M, Parker Sarah K, Curtis Donna J, Ziniel Sonja I,
Turenne Christine Y. The Pediatric Endotracheal Aspirate Culture Survey
(PETACS): Examining Practice Variation across Pediatric Microbiology
Laboratories in the United States. J Clin Microbiol .
59(3):e02232-20. doi:10.1128/JCM.02232-20
40. McCaleb R, Warren RH, Willis D, Maples HD, Bai S, O’Brien CE.
Description of Respiratory Microbiology of Children With Long-Term
Tracheostomies. Respir Care . Apr 2016;61(4):447-52.
doi:10.4187/respcare.03518