2.2. Assessment of antioxidant capacity
The Ag NPs/CS-Starch bio-nanocomposite in 11 variable concentrations (1-1000 µg/ml, 1 ml each) was introduced to DPPH methanolic solution (1ml, 300 µM) and the tptal volume of the mixture was made up to 4000 µl with MeOH. Subsequently, the falcons were vortexed and remained in dark for 30 min. Finally, the color change due to radical quenching was monitored spectrophotometrically at a wavelength of 517 nm. The scavenging capacity of DPPH radical expressed in terms of % inhbition was determined by the following equation:
I (%) = (A0-AS)/A0×100
Where, A0 is the absorption of the control (containing all reactive components without sample and AS is the absorption of the sample) [13,14].