The cohort in the study was divided into three group: fetuses with ultrasonographic anomalies, fetuses with soft markers, and fetuses with no anomalies (normal ultrasonography and maternal age ≥ 35 years). Gestational week was divided into three groups: First trimester (11w-13w+6d), Second trimester (14w-27w+6d), and Third trimester (28w-). pCNV: pathogenic/likely pathogenic copy number variations.