4. Conclusions
In summary, we have represented a bio-inspired method for the synthesis
of stable Ag NPs being encapsulated and stabilized over pre-designed
chitosan-starch bio-composite hydrogel. The procedure involved
sustainable protocols and free from any hazardous and toxic chemicals.
The material was analyzed towards its physicochemical characterizations
following a wide range of technical methods. The Ag NPs formed were
almost monodisperse in dimension in the range of 5-15 nm. They are
uniformly distributed without any aggregations. The material was
directed in the bio-analysis for antioxidant studies and its efficiency
in the inhibition of different kinds of leukemia cell lines. In the
antioxidant study the material in different concentration was
investigated to quench the DPPH free radicals and was found to produce
outstanding results. Again, the cytotoxicity analysis by MTT assay over
the 6 leukemia cell lines were also carried out at different sample
concentrations. The % cell viability of the same was found to decrease
dose-dependently. However, the normal cell lines was found to be
unaffected in the same study. This justifies the Ag NPs/CS-Starch
nanocomposite could be considered as a competent nano-formulated drug
candidate against different leukemia cancers. Further research on this
study would be greatful in discovering a new dimension in the cancer
research.