2.3.2 Two stage models for skin tumorigenesis
Tumor induction is performed in this model in two stages: initiation and promotion. A single topical injection of dimethyl benzanthrazene (DMBA) (50 nMol) is used to initiate the treatment, which is then followed by two weekly UV light treatments (250 mJ/cm2) for a total of 25 weeks. These models have been used in studies to assess the process of carcinogenesis. Certain test medicines’ modes of action can also be investigated. For example, whether the drug can stop or stop the process of start or promotion(Gali-muhtasib et al., 2009).