6.4 Luminometric assays
The mammalian cell cytotoxicity and proliferation can be quickly and easily determined using luminometric methods. Utilizing luminometric microplate readers, these tests can be run on microplates with 96 or 384 wells. The consistent and persistent glow-type signal that is generated after reagent addition is a distinguishing feature of luminometric tests. Using this characteristic, viability and cytotoxicity values can be generated from the same well (Niles et al., 2009). The commercial luminometric assay kits are commercially avaialable, and the experimental methods for these assays are usually included with the kit packages.