Figure 2. Augmented cholinergic gamma oscillations in B6N and
129 mouse strains. (a) Gamma oscillations were induced by
perfusion of 5 µM carbachol and recorded from CA3 and CA1 pyramidal
layers in vitro . (b) Representative cholinergic gamma
oscillations in the CA3 and CA1. Note the augmented gamma oscillations
in the B6N and 129 in comparison to B6J. (c) Representative
power spectra of CA3 gamma oscillations (B6J: N=8 mice, n=24 slices;
B6N: N=7 mice, n=14 slices; 129, N=7 mice, n=19 slices) from three
strains. Note that to reduce the variability of gamma power in
vitro , Log10 converted values are used. Summary graphs
illustrating increased (d) gamma integrated power (20-80 Hz)
and (e) gamma peak power in the B6N and 129 compared to B6J.(f) Summary graph showing a significant increase in gamma peak
frequency of 129 in comparison to C57 sub-strains. (g)Representative power spectra of CA1 gamma oscillations from three
strains (B6J: N=8 mice, n=18 slices; B6N: N=7 mice, n=13 slices; 129,
N=7 mice, n=13 slices). Summary graphs illustrating (h) no
significant alterations of integrated power (20-80 Hz) but (i)an enhanced peak power in B6N compared to B6J. (j) Summary
graph showing a significant increase in gamma peak frequency of 129.(k) Representative auto-correlogram of CA3 gamma oscillations
from three strains. Correlation peak values indicated by the arrow were
further used for statistical comparison of local gamma correlations.
Summary graphs showing enhanced (l) local CA3-CA3 (B6J: N=8
mice, n=24 slices; B6N: N=7 mice, n=14 slices; 129, N=7 mice, n=19
slices) and (m) CA1-CA1 gamma correlations (B6J: N=8 mice, n=18
slices; B6N: N=7 mice, n=13 slices; 129, N=7 mice, n=13 slices) in B6N
strain. (n) No statistical differences were detected for
CA3-CA1 cross correlation of gamma oscillations (B6J: N=8 mice, n=18
slices; B6N: N=7 mice, n=13 slices; 129, N=7 mice, n=13 slices).
Statistical comparison for (d), (e), (h), (i), (l), (m):one-way-ANOVA followed by posthoc comparison using Fisher LSD Method.
Statistical comparison for (f), (j), (n): Kruskal-Wallis One
Way Analysis of Variance on Ranks followed by posthoc comparison using
Dunn´s Method. Post-hoc statistical differences are indicated via
*p<0.05 and **p<0.01. Data are presented ± standard
error of mean (SEM). Empty circles represent individual data points.