5. Discussion
This topic focuses on the promotion of the addition of
rGO-TiO2 nanoparticles on the growth of FACHB-314platensis and the accumulation of intracellular phycocyanin. Through a
series of experimental optimization and exploration, the following
conclusions are drawn:
In this experiment, we designed and prepared reduced graphene oxide
titanium dioxide nanoparticles (rGO-TiO2) with good
biocompatibility, which were used in the cultivation of FACHB-314platensis to enhance the absorption and utilization of light, compared
with conventional TiO2 Nanoparticles,
rGO-TiO2 nanoparticles have high biocompatibility, have
no significant inhibitory effect on the growth of FACHB-314 , and
have no obvious damage to the morphology and structure. To study the
effects of rGO-TiO2 nanoparticles on the growth ofA. platensis and the accumulation of phycocyanin under different
irradiation light colors, light-dark cycles, and light intensity
conditions.
Under the same conditions, compared with the TiO2nanoparticles and the control group, the addition of
rGO-TiO2 showed a greater promotion advantage. By the
seventh day of culture, the maximum phycocyanin content was 80.3 mg/g,
and the intracellular phycocyanin production at 97.16 mg/L, the
production of phycocyanin increased by 18.7%. Compared with theFACHB-314 cultured with TiO2 nanoparticles, the
phycocyanin content increased by 24.6 mg/g compared with the
experimental group with TiO2 nanoparticles, and the
content increased by 44.2%. On the ninth day of culture, the cell dry
weight reached the maximum value of 1.66 g/L, and TiO2was 0.65 g/L.
We found that in the choice of different wavelengths, the cell growth ofA. platensis FACHB-314 and the accumulation of phycocyanin
are more biased towards the white light. Under different light-dark
cycles, full light has a more obvious promotion effect on the cell
growth of A. platensis and the accumulation of phycocyanin. Under
different light intensities, we found that high light intensity is more
beneficial to the growth of FACHB-314 , while the accumulation of
phycocyanin prefers low light intensity. Compared with the control group
cultured without nanoparticles, A. platensis FACHB-314doped with rGO-TiO2 nanoparticles can promote the
accumulation of phycocyanin.