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\begin{document}

\title{The complete mitochondrial genomes of two moths in the tribe Trichaeini
(Lepidoptera: Crambidae) and the phylogenetic implications for
Pyraloidea}



\author[1]{Xicui Du}%
\author[1]{Ci Tang}%
\affil[1]{Southwest University}%


\vspace{-1em}



  
  \date{June 05, 2024}


\begingroup
\let\center\flushleft
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\endgroup





\selectlanguage{english}
\begin{abstract}
The complete mitochondrial genomes of two Prophantis Warren, 1896
species in the tribe Trichaeini (Lepidoptera: Crambidae) were sequenced
using high-throughput sequencing technology. They were assembled and
annotated: the complete mitogenomes of P. octoguttalis and P. adusta
were 15,197 bp and 15,714 bp, respectively, and contain 13
protein-coding genes, 22 transfer RNA genes, two ribosomal RNA genes,
and an A + T-rich region. Their arrangement was consistent with the
first sequenced mitogenome of Lepidoptera, from Bombyx mori
(Bombycidae). The nucleotide composition was obviously AT-biased, and
all protein-coding genes, except for the cox1 gene (CGA), used ATN as
the start codon. Except for trnS1, which lacked the DHU arm, all tRNA
genes could fold into the clover-leaf structure. Phylogenetic trees of
Pyraloidea were reconstructed based on mitogenomic data using Maximum
likelihood (ML) and Bayesian inference (BI) analysis methods. The
results showed that Trichaeini formed a monophyletic group with high
branch support in Spilomelinae, sister to Nomophilini. In addition, the
phylogenetic relationships among subfamilies of Pyraloidea were
generally stable: (Galleriinae + ((Epipaschiinae + Pyralinae) +
Phycitinae)) + ((Pyraustinae + Spilomelinae) + ((Odontiinae +
Glaphyrinae) + CAMMSS clade)), although the affinities of some
subfamilies in the ``CAMMSS clade'' were still unresolved.%
\end{abstract}%



\sloppy


\textbf{The complete mitochondrial genomes of two moths in the
tribeTrichaeini (Lepidoptera: Crambidae) and the phylogenetic
implications for Pyraloidea}

Ci Tang | Xicui Du

Laboratory of Lepidoptera Systematics, College of Plant Protection,
Southwest University, Chongqing 400715, China

\textbf{Correspondence}

Xicui Du, Laboratory of Lepidoptera Systematics, College of Plant
Protection, Southwest University, Chongqing 400715, China. Email:
duxicui@hotmail.com, duxicui@swu.edu.cn

\textbf{Funding information}

This work was sponsored by the National Natural Science Foundation of
China (No. 31772500) and by Natural Science Foundation of Chongqing,
China (No. 2022NSCQ-MSX3668)

\textbf{Abstract}

The complete mitochondrial genomes of two \emph{Prophantis} Warren, 1896
species in the tribe Trichaeini (Lepidoptera: Crambidae) were sequenced
using high-throughput sequencing technology. They were assembled and
annotated: the complete mitogenomes of \emph{P. octoguttalis}
and\emph{P. adusta} were 15,197 bp and 15,714 bp, respectively, and
contain 13 protein-coding genes, 22 transfer RNA genes, two ribosomal
RNA genes, and an A + T-rich region. Their arrangement was consistent
with the first sequenced mitogenome of Lepidoptera, from \emph{Bombyx
mori}(Bombycidae). The nucleotide composition was obviously AT-biased,
and all protein-coding genes, except for the \emph{cox1} gene (CGA),
used ATN as the start codon. Except for \emph{trnS1} , which lacked the
DHU arm, all tRNA genes could fold into the clover-leaf structure.
Phylogenetic trees of Pyraloidea were reconstructed based on mitogenomic
data using Maximum likelihood (ML) and Bayesian inference (BI) analysis
methods. The results showed that Trichaeini formed a monophyletic group
with high branch support in Spilomelinae, sister to Nomophilini. In
addition, the phylogenetic relationships among subfamilies of Pyraloidea
were generally stable: (Galleriinae + ((Epipaschiinae + Pyralinae) +
Phycitinae)) + ((Pyraustinae + Spilomelinae) + ((Odontiinae +
Glaphyrinae) + CAMMSS clade)), although the affinities of some
subfamilies in the ''CAMMSS clade'' were still unresolved.

\textbf{Keywords}

mitogenome; phylogeny; \emph{Prophantis} ; Spilomelinae

\textbf{1 Introduction}

The Pyraloidea, with more than 16,000 described species worldwide, is
one of the largest groups in Lepidoptera, and is composed of two
families, Pyralidae and Crambidae (Munroe \& Solis 1999, Nuss et al.,
2003--2022). There are many important agricultural pests in this group,
such as \emph{Cnaphalocrocis medinalis} (Guen\selectlanguage{ngerman}ée, 1854), \emph{Ostrinia
nubilalis} (Hübner, 1796),\emph{Maruca vitrata} (Fabricius, 1787),
\emph{Spoladea recurvalis}(Fabricius, 1775), etc. In the present
systematics of Pyraloidea, Crambidae, comprising about 60\% of
Pyraloidea, includes 15 subfamilies. Phylogenetic relationships within
Crambidae were revealed based on ten genes totalling 11,247 bp by Léger
et al. (2021): (Pyraustinae + Spilomelinae) + ((Odontiinae + (Linostinae
+ Glaphyrinae)) + ((Lathrotelinae + Musotiminae) + ((Midilinae +
(Schoenobiinae + Acentropinae)) + (Hoploscopinae + ((Erupinae +
(Heliothelinae + Scopariinae)) + Crambinae))))), where the sister group
relationship of Pyraustinae and Spilomelinae has also been demonstrated
in previous studies using molecular and morphological data (Regier et
al., 2012; Ma et al., 2016; Yang et al., 2018 a and b; Mally et al.,
2019; Zhang et al., 2020; Liu et al., 2021). Pyralidae comprises five
subfamilies: Chrysauginae, Epipaschiinae, Galleriinae, Phycitinae, and
Pyralinae (Regier et al., 2012; Léger et al., 2021).

Spilomelinae is the most species-rich subfamily in Crambidae, with 4,132
described species in 340 genera (Léger et al., 2021). A total of 13
tribes have been defined for Spilomelinae (Mally et al., 2019):
Hydririni + ((Udeini + Lineodini) + (Wurthiini + (Agroterini +
(Margaroniini + (Spilomelini + (Herpetogrammatini + ((Hymeniini +
Asciodini) + (Trichaeini + (Steniini + Nomophilini))))))))). Among them,
Trichaeini is a tribe with less species richness, with only four genera
and 22 species. This clade was established by Mally et al. (2019) in a
phylogenetic analysis of Spilomelinae based on six molecular markers and
114 adult morphological characters. This tribe includes the
genus\emph{Prophantis} Warren, 1896, which consists of eight species
that have all been poorly studied besides their original
descriptions.\emph{Prophantis octoguttalis}Felder \& Rogenhofer, 1875
and \emph{P. adusta} Inoue, 1986 have been recorded from China.
\emph{Prophantis octoguttalis} , the type species of the genus, is
widespread, and is mainly distributed in southern China, Australia,
India, and the Afrotropical region. Its larvae feed on\emph{Coffea
arabica} Linneaus, 1757, and a single larva can harm several berries in
succession, which can seriously impact coffee production. The adults of
\emph{P. adusta} are very similar in appearance to those of \emph{P.
octoguttalis} , which can make identifying these moths very challenging.

