2.2. Assessment of antioxidant capacity
The Ag NPs/CS-Starch bio-nanocomposite in 11 variable concentrations
(1-1000 µg/ml, 1 ml each) was introduced to DPPH methanolic solution
(1ml, 300 µM) and the tptal volume of the mixture was made up to 4000 µl
with MeOH. Subsequently, the falcons were vortexed and remained in dark
for 30 min. Finally, the color change due to radical quenching was
monitored spectrophotometrically at a wavelength of 517 nm. The
scavenging capacity of DPPH radical expressed in terms of % inhbition
was determined by the following equation:
I (%) = (A0-AS)/A0×100
Where, A0 is the absorption of the control (containing
all reactive components without sample and AS is the
absorption of the sample) [13,14].