Fig. 7. The antioxidant properties of
nanocomposite and BHT against
DPPH.
Furthermore, in the cytotoxic assays following MTT study the
experimental nanosample was treated to different leukemia cell lines at
several concentrations. The analysis was done for 48 h against acute
myeloid leukemia (32D-FLT3-ITD and Human HL-60/vcr), acute lymphoblastic
leukemia (MOLT-3 and TALL-104), and acute T cell leukemia (Jurkat, Clone
E6-1 and J.RT3-T3.5) cell lines (Figures 8-10). The material is safe to
the normal cell line, HUVEC, and remains uaffected, being showed in Fig.
11. Interestingly, the % cell viability of malignant leukemic cell
lines against the nano-formulated drug reduced with increasing
concentrations or doses. The yellow colored MTT solution gets reduced to
purple colored formazan crystal which is measured spectrophotometrically
at 570 nm. The corresponding IC50 values in the MTT assay against the
cell lines were observed as 203, 301, 250, 227 and 102, 193
respectively.