4. Conclusions
In summary, we have represented a bio-inspired method for the synthesis of stable Ag NPs being encapsulated and stabilized over pre-designed chitosan-starch bio-composite hydrogel. The procedure involved sustainable protocols and free from any hazardous and toxic chemicals. The material was analyzed towards its physicochemical characterizations following a wide range of technical methods. The Ag NPs formed were almost monodisperse in dimension in the range of 5-15 nm. They are uniformly distributed without any aggregations. The material was directed in the bio-analysis for antioxidant studies and its efficiency in the inhibition of different kinds of leukemia cell lines. In the antioxidant study the material in different concentration was investigated to quench the DPPH free radicals and was found to produce outstanding results. Again, the cytotoxicity analysis by MTT assay over the 6 leukemia cell lines were also carried out at different sample concentrations. The % cell viability of the same was found to decrease dose-dependently. However, the normal cell lines was found to be unaffected in the same study. This justifies the Ag NPs/CS-Starch nanocomposite could be considered as a competent nano-formulated drug candidate against different leukemia cancers. Further research on this study would be greatful in discovering a new dimension in the cancer research.