Fig. 7. The antioxidant properties of nanocomposite and BHT against DPPH.
Furthermore, in the cytotoxic assays following MTT study the experimental nanosample was treated to different leukemia cell lines at several concentrations. The analysis was done for 48 h against acute myeloid leukemia (32D-FLT3-ITD and Human HL-60/vcr), acute lymphoblastic leukemia (MOLT-3 and TALL-104), and acute T cell leukemia (Jurkat, Clone E6-1 and J.RT3-T3.5) cell lines (Figures 8-10). The material is safe to the normal cell line, HUVEC, and remains uaffected, being showed in Fig. 11. Interestingly, the % cell viability of malignant leukemic cell lines against the nano-formulated drug reduced with increasing concentrations or doses. The yellow colored MTT solution gets reduced to purple colored formazan crystal which is measured spectrophotometrically at 570 nm. The corresponding IC50 values in the MTT assay against the cell lines were observed as 203, 301, 250, 227 and 102, 193 respectively.