2.1. Culture conditions and Materials of FACHB-314
Spirulina FACHB-314 was purchased from Wuhan Institute of Aquatic
Plants, Chinese Academy of Sciences. Zarrouk medium (Lebron et al.,
2018; Liang et al., 2018) is used as a medium for FACHB-314experiments, composition (g/L: NaHCO3 16.8,
NaNO3 2.5, K2SO4 1.00,
NaCl 1.00, MgSO4·7H2O 0.20,
FeSO4·7H2O 0.01,
K2HPO4 0.50,
CaCl2·2H2O 0.04, EDTA 0.08, 1mL of trace
elements. Among them, trace element composition (g/L):
H3BO3 2.86,
MnCl2·4H2O 1.81,
ZnSO4·4H2O 0.22, NaMoO40.018, CuSO4·5H2O 0.08.
Inoculate FACHB-314 into the reactor and put it in the smart
light incubator (Khanra et al., 2018). The structure of the reactor is
shown in Scheme 1. The photobioreactor is a 1 L conical flask with a
bottom radius of 9 cm and a height of 20 cm. Place the photobioreactor
in a smart light incubator, keep the temperature at 25°C, and
continuously supply air at a ventilation rate of 0.7 vvm (Karthikeyan et
al., 2018a). In an Erlenmeyer flask containing TiO2 and
rGO-TiO2, 50 mg of nanomaterials were added for
co-cultivation.