The mitochondrial genome (mtDNA) is mostly a closed-loop DNA double
helix molecule that varies significantly in length among taxa. The mtDNA
of lepidopteran insects is generally 15--16 kb in size and consists of
37 genes, including 13 protein-coding genes (PCGs), 22 transfer RNA
genes (tRNAs), two ribosomal RNA genes (rRNAs), and a noncoding region
of variable length (Boore, 1999). Because of its conserved genetic
components, compact arrangement, fast evolutionary rate, and maternal
inheritance, it contains relevant genetic and developmental information
that can be used in phylogenetic studies for different research purposes
(Wesley et al., 1979; Cameron, 2014). The mtDNA has been widely used in
research on the origin and evolution of species, molecular phylogeny,
lineage geography, and relationship between mitochondrial genes and
diseases (Taanman, 1999; Johns, 1995; Suzuki et al., 2013).

To date, only 23 mitogenomes of Spilomelinae have been published in
GenBank, and no mitogenomes of Trichaeini have been reported. In this
study, we sequenced the mitogenomes of \emph{P. octoguttalis}
and\emph{P. adusta} of the Trichaeini for the first time, and performed
preliminary bioinformatics analysis. Combined with the 72 sequences of
Pyraloidea obtained from NCBI, we reconstructed the phylogenetic tree of
Pyraloidea by using Maximum likelihood and Bayesian inference methods.
The aim was to provide genomic data and genomic characteristics, and to
provide a scientific basis for phylogenetic research of Trichaeini.

\textbf{2 Materials and methods}

2.1 Specimen collection and DNA sequencing

\emph{Prophantis octoguttalis} was collected from Wuzhi Mountain in
Hainan Province, China, in March 2021; \emph{P. adusta} was collected
from Fanjing Mountain in Guizhou Province, China, in September 2020.
Fresh specimens obtained by light trapping were soaked in anhydrous
alcohol and stored at -80 °C in the Insect Collection of Southwest
University, Chongqing, China. DNA was extracted from the thoracic muscle
of each specimen. The mitogenome was entrusted to BGI Genomics for
next-generation sequencing.

2.2 Sequence assembly, annotation and analysis

The high-quality data (clean data) of the samples, which were trimmed by
BGI Genomics, were saved as fastq. format and imported into Geneious
Prime v2022.1.1. The mitogenome with the closest affinity to the sample
as a reference sequence was downloaded from GenBank, and sequence
extension was performed using the ``Map to reference'' function until
repetitive base alignments appeared, indicating that the mitochondrial
genome was assembled into a loop.

MAFFT (Multiple Alignment using Fast Fourier Transform) alignment was
used to align the reference sequence with the sample sequence, and
protein-coding genes (PCGs) were determined based on the similarity
between genes. With the help of EditSeq v7.1.0, PCGs were translated
into amino acids to further verify the correctness of the start codon,
stop codon, and amino acid sequence, to ensure the accuracy of PCGs.
tRNA genes were predicted using the MITOS Web Server (Donath et al.,
2019), and their secondary structure was mapped using Adobe Illustrator
v26.0. rRNA genes are relatively conserved, and can be determined by the
position between the two genes (Boore, 2006). The A+T-rich region was
generally located behind the \emph{rrnL} gene. Mitogenome maps were
generated using Proksee (https://proksee.ca/). Sequence length, base
composition, gene spacing, and overlap were viewed directly using
Geneious Prime v2022.1.1. The base skew was calculated using the
formula: AT skew = (A \selectlanguage{english}- T) / (A + T) and GC skew = (G \selectlanguage{english}- C) / (G + C)
(Perna and Kocher, 1995). Relative synonymous codon usage (RSCU) was
analyzed using MEGA v10.2.5.

2.3 Phylogenetic analysis

A total of 74 mitogenome sequences were used as ingroups, including 25
Pyralidae and 49 Crambidae species. One Bombycidae and one Noctuidae
species were selected as outgroups (Table 1). We used four datasets: 1)
PCG123: all three codon positions of 13 protein-coding genes; 2)
PCG123R: all three codon positions of 13 protein-coding genes and two
rRNA genes; 3) PCG123RT: 37 genes of the mitogenome; 4) PCG12: first and
second codon positions of 13 protein-coding genes. Maximum likelihood
(ML) and Bayesian inference (BI) were used to construct phylogenetic
trees.

PartitionFinder 2 (Lanfear et al., 2016) was used to find the best
partitioning scheme and base substitution models for ML and BI, with the
following parameters: the model parameters were set to ``mrbayes,'' the
branch length was selected to ``linked,'' and the ``greedy'' strategy
was used to search; Bayesian Information Criterion (BIC) was used to
partition the data; and the three codon sites of the protein-coding
genes were represented by pos1, pos2, and pos3, respectively. Maximum
likelihood was analyzed using IQ-TREE v1.6.8 (Minh et al., 2013; Nguyen
et al., 2015), with Ultrafast bootstrap of 1000 replications; bootstrap
values (BS) \emph{\textgreater{}} 70\% were considered to represent high
confidence. Bayesian inference was analyzed using MrBayes v3.2.6, with
the following parameters: two independent runs, each with four
independent Markov Chain Monte Carlo runs, including three heated chains
and one cold chain, were set to run for 1 x
10\textsuperscript{7}generations, with simultaneous sampling every 1,000
generations. The initial 25\% of the sampled trees were discarded as
burn-ins. Chain convergence was assumed when the mean standard deviation
of the split frequencies fell below 0.01. Bayesian posterior
probability, in which the support of each node of the BI tree was
greater than or equal to 0.95, was considered high confidence. The
phylogenetic tree was constructed using Figtree v.1.4.4.

\textbf{3 Results and discussion}

3.1 Basic structure

The full length of the mitochondrial genomes of \emph{Prophantis
octoguttalis} and \emph{P. adusta} were 15,197 bp and 15,714 bp,
respectively, including 37 genes and non-coding regions (Figure 1). Four
protein-coding genes (\emph{nad1} , \emph{nad4} , \emph{nad5} ,
and\emph{nad4l} ), two rRNA genes (\emph{rrnL} and \emph{rrnS} ), and
eight tRNA genes (\emph{trnQ} , \emph{trnC} , \emph{trnY} , \emph{trnF}
,\emph{trnH} , \emph{trnP} , \emph{trnL1} , and \emph{trnV} ) were
encoded by the minority strands. The remaining 23 genes were encoded by
the majority of the strands (Table 2). The mitogenomes of both species
were arranged in the same order as that of \emph{Bombyx mori} (Linnaeus,
1758), which is the model organism in Lepidoptera. There were eight gene
overlaps and 15 gene gaps in the mitogenome of \emph{P. octoguttalis} ,
while five genes overlapped and 18 gene gaps were found in the
mitogenome of \emph{P. adusta} .

