The CDS sequences and a phylogenetic analysis of F3’H
A 43-bp deletion was detected in the CDS sequence of HX-16, whose buds
would keep green in cold temperature, compared with the maternal line,
HX-11, whose buds turn purple in cold temperature. The CDS sequence of
HX-11 had 2 SNPs compared with the reference genome HDEM. A phylogenetic
analysis was conducted using the amino acid sequences of the F3’Hgene deduced from some plants of Brassica, Raphanus, Arabidopsis,
Sinapis, Isatis, Eutrema, Camelina, Capsella and Matthiola incana . The
phylogenetic analysis showed that the F3’H gene with a deletion
was grouped into the same clade as the F3’H from the reference
genome HDEM, and the F3’H s from Brassica, Raphanus,
Arabidopsis, Camelina and Matthiola were classified into a
different group, indicating that the amino acid sequences of F3’Howned genus-specific conservation (Figure 6).