Expression profile of anthocyanins relative gene in the wild line at different temperature
43 genes relative with anthocyanin biosynthesis were found to express in the buds of broccoli PS, which was accordant with the above result. The expression of most of the anthocyanins relative genes was regulated by low temperature. Among 43 genes, the expression of 29 genes were regulated by low temperature, out of which 20 genes were up-regulated, and 9 down-regulated at low temperature (Figure 5). One of sixPALs , four of five C4Hs , one F3H , one F3’H , and one of two FLS showed higher expression at low temperature than at room temperature, whereas both ANS and one of threeCHI were down-regulated. And out of ten 4CLs , four4CLs were up-regulated at low temperature, and four showed lower expression. Out of four CHSs , three CHSs expressed higher at low temperature and one lower. There were 6 regulating factors that were differentially expressed, five of which were up- regulated by low temperature and one was down-regulated (Table S6).