\textbf{Figure 1.} Visualization of the mitochondrial genomes
of\emph{Prophantis octoguttalis} and \emph{P. adusta}

The mitogenome sequences of both species showed obvious AT biases. The
nucleotide content of the \emph{P. octoguttalis} mitogenome was A:
41.0\%, T: 40.5\%, C: 11.0\%, and G: 7.5\%, and for the \emph{P.
adusta}mitogenome was A: 40.8\%, T: 40.7\%, C: 11.0\%, and G: 7.4\%. The
AT contents were 81.5\% and 81.6\%, respectively, which were much higher
than the GC content. The AT skew was 0.006 and 0.001, and the GC skew
was -0.189 and -0.196, respectively, showing a slight A skew and a
significant C skew (Table 3).

3.2 Protein-coding genes and codon usage

Thirteen protein-coding genes were identified in the mitogenomes of
\emph{P. octoguttalis} and\emph{P. adusta} . Among them, \emph{atp8} ,
\emph{atp6} , \emph{cox1} ,\emph{cox2} , \emph{cox3} , \emph{nad2} ,
\emph{nad3} , \emph{nad6} , and\emph{cytb} were encoded by the majority
strand, and the remaining four genes (\emph{nad1} , \emph{nad4} ,
\emph{nad5} , and \emph{nad4l} ) were encoded by the minority strand.
The start codons of all genes were typical ATN (ATT, ATA, ATG), except
for \emph{cox1} , whose start codon was CGA. The stop codons of
\emph{cox1} and \emph{cox2} in \emph{P. octoguttalis} were terminated by
an incomplete stop codon T, and the remaining genes were terminated by
TAA, which was the most frequent stop codon. Among the protein-coding
genes, the AT content was 80.3\% and 79.6\%, respectively. The AT bias
of these two species was more significant in the third codon, and the AT
content of the third codon (83.2\%, 85.8\%) was higher than that of the
first (73.1\%, 82.7\%) and second codons (74.9\%, 79.8\%). The AT skew
of these two species was 0.01 and 0.003, and their GC skew was -0.173
and -0.181, respectively, showing a slight A skew and an obvious C skew.

\textbf{Figure 2.} Relative synonymous codon usage (RSCU)
of\emph{Prophantis octoguttalis} and \emph{P. adusta}

The concatenated lengths of the 13 PCGs of \emph{P. octoguttalis} and
\emph{P. adusta} were 11,196 bp and 11,219 bp, encoding 3732 and 3739
amino acids, respectively. Statistics on the relative synonymous codon
usage (RSCU) of \emph{P. octoguttalis}showed that the codons AUU (I),
UAA (*) and AAU (N) were used most frequently; these codons, AUU (I),
UUU (F) and AAA (K), were most frequently used in the mitogenome of
\emph{P. adusta.} The codons of amino acids with RSCU \textgreater{} 1
all contained A or U (Figure 2), and the preference of these codons
indirectly reflected the AT preference of the base.

3.3 rRNA genes and tRNA genes

In the mitogenomes of \emph{P. octoguttalis} and \emph{P. adusta} , two
rRNA genes were encoded by the minority strand, with concatenated
lengths of 2092 bp and 2077 bp, respectively. The \emph{rrnL} gene was
located between the \emph{trnL1} and \emph{trnV} genes, which were 1355
bp and 1341 bp long, respectively; the \emph{rrnS} gene was located
between the\emph{trnV} gene and the A+T-enriched regions, which were 737
bp and 736 bp long, respectively.

In the mitogenomes of these two species, there were 22 tRNA genes with
concatenated lengths of 1468 bp and 1481 bp, respectively. A total of 14
genes (\emph{trnM} , \emph{trnI} , \emph{trnW} , \emph{trnL2} ,
\emph{trnK} ,\emph{trnD} , \emph{trnG} , \emph{trnA} , \emph{trnR} ,
\emph{trnN} ,\emph{trnS1} , \emph{trnE} , \emph{trnT} , and \emph{trnS2}
) were encoded by the majority chain, and the remaining eight genes
(\emph{trnQ} ,\emph{trnC} , \emph{trnY} , \emph{trnF} , \emph{trnH} ,
\emph{trnP} ,\emph{trnL1} , and \emph{trnV} ) were encoded by the
minority chain, with the length of each gene ranging from 64--71 bp.
Except for \emph{trnS1}(AGN), which lacked the DHU arm, the secondary
structures of the remaining 21 tRNAs folded into a typical clover-leaf
structure (Figure 3). There were G-U and U-U base mismatches in the tRNA
genes, which mostly occurred in the DHU, AA acceptor, and anticodon
arms.

The AT content of the RNA gene of these two species was more than 80\%,
showing an obvious AT bias. As for base skew, both species showed a
slight A skew and an obvious C skew.

\textbf{Figure 3.} Secondary structure of tRNA of \emph{Prophantis
octoguttalis} and \emph{P. adusta}

3.4 Non-coding regions

The mitogenome of \emph{P. octoguttalis} had eight gene overlaps
totaling 24 bp, with a maximum overlap length of 8 bp between the
\emph{trnW} and\emph{trnC} genes, and 15 gene spacings totaling 172 bp,
with a maximum spacing length of 45 bp between the \emph{trnQ} and
\emph{nad2} genes. The mitogenome of \emph{P. adusta} had five gene
overlaps totaling 21 bp, with a maximum overlap length of 8 bp between
the \emph{trnW} and\emph{trnC} genes, and 18 gene spacings totaling 240
bp, with a maximum spacing length of 54 bp between the \emph{trnS1} and
\emph{trnE} genes.

The control regions of the mitogenomes of these two species were located
between the \emph{rrnS} and \emph{trnM} genes, with full lengths of 327
bp and 735 bp, respectively. Both sequences showed a clear AT bias, with
an AT content of 96.0\% and 96.7\%, respectively, which was
significantly higher than that of GC. The AT skew and GC skew of both
sequences were negative, showing a slight T skew and an obvious C skew.

3.5 Phylogenetic relationships

The mitogenomes of 76 Lepidoptera species were used in this study,
including four subfamilies of Pyralidae and eight subfamilies of
Crambidae, including Trichaeini, with \emph{Helicoverpa
armigera}(Hubner, 1805) (Noctuidae) and \emph{Bombyx mori} (Bombycidae)
as outgroups. Phylogenetic trees of Pyraloidea were reconstructed using
ML and BI analyses based on four datasets: PCG123, PCG123R, PCG123RT,
and PCG12. The phylogenetic trees obtained showed almost identical
topological structures, and the monophyly of both Pyralidae and
Crambidae was strongly supported (BS=100, PP=1). The topological
structure of the phylogenetic tree with PCG123RT was different from that
of the other three datasets; for brevity, only two phylogenetic
hypotheses (PCG123 and PCG123RT) were presented here (Figures 4, 5).

In Pyralidae, 25 species were divided into four major branches, and the
relationships of the four subfamilies could be expressed as Galleriinae
+ ((Epipaschiinae+ Pyralinae) + Phycitinae), which was consistent with
previous results based on mitochondrial genomic data analysis (Yang et
al., 2018a; Zhang et al., 2020; Liu et al., 2021; Qi et al., 2021).
Morphologically, \emph{Orybina} Snellen, 1895 is considered to be a
genus of Pyralinae. However, all of our phylogenetic trees showed
that\emph{O. plangonalis} (Walker, 1859) and \emph{O. regalis} Leech,
1889 were consistently located in the basal branches of the Pyralidae
clade, rendering Pyralinae paraphyletic. The monophyly of \emph{Orybina}
was very strongly supported (BS=100, PP=1), which was consistent with
the results of Liu et al. (2021).

