River channels store large volumes of water globally, critically impacting ecological and biogeochemical processes. Despite the importance of river channel storage, there is not yet an observational constraint on this quantity. We introduce a 26-year record of entirely remotely sensed volumetric channel water storage anomaly (VCWS) on 26 major world rivers. We find mainstem VCWS climatology amplitude (VCWSCA) represents an appreciable amount of basin-wide terrestrial water storage variability (median 2.2%, range 0.05-13.8% across world rivers), despite the fact that mainstem rivers themselves represent an average of just 0.2% of basin area. We find that two global river routing schemes coupled with land surface models reasonably approximate VCWSCA (within {plus minus}50%) in only 19.2 % and 23.1 % of rivers considered (by model). These findings demonstrate VCWS is a useful measurement for assessing global hydrological model performance, and for advancing understanding of spatial patterns in global hydrology.