2.7 Arabidopsis Plant material and Growth condition
Arabidopsis thaliana (L.) Heynh ecotypes Col-0, homozygous mutant
and over expression (OE) lines used in this study. T-DNA insertion
mutant of rpoC1 (cs835205 ) was obtained from theArabidopsis Biological Resource Centre (ABRC, Ohio State
University, Columbus, OH, USA; https://abrc.osu.edu/). Seed
sterilization and plants growth were performed according to Tiwariet al. , (2021). Homozygosity of rpoC1 mutant confirmed by
polymerase chain reaction (PCR) by using gene-specific right primer with
a T-DNA left border specific primer (pDAP110/ pCSA110) listed in TableS1 . For over-expression of RpoC1 gene alleles from
B1K-09-07 and B1K-50-04 in Col-0 background, coding sequence region
(2046 bps without stop codon) were synthesized at Genewiz
(www.genewiz.com) in pUC-GW-Amp vector. Further all the desired fragment
were cloned in binary vector (pICH86966 Addgene) using Golden Gate
assembly (Plant part: 1000000047 and toolkit: 1000000044, Addgene)
(Figure S3 ).
RESULTS