Interestingly, \emph{Orthaga} Walker, 1859 is recognized morphologically
as a genus of Epipaschiinae, but \emph{O.} \emph{olivacea} (Warren,
1891) grouped with species of Pyralinae in both phylogenetic trees in
this study, and \emph{O. euadrusalis} Walker, 1859 was placed
with\emph{Lista haraldusalis} (Walker, 1858) to form the Epipaschiinae
branch. The phylogenetic analysis of Yang et al. (2020) inferred
that\emph{O.} \emph{olivacea} is more closely related to
\emph{Hypsopygia regina} (Butler, 1879) (Pyralinae), which is consistent
with the affinities shown in our phylogenetic trees. Therefore, the
monophyly of\emph{Orthaga} has not been well established. Additional
samples of this genus, with 49 species worldwide, are necessary for
further investigations to explore its monophyly and higher
relationships.

\textbf{Figure 4.} Phylogenetic tree constructed by BI analyses based on
the PCG123 dataset. Posterior probability (PP) values was shown on the
nodes

The Crambidae was divided into two major sister lineages, one consisting
of Pyraustinae and Spilomelinae, forming the ``PS clade,'' and the other
consisting of the remaining subfamilies that form the ``ono-PS clade,''
which was first defined by Regier et al. (2012). The ``ono-PS clade''
consists of the ``OG clade'' and ``CAMMSS clade,'' and our phylogenetic
trees showed that (Glaphyriinae + Odontiinae) aggregate into a branch
with high support to form the ``OG clade,'' and that (Schoenobiinae +
Acentropinae) + (Scopariinae + Crambinae) aggregate into a ``CAMMSS
clade'' with high support, which was compatible with previous studies
that combined mitochondrial and nuclear genes for analysis. Notably, the
ML and BI trees constructed based on PCG123RT (Figure 5) showed
differences in the topological structures of the ``CAMMSS clade'':
Scopariinae + (Crambinae + (Schoenobiinae + Acentropinae)), with strong
support. This difference was also evident in the topology of the ML tree
constructed by Liu et al. (2021) based on PCG123RT. In the present
taxonomic system, Crambidae consists of 15 subfamilies, containing more
than 10,000 species, and the mitochondrial genome data and nuclear genes
of more species should be used to infer the phylogenetic relationships
within this family.

\textbf{Figure 5.} Phylogenetic tree constructed by ML and BI analyses
based on the PCG123RT dataset. Bootstrap support (BS) and posterior
probability (PP) values were shown on the nodes

In Spilomelinae, \emph{P. octoguttalis} and \emph{P. adusta} aggregated
into a branch with high support (BS=100, PP=1) to form the Trichaeini,
which formed a sister-group relationship with Nomophilini. The
phylogenetic analysis by Mally et al. (2019) identified Trichaeini +
(Steniini + Nomophilini) in a tree constructed using COI, CAD, EF-1a,
GAPDH, IDH, and RpS5. Our results do not conflict with theirs.
Trichaeini and Nomophilini constituted a sister-group relationship in
the phylogenetic analysis of Japanese Pyraustinae and Spilomelinae by
Matsui et al. (2022), which was consistent with the results of the
present study. All phylogenetic trees based on the different datasets
showed satisfactorily high support values, confirming the monophyly and
position of Trichaeini.

\textbf{4 Conclusions}

In this study, we reported the complete mitogenomes of
two\emph{Prophantis} species, \emph{P. octoguttalis} and \emph{P.
adusta} , belonging to the tribe Trichaeini, for the first time, and
analyzed their gene size and arrangement, base composition, codon usage,
and tRNA secondary structure, etc., which were highly consistent with
those of other previously studied species of Spilomelinae. The two
mitogenomes were typical of lepidopteran insects. Combined with the
published mitogenome sequences of Pyraloidea, the results of the
phylogenetic analyses reconstructed on different datasets indicated that
Trichaeini was a monophyletic group, sister to Nomophilini. The
phylogenetic relationships within Pyraloidea were in general agreement
with previous studies, whereas the affinities in the ``CAMMSS clade''
were still unclear and require further study. Therefore, it is necessary
to improve sample coverage and combine different molecular markers, such
as nuclear genes with morphological characters, to further explore the
phylogenetic relationships among the pyraloid subfamilies.

\textbf{Acknowledgments}

We would like to thank Miss Yao Sheng and Miss Ruonan Xu (Laboratory of
Lepidoptera Systematics, Southwest University, Chongqing, China) for
their help with the sample collection. We also give our thanks to
Charles Lois for revising the manuscript.

\textbf{Conflicts of Interest}

All authors declare no conflicts of interest.

\textbf{Author contributions}

\textbf{Ci Tang} : Conceptualization (equal); Software (lead); Formal
Analysis (lead); Methodology (lead); Data Curation (equal);
Writing-original draft (lead); Writing-review \& editing (equal).

\textbf{Xicui Du} : Conceptualization (equal); Data Curation (equal);
Funding acquisition (lead); Project administration (lead); Resource
(lead); Supervision (lead); Writing-review \& editing (equal).

\textbf{Data Availability Statement}

GenBank accession number: \emph{Prophantis octoguttalis} (OP559507)
and\emph{P. adusta} (OP559508).

\textbf{Orcid}

\emph{Xicui Du} https://orcid.org/0000-0002-7796-7303

\textbf{References}

Boore, J. L. 1999. Animal mitochondrial genomes. \emph{Nucleic Acids
Research} , 27(8): 1767--1780. https://doi.org/10.1093/nar/27.8.1767

Boore, J. L. 2006. The use of genome-level characters for phylogenetic
reconstruction. \emph{Trends in Ecology \& Evolution} , 21(8): 439--446.
https://doi.org/10.1016/j.tree.2006.05.009

Cameron, S. L. 2014. Insect mitochondrial genomics: implications for
evolution and phylogeny. \emph{Annual Review of Entomology} , 59(6):
95--117. https://doi.org/10.1146/annurev-ento-011613-162007

Cao, S. S., \& Du, Y. Z. 2014. Characterization of the complete
mitochondrial genome of \emph{Chilo auricilius} and comparison with
three other rice stem borers. \emph{Gene} , 548(2): 270--276.
https://doi.org/10.1016/j.gene.2014.07.045

Cao, S. S., Yu, W. W., Meng, S., \& Zhou, D. Y. 2014. Characterization
of the complete mitochondrial genome of \emph{Tryporyza incertulas} , in
comparison with seven other Pyraloidea moths. \emph{Gene} , 533(1):
356--365. https://doi.org/10.1016/j.gene.2013.07.072

Chai, H. N., Du, Y. Z., \& Zhai, B. P. 2012. Characterization of the
complete mitochondrial genomes of \emph{Cnaphalocrocis medinalis}
and\emph{Chilo suppressalis} (Lepidoptera: Pyralidae).
\emph{International journal of biological sciences} , 8(4): 561.
https://doi: 10.7150/ijbs.3540

