Cell counts
Total nucleated counts were obtained using an automated Navios EX Flow Cytometer and Kaluza Analysis Software (Beckman Coulter Inc., Miami, FL, USA). Flow cytometry analysis was performed using appropriate monoclonal antibodies that recognize CD45+ (KrO), CD3+(FITC), CD34+(PE), CD8+ (PC7), CD4+ (PC5.5), CD19 (ECD) and CD16+ (PE) cells ( Beckman Coulter Inc., Marseille, France). The number of CD34+ cells infused was calculated as the % of antigens and a FAC Sort instrument from CD34+/CD45+ cells × the total nucleated count.