2.1 Materials
Iso -oleic acid ( 78 wt.% iC18:1, 13 wt.% C18:0, 9.0 wt.% lactones) were synthesized according to the procedures reported in Ngo et al. (2012) publication. Lauric acid (97% C12:0) was purchased from Ward’s Science (Rochester, NY). Oleic acid (91 wt.% C18:1), celite®, thionyl chloride, dichloromethane, dimethylformamide (DMF), triethylamine, L-Arginine, and L-arginine ethyl hydrochloride were purchased from Sigma Aldrich (Saint Louis, MO). LAE was obtained from Ambeed (Arlington Heights, IL). Palladium on carbon (5 wt% Pd/ C) was purchased from Pressure Chemical Co. (Pittsburgh, PA). Deuterated methanol and deuterochloroform were from Cambridge Isotope Laboratories, Inc. (Tewksbury, MA). L. innocua ATCC 33090 and E. coli ATCC 700728 were purchased from American Type Culture Collection (Manassas, VA). Tryptic soy broth (TSB) and tryptic soy agar (TSA) were from BD (Franklin Lakes, NJ).