Chang, Z., \& Shen, Q. Q. 2016. The complete mitochondrial genome of the
navel orangeworm \emph{Amyelois transitella} (Insecta: Lepidoptera:
Pyralidae). \emph{Mitochondrial DNA Part A} , 27(6): 4561--4562.
https://doi.org/10.3109/19401736.2015.1101564

Chen, S., Li, F. H., Lan, X. E., \& You, P. 2016. The complete
mitochondrial genome of \emph{Pycnarmon lactiferalis} (Lepidoptera:
Crambidae). \emph{Mitochondrial DNA Part B} , 1(1): 638--639.
https://doi.org/10.1080/23802359.2016.1214551

Donath, A., Juhling, F., Al-Arab, M., Bernhart, S. H., Reinhardt, F.,
Stadler, P. F., Middendorf, M., \& Bernt, M. 2019. Improved annotation
of protein-coding genes boundaries in metazoan mitochondrial
genomes.\emph{Nucleic acids research} , 47(20): 10543--10552.
https://doi.org/10.1093/nar/gkz833

Fisher, K. E., Bradbury, S. P., \& Coates, B. S. 2020. Prediction of
mitochondrial genome-wide variation through sequencing of
mitochondrion-enriched extracts. \emph{Scientific reports} , 10(1):
1--13. https://doi.org/10.1038/s41598-020-76088-0

Gschloessl, B., Audiot, P., Nidelet, S., Kergoat, G. J., \& Streiff, R.
2020. Complete mitogenome data from a European specimen of\emph{Ostrinia
scapulalis} (Walker, 1859) (Lepidoptera, Pyraloidea, Crambidae,
Pyraustinae). \emph{Data in brief} , 33: 106427.
https://doi.org/10.1016/j.dib.2020.106427

He, S. L., Zou, Y., Zhang, L. F., Ma, W. Q., Zhang, X. Y., \& Yue, B. S.
2015. The complete mitochondrial genome of the beet
webworm,\emph{Spoladea recurvalis} (Lepidoptera: Crambidae) and its
phylogenetic implications. \emph{PloS one} , 10(6): e0129355.
https://doi.org/10.1371/journal.pone.0129355

Hu, Z. W., Zhong, L. K., Han, C., He, H. L., Liu, J. F., \& Yang, M. F.
2021. The complete mitochondrial genome sequence of \emph{Aglossa
dimidiata} (Haworth, 1809) (Lepidoptera: Pyralidae). \emph{Mitochondrial
DNA Part B} , 6(10): 2967--2968.
https://doi.org/10.1080/23802359.2021.1974965

Jeong, N. R., Kim, M. J., Park, J. S., Jeong, S. Y., \& Kim, I. 2021.
Complete mitochondrial genomes of \emph{Conogethes punctiferalis}
and\emph{C. pinicolalis} (Lepidoptera: Crambidae): Genomic comparison
and phylogenetic inference in Pyraloidea. \emph{Journal of Asia-Pacific
Entomology} , 24(4): 1179--1186.
https://doi.org/10.1016/j.aspen.2021.10.014

Johns, D. R. 1995. Mitochondrial DNA and disease. \emph{New England
journal of medicine} , 333(10): 638--644.
https://doi.org/10.1056/nejm199509073331007

Kong, W., \& Yang, J. 2016. The complete mitochondrial genome
of\emph{Diaphania pyloalis} Walker (Lepidoptera:
Crambidae).\emph{Mitochondrial DNA Part A} , 27(6): 4044--4045.
https://doi.org/10.3109/19401736.2014.1003836

Lanfear, R., Frandsen, P. B., Wright, A. M., Senfeld, T., \& Calcott, B.
2017. PartitionFinder 2: new methods for selecting partitioned models of
evolution for molecular and morphological phylogenetic
analyses.\emph{Molecular biology and evolution} , 34(3): 772--773.
https://doi.org/10.1093/molbev/msw260

Leger, T., Mally, R., Neinhuis, C., \& Nuss, M. 2021. Refining the
phylogeny of Crambidae with complete sampling of subfamilies
(Lepidoptera, Pyraloidea). \emph{Zoologica Scripta} , 50(1): 84--99.
https://doi.org/10.1111/zsc.12452

Li, W., Zhang, X., Fan, Z., Yue, B., Huang, F., King, E., \& Ran, J.
2011. Structural characteristics and phylogenetic analysis of the
mitochondrial genome of the sugarcane borer, \emph{Diatraea
saccharalis}(Lepidoptera: Crambidae). \emph{DNA and cell biology} ,
30(1): 3--8. https://doi.org/10.1089/dna.2010.1058

Li, C., Li, L., Ren, Y., Lu, Z., Song, Y., Liu, L., Lv, S. H., Yu, Y.,
\& Men, X. 2020. Characterization of the complete mitochondrial genome
of Asia Corn Borer, \emph{Ostrinia furnacalis} (Lepidoptera:
Crambidae).\emph{Mitochondrial DNA Part B} , 5(1): 936--937.
https://doi.org/10.1080/23802359.2020.1718025

Liu, Q. N., Chai, X. Y., Bian, D. D., Zhou, C. L., \& Tang, B. P. 2016.
The complete mitochondrial genome of \emph{Plodia
interpunctella}(Lepidoptera: Pyralidae) and comparison with other
Pyraloidea insects.\emph{Genome} , 59(1): 37--49.
https://doi.org/10.1139/gen-2015-0079

Liu, Q. Y., Jiang, X. H., Hou, X. H., Yang, H., \& Chen, W. L. 2018. The
mitochondrial genome of \emph{Ephestia elutella} (Insecta: Lepidoptera:
Pyralidae). \emph{Mitochondrial DNA Part B} , 3(1): 189--190.
https://doi.org/10.1080/23802359.2018.1436993

Liu, X., Qi, M., Xu, H., Wu, Z., Hu, L., Yang, M., \& Li, H. 2021. Nine
Mitochondrial Genomes of the Pyraloidea and Their Phylogenetic
Implications (Lepidoptera Pyraloidea). \emph{Insects} , 12(11): 1039.
https://doi.org/10.3390/insects12111039

Living Prairie Mitogenomics Consortium. 2017. The complete mitochondrial
genome of the lesser aspen webworm moth \emph{Meroptera
pravella}(Insecta: Lepidoptera: Pyralidae). \emph{Mitochondrial DNA Part
B} , 2(1): 344--346. https://doi.org/10.1080/23802359.2017.1334525

Ma, H. F., Zheng, X. X., Peng, M. H., Bian, H. X., Chen, M. M., Liu, Y.
Q., Jiang, X. F., \& Qin, L. 2016. Complete mitochondrial genome of the
meadow moth, \emph{Loxostege sticticalis} (Lepidoptera: Pyraloidea:
Crambidae), compared to other Pyraloidea moths. \emph{Journal of
Asia-Pacific Entomology} , 19: 697--706.
https://doi.org/10.1016/j.aspen.2016.05.011

Mao, X., Li, H. X., \& Yang, M. F. 2019. The complete mitochondrial
genome sequences of two insect-tea producers in Pyralidae (Lepidoptera)
from South China: \emph{Pyralis farinalis} and \emph{Orthopygia
glaucinalis} . \emph{Mitochondrial DNA Part B} , 4(2): 3850--3851.
https://doi.org/10.1080/23802359.2019.1687030

