Figure 3. Phenotyping and genotyping data of the recombined individuals in the (A-C) BC2F2 population, (D) F2 segregation population derived from the mutant and the wild line and (E) germplasm materials in Brassica oleracea .
Note: (A) indicates phenotyping and genotyping of 96 individual plants with recombined chromosome segment in the target. (B) and (C) depict further genotyping of the recombined individuals based on (A) and B with new KASP markers, respectively. (D) and (E) show the application of the relative KASP markers. ‘G’ with green background and ‘P’ with purple background indicate buds-keeping-green trait (KGLT) and buds-turning-purple trait at low temperature (TPLT), respectively. ‘0’ (light green background) and ‘1’ (dark violet background) represent the homozygous genotypes same as the paternal and maternal line, respectively, and ‘2’ with light violet background is for heterozygous genotype.