3.2 Plasmotype effects on the plasticity circadian clock and ChlF traits in reciprocal hybrids
We also included a photosynthetic rhythmicity analysis to the RH under OT and HT using SensyPAM platform (See Methods). The average Fv/Fm was significantly higher under HT (0.93 ±0.01) in comparison to OT (0.92 ±0.01; Figure 1d ) and significantly different for the average NPQlss per se (0.66 ±0.1 vs. 0.43 ±0.08) under OT in comparison to HT (Figure 1e ). We calculated the amplitude and the period of the NPQlss rhythmicity and found that the former was significantly higher under HT (0.03 ±0.01) compared to OT (0.015 ±0.006) (Figure1f ). This is compared to the period, which was significantly higher under OT (24.9 ±2.6 h) compared to HT (23.3 ±1.9 h; Figure1g ). This clock plasticity is similar to the one described for the ASHER (Bdolach et al. , 2019) with acceleration of the rhythmicity under HT. Moreover, these results suggest that under HT, photosynthesis is more efficient than under OT.
In another way of comparing the contribution of the plasmotype variation to the phenotypic variation and plasticity we counted the number of reciprocal hybrids that significantly differ in the life history traits and Chl F traits (Figure 2 ). Note that pairs of RH are sharing similar nucleotype (heterozygous) and differ only in their plasmotype identity therefore significant difference between the two types of non-segregating hybrids point to effect of the plasmotype on the trait. We clustered phenotypic traits measured in the net house as growth or reproductive and traits measured with SensyPAM as the clock or Chl F parameters. The percentage of different pairs of RH was highest for the Fv/Fm under OT (44.8%), and the lowest was zero hybrids for the difference of DTF under AT. If comparing the traits according to our clustering, we could see that the mean number of differing reciprocal hybrids is highest for Chl F (26.3%), second for clock traits (15%), and falling behind for growth and reproductive traits with 5.23% and 3.73%, respectively (Figure 2 ).
Overall, these analyses implicate a significant contribution of the plasmotype diversity to the phenotypic plasticity, and moreover and to more extent, for the plasticity of the photosynthesis and its circadian rhythmicity.