The CDS sequences and a phylogenetic analysis of F3’H
The buds of HX-11 and HX-16 were collected 10 days after experiencing cold air (0-50 C) and frozen in liquid nitrogen, and preserved in a -80℃ refrigerator. The RNA was extracted from the samples using the Trizol Reagent (Invitrogen Life Technologies, Carlsbad, USA). The cDNA was synthesized from mRNA using TIANScript Kit (KR104) (Tiangen Biotech Co., LTD, Beijing) according to the instruction. The primers were designed based on the sequence of F3’H CDS in the HDEM reference genome to amplify the CDS sequences of HX-11 and HX-16 (Table S7). The amino acid sequence of F3’H in Arabidopsis thaliana was aligned in NCBI. Then the highly identical amino acid sequences were downloaded and used for phylogenetic analysis by MEGA X.