6.1.2 Erythrosine B dye exclusion assay
The main application for erythrosine B is as a food colouring additive. It is also known as Red No. 3 or erythrosine. An essential dye used to count living cells is erythrosine B (Marmion and Updated by Staff, 2012). The foundation of this colour exclusion test is comparable to that of the trypan blue test. Even though erythrosin B is a biosafe vital dye for cell counts, determining how many live and dead cells are present in a sample is not a common practise. Low cost, adaptability, and bio-safety are just a few of the benefits of the test(Kim et al., 2016).The time and labour-intensive nature of this method are its drawbacks. Furthermore, impurity of the reusable cell counting chamber, differences in haemocytometer filling rates, and inter-user variances are also potential drawbacks(Kim et al., 2016).