Figure 2. Augmented cholinergic gamma oscillations in B6N and 129 mouse strains. (a) Gamma oscillations were induced by perfusion of 5 µM carbachol and recorded from CA3 and CA1 pyramidal layers in vitro . (b) Representative cholinergic gamma oscillations in the CA3 and CA1. Note the augmented gamma oscillations in the B6N and 129 in comparison to B6J. (c) Representative power spectra of CA3 gamma oscillations (B6J: N=8 mice, n=24 slices; B6N: N=7 mice, n=14 slices; 129, N=7 mice, n=19 slices) from three strains. Note that to reduce the variability of gamma power in vitro , Log10 converted values are used. Summary graphs illustrating increased (d) gamma integrated power (20-80 Hz) and (e) gamma peak power in the B6N and 129 compared to B6J.(f) Summary graph showing a significant increase in gamma peak frequency of 129 in comparison to C57 sub-strains. (g)Representative power spectra of CA1 gamma oscillations from three strains (B6J: N=8 mice, n=18 slices; B6N: N=7 mice, n=13 slices; 129, N=7 mice, n=13 slices). Summary graphs illustrating (h) no significant alterations of integrated power (20-80 Hz) but (i)an enhanced peak power in B6N compared to B6J. (j) Summary graph showing a significant increase in gamma peak frequency of 129.(k) Representative auto-correlogram of CA3 gamma oscillations from three strains. Correlation peak values indicated by the arrow were further used for statistical comparison of local gamma correlations. Summary graphs showing enhanced (l) local CA3-CA3 (B6J: N=8 mice, n=24 slices; B6N: N=7 mice, n=14 slices; 129, N=7 mice, n=19 slices) and (m) CA1-CA1 gamma correlations (B6J: N=8 mice, n=18 slices; B6N: N=7 mice, n=13 slices; 129, N=7 mice, n=13 slices) in B6N strain. (n) No statistical differences were detected for CA3-CA1 cross correlation of gamma oscillations (B6J: N=8 mice, n=18 slices; B6N: N=7 mice, n=13 slices; 129, N=7 mice, n=13 slices). Statistical comparison for (d), (e), (h), (i), (l), (m):one-way-ANOVA followed by posthoc comparison using Fisher LSD Method. Statistical comparison for (f), (j), (n): Kruskal-Wallis One Way Analysis of Variance on Ranks followed by posthoc comparison using Dunn´s Method. Post-hoc statistical differences are indicated via *p<0.05 and **p<0.01. Data are presented ± standard error of mean (SEM). Empty circles represent individual data points.