2.4 Statistical analysis
The JMP version 14.0 statistical package (SAS Institute, Cary, NC, USA)
was used for statistical analyses. Student’s t-Tests between treatments,
plasmotypes and alleles were conducted using the ‘Fit Y by X’ function.
A factorial model was employed for the analysis of variance (ANOVA),
using ‘Fit model’, with temperature or plasmotype treatment and allelic
state as fixed effects. In the SensyPAM experiments we measured the F
parameters every 2.5 hr for three days. The Fv/Fm and NPQlss for each
time point where calculated for the circadian clock analysis in the
BioDare (https://biodare2.ed.ac.uk). The Fv/Fm and NPQlss traits per se
are the average of these three days measurements.