BM priming and collection
All donors received G-CSF 10 µg/kg daily as a single subcutaneous
injection at least 24 hours before BM harvesting (Day -1). In addition,
hemogram was checked using a peripheral blood sample before G-CSF was
administered to all donors. At 24 hours, immediately before collecting
the BM product, another hemogram was measured from a peripheral blood
sample. The patients in the control group were not administered with any
G-CSF. On Day 0, BM was collected from both posterior iliac bones under
general anesthesia in line with the standard training procedures, aiming
a target volume of 10-20 mL per kg body weight of the recipient and not
exceeding 20 mL per kg body weight of the donor. BM was collected by
volume and mid-collection cell counts were not performed. HSC collection
was performed in a single session for all patients. All donors were
fully assessed for any events related to G-CSF use or BM harvesting
prior to donation, on G-CSF administration days and after BM harvesting
until discharge from hospital.