2.3. Determination of FACHB-314 Cell Growth
Accurately weigh the 50 ml centrifuge tube and mark it as
M0. Transfer 20 ml of algae solution and centrifuge for
10 minutes (Caporgno & Mathys, 2018). Leave the sediment and wash it
several times, and finally dry it to a constant weight, weigh it as
M1, and then calculate the dry battery weight DCW (g/L)
according to Eqs (1) Measure the absorbance value at 680nm using an
ultraviolet-visible spectrophotometer.
The absorbance value multiplied by the dilution factor is the
OD680 of FACHB-314 . The relationship between
battery dry weight DCW (g / L) and OD680 is Eqs (2):
\(DCW(g/L):\ M=1000\times(M1-M0)\div 20\) (1)
\(DCW(g/L)=0.5192\text{OD}_{680}\pm 0.01002(R^{2}=0.99779)\) (2)