Figure 3. Phenotyping and genotyping data of the recombined individuals
in the (A-C) BC2F2 population, (D)
F2 segregation
population derived from the mutant and the wild line and (E) germplasm
materials in Brassica oleracea .
Note: (A) indicates phenotyping and genotyping of 96 individual plants
with recombined chromosome segment in the target. (B) and (C) depict
further genotyping of the recombined individuals based on (A) and B with
new KASP markers, respectively. (D) and (E) show the application of the
relative KASP markers. ‘G’ with green background and ‘P’ with purple
background indicate buds-keeping-green trait (KGLT) and
buds-turning-purple trait at low temperature (TPLT), respectively. ‘0’
(light green background) and ‘1’ (dark violet background) represent the
homozygous genotypes same as the paternal and maternal line,
respectively, and ‘2’ with light violet background is for heterozygous
genotype.