6.3 Fluorometric assays
The fluorometric studies for determining cell viability and cytotoxicity have many benefits over traditional dye exclusion and colorimetric investigations when carried out with a fluorescence microscope, fluorimeter, fluorescence microplate reader, or flow cytometer. Tests using fluorescence can be used with adherent or suspension cultures. Tests using colorimetry are less accurate than these assays. Commercial fluorometric test kits are available from a number of manufacturers, and the majority of the experimental methods for these tests are included in kit packages(Vollmer, 2003).