Man Li*
Department of Endoscopy, Harbin Medical University Cancer Hospital,
Harbin, Heilongjiang, 150081, China, Pre-test triage, The Fourth
People’s Hospital of Jinan, Jinan city, China.
* Corresponding author: dr.man.li.12345@gmail.com
Abstract. We herein demonstrate the biogenic
nanoarchitechtonics of silver nanoparticles template over
chitosan/starch mixed hydrogel having excellent reducing ability. The
two biopolymers also had the capacity to stabilize as-synthesized Ag
NPs. Physicochemical and structural features of the nanocomposite
biomaterial was assessed by several techniques like FT-IR, SEM, TEM, EDX
and XRD. TEM study revealed the mean diameter of the spherical shaped Ag
NPs/CS-Starch material was in the range of 5-15 nm. Thereafter, the Ag
NPs/CS-Starch bio-composite material was exploited in the study
of cytotoxicity and anti-leukemia effects against diverse leukemia cell
lines like acute myeloid leukemia (32D-FLT3-ITD and Human HL-60/vcr),
acute lymphoblastic leukemia (MOLT-3 and TALL-104), and acute T cell
leukemia (Jurkat, Clone E6-1 and J.RT3-T3.5) in situ .
Interestingly, the nano-drug could resist significantly against those
cell lines in a time and concentration-dependent manner, assessed by MTT
method. The corresponding IC50 values of the
bio-composite were 203, 301, 250, 227, 102, and 193 µg/mL respectively
against the cell lines. Furthermore, antioxidant potential of the
material was investigated by DPPH radical scavenging method. A
significantly high IC50 value suggested the high
antioxidant capacity of Ag NPs/CS-Starch nanomaterial.
Keywords: Chitosan-Starch, Silver nanoparticles, cytotoxicity,
Leukemia, Antioxidant.