6.1.2 Erythrosine B dye exclusion assay
The main application for erythrosine B is as a food colouring additive.
It is also known as Red No. 3 or erythrosine. An essential dye used to
count living cells is erythrosine B (Marmion and Updated by Staff,
2012). The foundation of this colour exclusion test is comparable to
that of the trypan blue test. Even though erythrosin B is a biosafe
vital dye for cell counts, determining how many live and dead cells are
present in a sample is not a common practise. Low cost, adaptability,
and bio-safety are just a few of the benefits of the test(Kim et al.,
2016).The time and labour-intensive nature of this method are its
drawbacks. Furthermore, impurity of the reusable cell counting chamber,
differences in haemocytometer filling rates, and inter-user variances
are also potential drawbacks(Kim et al., 2016).