Mally, R., Hayden, J. E., Neinhuis, C., Jordal, B. H., \& Nuss, M. 2019.
The phylogenetic systematics of Spilomelinae and Pyraustinae
(Lepidoptera: Pyraloidea: Crambidae) inferred from DNA and
morphology.\emph{Senckenberg} , 77(1): 141--204.
https://doi.org/10.26049/asp77-1-2019-07

Matsui, Y., Mally, R., Kohama, S., Aoki, I., Azuma, M., \& Naka, H.
2022. Molecular phylogenetics and tribal classification of Japanese
Pyraustinae and Spilomelinae (Lepidoptera: Crambidae). \emph{Insect
Systematics \& Evolution} , 1(aop): 1--30.
https://doi.org/10.1163/1876312X-bja10037

Minh, B. Q., Nguyen, M. A. T., \& von Haeseler, A. 2013. Ultrafast
approximation for phylogenetic bootstrap. \emph{Molecular biology and
evolution} , 30(5): 1188--1195. https://doi.org/10.1093/molbev/mst024

Munroe, E. \& Solis, M.A. 1999. The Pyraloidea. Lepidoptera, Moths and
Butterflies, Vol. 1, Arthropoda, Insecta, Vol. 4, Part 35,\emph{Handbook
of Zoology} (ed. by N. Kristensen), pp. 233--256, 491 pp. Walter de
Gruyter \& Co, Berlin.

Nguyen, L. T., Schmidt, H. A., Von Haeseler, A., \& Minh, B. Q. 2015.
IQ-TREE: a fast and effective stochastic algorithm for estimating
maximum-likelihood phylogenies. \emph{Molecular biology and evolution} ,
32(1): 268--274. https://doi.org/10.1093/molbev/msu300

Nuss, M., Landry, B., Mally, R., Vegliante, F., Trankner, A., Bauer, F.,
Hayden, J., Segerer, A., Schouten, R., Li, H., Trofimova, T., Solis, M.
A., De Prins, J., \& Speidel, W. (2003--2020). \emph{Global information
system on Pyraloidea} . Retrieved from http://www.pyral oidea.org

Park, J. S., Kim, M. J., Kim, S. S., \& Kim, I. 2014. Complete
mitochondrial genome of an aquatic moth, \emph{Elophila
interruptalis}(Lepidoptera: Crambidae). \emph{Mitochondrial DNA} ,
25(4): 275--277. https://doi.org/10.3109/19401736.2013.800504

Park, J. S., Kim, M. J., Ahn, S. J., \& Kim, I. 2015. Complete
mitochondrial genome of the grass moth \emph{Glyphodes
quadrimaculalis}(Lepidoptera: Crambidae). \emph{Mitochondrial DNA} ,
26(2): 247--249. https://doi.org/10.3109/19401736.2013.823183

Park, Y. J., Park, C. E., Hong, S. J., Jung, B. K., Ibal, J. C., Park,
G. S., \& Shin, J. H. 2017. The complete mitochondrial genome sequence
of the greater wax moth \emph{Galleria mellonella} (Insecta,
Lepidoptera, Pyralidae): sequence and phylogenetic analysis comparison
based on whole mitogenome. \emph{Mitochondrial DNA Part B} , 2(2):
714--715. https://doi.org/10.1080/23802359.2017.1390418

Perna, N. T., \& Kocher, T. D. 1995. Patterns of nucleotide composition
at fourfold degenerate sites of animal mitochondrial
genomes.\emph{Journal of molecular evolution} , 41(3): 353--358.
https://doi.org/10.1007/BF00186547

Qi, M., Zhao, H., Yu, F., Zhang, A., \& Li, H. 2021. The first
mitogenomes of the subfamily Odontiinae (Lepidoptera, Crambidae) and
phylogenetic analysis of Pyraloidea. \emph{Insects} , 12(6): 486.
https://doi.org/10.3390/insects12060486

Que, S., Yu, A., Tu, Y., Xiong, C., \& Liu, X. 2019. Complete
mitochondrial genome and phylogenetic analysis of \emph{Diaphania
perspectalis} . \emph{Mitochondrial DNA Part B} , 4(1): 933--934.
https://doi.org/10.1080/23802359.2019.1568213

Regier, J. C., Mitter, C., Solis, M. A., Hayden, J. E., Landry, B.,
Nuss, M., Simonsen, T. J., Yen, S. H., Zwick, A., \& Cummings, M. P.
2012. A molecular phylogeny for the pyraloid moths (Lepidoptera:
Pyraloidea) and its implications for higher level
classification.\emph{Systematic Entomology} , 37(4): 635--656.
https://doi.org/10.1111/j.1365-3113.2012.00641.x

Roh, S. J., Jeon, J. H., Kim, D. S., \& Byun, B. K. 2020. The complete
mitochondrial genome of unique snout moth, \emph{Cathayia
obliquella}(Pyralidae: Galleriinae) with its phylogenetic implications
and a redescription of external morphology. \emph{Journal of
Asia-Pacific Biodiversity} , 13(4): 613--624.
https://doi.org/10.1016/j.japb.2020.09.007

Suzuki, H., Nunome, M., Kinoshita, G., Aplin, K. P., Vogel, P., Kryukov,
A. P., Jin, M. L., Han, S. H., I Maryanto., K Tsuchiya., H Ikeda., T
Shiroishi., H Yonekawa \& Moriwaki, K. 2013. Evolutionary and dispersal
history of Eurasian house mice Mus musculus clarified by more extensive
geographic sampling of mitochondrial DNA. \emph{Heredity} , 111(5):
375--390. https://doi.org/10.1038/hdy.2013.60

Taanman, J. W. 1999. The mitochondrial genome: structure, transcription,
translation and replication. \emph{Biochimica et Biophysica Acta} ,
1410(2): 103--123. https://doi.org/10.1016/S0005-2728(98)00161-3

Tang, M., Tan, M. H., Meng, G. L., Yang, S. Z., Su, X., Liu, S. L.,
Song, W. H., Li, Y. Y., Wu, Q., Zhang, A. B., \& Zhou, X. 2014.
Multiplex sequencing of pooled mitochondrial genomes-a crucial step
toward biodiversity analysis using mito-metagenomics. \emph{Nucleic
Acids Research} , 42(22): e166. https://doi.org/10.1093/nar/gku917

Timmermans, M. J., Lees, D. C., \& Simonsen, T. J. 2014. Towards a
mitogenomic phylogeny of Lepidoptera. \emph{Molecular phylogenetics and
evolution} , 79: 169--178. https://doi.org/10.1016/j.ympev.2014.05.031

Wang, J., Li, P., \& You, P. 2016. The complete mitochondrial genome
of\emph{Tyspanodes hypsalis} (Lepidoptera: Crambidae).
\emph{Mitochondrial DNA Part A} , 27(3): 1821--1822.
https://doi.org/10.3109/19401736.2014.971241

Wesley, M. B., Matthew, G. J. R., \& Wilson, A. C. 1979. Rapid evolution
of animal mitochondrial DNA. \emph{Proceedings of the national academy
of sciences} , 76(4): 1967--1971. https://doi.org/10.1073/pnas.76.4.1967

