6.3 Fluorometric assays
The fluorometric studies for determining cell viability and cytotoxicity
have many benefits over traditional dye exclusion and colorimetric
investigations when carried out with a fluorescence microscope,
fluorimeter, fluorescence microplate reader, or flow cytometer. Tests
using fluorescence can be used with adherent or suspension cultures.
Tests using colorimetry are less accurate than these assays. Commercial
fluorometric test kits are available from a number of manufacturers, and
the majority of the experimental methods for these tests are included in
kit packages(Vollmer, 2003).