Cell counts
Total nucleated counts were obtained using an automated Navios EX Flow
Cytometer and Kaluza Analysis Software (Beckman Coulter Inc., Miami, FL,
USA). Flow cytometry analysis was performed using appropriate monoclonal
antibodies that recognize CD45+ (KrO), CD3+(FITC), CD34+(PE), CD8+
(PC7), CD4+ (PC5.5), CD19 (ECD) and CD16+ (PE) cells ( Beckman Coulter
Inc., Marseille, France). The number of CD34+ cells infused was
calculated as the % of antigens and a FAC Sort instrument from
CD34+/CD45+ cells × the total nucleated count.