Wu, Q. L., Gong, Y. J., Shi, B. C., Gu, Y., \& Wei, S. J. 2013. The
complete mitochondrial genome of the yellow peach moth Dichocrocis
punctiferalis (Lepidoptera: Pyralidae). \emph{Mitochondrial DNA} ,
24(2), 105--107. https://doi.org/10.3109/19401736.2012.726621

Wu, Y. P., Li, J., Zhao, J. L., Su, T. J., Luo, A. R., Fan, R. J., Chen,
M. C., Wu, C. S., \& Zhu, C. D. 2012. The complete mitochondrial genome
of the rice moth, \emph{Corcyra cephalonica} . \emph{Journal of insect
science} , 12(1): 72. https://doi.org/10.1673/031.012.7201

Wu, Y. P., Lu, J. J., Yang, J., \& Fan, R. J. 2020. Complete
mitochondrial genome of \emph{Dioryctria yiai} (Lepidoptera:
Pyralidae).\emph{Mitochondrial DNA Part B} , 5(1): 1062--1064.
https://doi.org/10.1080/23802359.2020.1721352

Wu, Y., Liu, X., Zhang, Y., Fang, H., Lu, J., \& Wang, J. 2022.
Characterization of four mitochondrial genomes of Crambidae
(Lepidoptera, Pyraloidea) and phylogenetic implications. \emph{Archives
of Insect Biochemistry and Physiology} , e21914.
https://doi.org/10.1002/arch.21914

Yang, M. L., Feng, S. Q., Cao, Y., Han, X., Xiong, R. C., \& Li, Z. H.
2017. The complete mitochondrial genome of the pear pyralid
moth,\emph{Euzophera pyriella} Yang. \emph{Mitochondrial DNA Part B} ,
2(1): 275--276. https://doi.org/10.1080/23802359.2017.1325338

Yang, M. S., Song, L., Mao, J. H., Shi, Y. X., Wu, C. J., Zhang, Y. X.,
Huang, L., Peng, W. F., \& Liu, X. M. 2018a. Complete mitochondrial
genome of the soybean leaffolder, \emph{Omiodes indicata} (Lepidoptera:
Pyraloidea: Crambidae), and phylogenetic analysis for
Pyraloidea.\emph{Biological Macromolecules} , 115: 53--60.
https://doi.org/10.1016/j.ijbiomac.2018.03.041

Yang, M. S., Shi, S., Dai, P., Song, L., \& Liu, X. M. 2018b. Complete
mitochondrial genome of \emph{Palpita hypohomalia} (Lepidoptera:
Pyraloidea: Crambidae) and its phylogenetic implications. \emph{European
Journal of Entomology} , 115: 708--717.
https://doi.org/10.14411/eje.2018.070

Yang, L., Dai, J., Gao, Q., Yuan, G., Liu, J., Sun, Y., Sun, Y. X.,
Wang, L., Qian, C., Zhu, B. J., Liu, C. L., \& Wei, G. 2020.
Characterization of the complete mitochondrial genome of \emph{Orthaga
olivacea} Warre (Lepidoptera Pyralidae) and comparison with other
Lepidopteran insects. \emph{PloS one} , 15(3): e0227831.
https://doi.org/10.1371/journal.pone.0227831

Ye, F., Shi, Y., Xing, L., Yu, H., \& You, P. 2013. The complete
mitochondrial genome of \emph{Paracymoriza prodigalis} (Leech, 1889)
(Lepidoptera), with a preliminary phylogenetic analysis of
Pyraloidea.\emph{Aquatic Insects} , 35(3--4): 71--88.
https://doi.org/10.1080/01650424.2014.948456

Ye, F., Yu, H. L., Li, P. F., \& You, P. 2015. The complete
mitochondrial genome of \emph{Lista haraldusalis} (Lepidoptera:
Pyralidae). \emph{Mitochondrial DNA} , 26(6): 853--854.
https://doi.org/10.3109/19401736.2013.861427

Ye, F., \& You, P. 2016. The complete mitochondrial genome
of\emph{Paracymoriza distinctalis} (Lepidoptera:
Crambidae).\emph{Mitochondrial DNA Part A} , 27(1): 28--29.
https://doi.org/10.3109/19401736.2013.869678

Yin, Y., Qu, F., Yang, Z., Zhang, X., \& Yue, B. 2014. Structural
characteristics and phylogenetic analysis of the mitochondrial genome of
the rice leafroller, \emph{Cnaphalocrocis medinalis} (Lepidoptera:
Crambidae). \emph{Molecular biology reports} , 41(2): 1109--1116.
https://doi.org/10.1007/s11033-013-2957-9

Yin, J., Hong, G. Y., Wang, A. M., Cao, Y. Z., \& Wei, Z. J. 2010.
Mitochondrial genome of the cotton bollworm \emph{Helicoverpa
armigera}(Lepidoptera: Noctuidae) and comparison with other
Lepidopterans.\emph{Mitochondrial DNA} , 21(5): 160--169.
https://doi.org/10.3109/19401736.2010.503242

Zhang, K. J., Rong, X., Lv, Z. P., Liu, L., Li, G. Y., \& Liu, H. 2020.
Comparative Mitochondrial Analysis of \emph{Cnaphalocrocis
exigua}(Lepidoptera: Crambidae) and Its Close Relative \emph{C.
medinalis} .\emph{Journal of Insect Science} , 20(6): 24.
https://doi.org/10.1093/jisesa/ieaa112

Zhao, J., Sun, Y., Xiao, L., Tan, Y., \& Bai, L. 2016. Complete
mitochondrial genome of cotton leaf roller \emph{Haritalodes
derogata}(Lepidoptera: Crambidae). \emph{Mitochondrial DNA Part A} ,
27(4): 2833--2834. https://doi.org/10.3109/19401736.2015.1053115

Zhou, N., Dong, Y., Qiao, P., \& Yang, Z. 2020. Complete mitogenomic
structure and phylogenetic implications of the genus
\emph{Ostrinia}(Lepidoptera: Crambidae). \emph{Insects} , 11(4): 232.
https://doi.org/10.3390/insects11040232

Zhu, W., Yan, J., Song, J., \& You, P. 2018. The first mitochondrial
genomes for Pyralinae (Pyralidae) and Glaphyriinae (Crambidae), with
phylogenetic implications of Pyraloidea. \emph{PLoS One} , 13(3):
e0194672. https://doi.org/10.1371/journal.pone.0194672

Zou, Y., Ma, W., Zhang, L., He, S., Zhang, X., \& Tao, Z. 2016. The
complete mitochondrial genome of the bean pod borer, \emph{Maruca
testulalis} (Lepidoptera: Crambidae: Spilomelinae). \emph{Mitochondrial
DNA Part A} , 27(1): 740--741.
https://doi.org/10.3109/19401736.2014.913167

\textbf{Figures} \textbf{legends}

\textbf{Figure 1.} Visualization of the mitochondrial genomes
of\emph{Prophantis octoguttalis} and \emph{P. adusta}

\textbf{Figure 2.} Relative synonymous codon usage (RSCU)
of\emph{Prophantis octoguttalis} and \emph{P. adusta}

\textbf{Figure 3.} Secondary structure of tRNA of \emph{Prophantis
octoguttalis} and \emph{P. adusta}

\textbf{Figure 4.} Phylogenetic tree constructed by BI analyses based on
the PCG123 dataset. Posterior probability (PP) values was shown on the
nodes

\textbf{Figure 5.} Phylogenetic tree constructed by ML and BI analyses
based on the PCG123RT dataset. Bootstrap support (BS) and posterior
probability (PP) values were shown on the nodes

\textbf{Tables}

\textbf{Table 1.} The mitochondrial genome sequences used in the
phylogenetic analyses.

