6.4 Luminometric assays
The mammalian cell cytotoxicity and proliferation can be quickly and
easily determined using luminometric methods. Utilizing luminometric
microplate readers, these tests can be run on microplates with 96 or 384
wells. The consistent and persistent glow-type signal that is generated
after reagent addition is a distinguishing feature of luminometric
tests. Using this characteristic, viability and cytotoxicity values can
be generated from the same well (Niles et al., 2009). The commercial
luminometric assay kits are commercially avaialable, and the
experimental methods for these assays are usually included with the kit
packages.