2.4 Statistical analysis
The JMP version 14.0 statistical package (SAS Institute, Cary, NC, USA) was used for statistical analyses. Student’s t-Tests between treatments, plasmotypes and alleles were conducted using the ‘Fit Y by X’ function. A factorial model was employed for the analysis of variance (ANOVA), using ‘Fit model’, with temperature or plasmotype treatment and allelic state as fixed effects.