Table 1. should go to 2.3 Phylogenetic analysis.\selectlanguage{english}
\begin{longtable}[]{@{}llllll@{}}
\toprule
Family & Subfamily & Species & GenBank ID & References &
References\tabularnewline
\midrule
\endhead
Bombycidae & Bombycinae & \emph{Bombyx mori} & NC002355 & Direct
Submission & Direct Submission\tabularnewline
Crambidae & Acentropinae & \emph{Cataclysta lemnata} & MT410858 & Direct
Submission & Direct Submission\tabularnewline
& & \emph{Elophila interruptalis} & KC894961 & Park et al., 2014 & Park
et al., 2014\tabularnewline
& & \emph{Parapoynx crisonalis} & KT443883 & Direct Submission & Direct
Submission\tabularnewline
& & \emph{Paracymoriza distinctalis} & KF859965 & Ye and You, 2016 & Ye
and You, 2016\tabularnewline
& & \emph{Paracymoriza prodigalis} & JX144892 & Ye et al., 2013 & Ye et
al., 2013\tabularnewline
& Crambinae & \emph{Chilo auricilius} & KJ174087 & Cao and Du et al.,
2014 & Cao and Du et al., 2014\tabularnewline
& & \emph{Chilo sacchariphagus} & KU188518 & Direct Submission & Direct
Submission\tabularnewline
& & \emph{Chilo suppressalis} & JF339041 & Chai et al., 2012 & Chai et
al., 2012\tabularnewline
& & \emph{Diatraea saccharalis} & FJ240227 & Li et al., 2011 & Li et
al., 2011\tabularnewline
& & \emph{Pseudargyria interruptella} & KP071469 & Direct Submission &
Direct Submission\tabularnewline
& Glaphyriinae & \emph{Evergestis junctalis} & KP347976 & Direct
Submission & Direct Submission\tabularnewline
& Odontiinae & \emph{Dausara latiterminalis} & MW732137 & Qi et al.,
2021 & Qi et al., 2021\tabularnewline
& & \emph{Heortia vitessoides} & NC056800 & Qi et al., 2021 & Qi et al.,
2021\tabularnewline
& & \emph{Pseudonoorda nigropunctalis} & MW732139 & Qi et al., 2021 & Qi
et al., 2021\tabularnewline
& Pyraustinae & \emph{Loxostege aeruginalis} & MN635734 & Wu et al.,
2022 & Wu et al., 2022\tabularnewline
& & \emph{Loxostege sticticalis} & KR080490 & Ma et al., 2016 & Ma et
al., 2016\tabularnewline
& & \emph{Loxostege turbidalis} & MN646773 & Wu et al., 2022 & Wu et
al., 2022\tabularnewline
& & \emph{Ostrinia furnacalis} & NC056248 & Li et al., 2020 & Li et al.,
2020\tabularnewline
& & \emph{Ostrinia nubilalis} & NC054270 & Fisher et al., 2020 & Fisher
et al., 2020\tabularnewline
& & \emph{Ostrinia scapulalis} & MT801073 & Gschloessl et al., 2020 &
Gschloessl et al., 2020\tabularnewline
& & \emph{Ostrinia zealis} & NC048888 & Zhou et al., 2020 & Zhou et al.,
2020\tabularnewline
& & \emph{Pyrausta despicata} & MN956508 & Wu et al., 2022 & Wu et al.,
2022\tabularnewline
& Schoenobiinae & \emph{Scirpophaga incertulas} & NC031329 & Cao et al.,
2014 & Cao et al., 2014\tabularnewline
& Scopariinae & \emph{Eudonia angustea} & KJ508052 & Timmermans et al.,
2014 & Timmermans et al., 2014\tabularnewline
& Spilomelinae & \emph{Botyodes principalis} & MZ823351 & Liu et al.,
2021 & Liu et al., 2021\tabularnewline
& & \emph{Cnaphalocrocis medinalis} & JQ305693 & Yin et al., 2014 & Yin
et al., 2014\tabularnewline
& & \emph{Conogethes pinicolalis} & MT674993 & Jeong et al., 2021 &
Jeong et al., 2021\tabularnewline
& & \emph{Conogethes punctiferalis} & NC021389 & Wu et al., 2013 & Wu et
al., 2013\tabularnewline
& & \emph{Cydalima perspectalis} & MH602288 & Que et al., 2019 & Que et
al., 2019\tabularnewline
& & \emph{Glyphodes pyloalis} & NC025933 & Kong and Yang, 2016 & Kong
and Yang, 2016\tabularnewline
& & \emph{Glyphodes quadrimaculalis} & KF234079 & Park et al., 2015 &
Park et al., 2015\tabularnewline
& & \emph{Haritalodes derogata} & KR233479 & Zhao et al., 2016 & Zhao et
al., 2016\tabularnewline
& & \emph{Marasmia exigua} & MN877384 & Zhang et al., 2020 & Zhang et
al., 2020\tabularnewline
& & \emph{Maruca testulalis} & KJ623250 & Zou et al., 2016 & Zou et al.,
2016\tabularnewline
& & \emph{Maruca vitrata} & NC024099 & Direct Submission & Direct
Submission\tabularnewline
& & \emph{Nagiella inferior} & MF373813 & Direct Submission & Direct
Submission\tabularnewline
& & \emph{Nomophila noctuella} & KM244688 & Tang et al., 2014 & Tang et
al., 2014\tabularnewline
& & \emph{Omiodes indicata} & MG770232 & Yang et al., 2018a & Yang et
al., 2018a\tabularnewline
& & \emph{Palpita hypohomalia} & MG869628 & Yang et al., 2018b & Yang et
al., 2018b\tabularnewline
& & \emph{Palpita nigropunctalis} & KX150458 & Direct Submission &
Direct Submission\tabularnewline
& & \emph{Prophantis adusta} & & This study & This study\tabularnewline
& & \emph{Prophantis octoguttalis} & & This study & This
study\tabularnewline
& & \emph{Pycnarmon lactiferalis} & KX426346 & Chen et al., 2016 & Chen
et al., 2016\tabularnewline
& & \emph{Pycnarmon pantherata} & KX150459 & Direct Submission & Direct
Submission\tabularnewline
& & \emph{Sinomphisa plagialis} & MZ823346 & Liu et al., 2021 & Liu et
al., 2021\tabularnewline
& & \emph{Spoladea recurvalis} & KJ739310 & He et al., 2015 & He et al.,
2015\tabularnewline
& & \emph{Syllepte taiwanalis} & MZ823348 & Liu et al., 2021 & Liu et
al., 2021\tabularnewline
& & \emph{Tyspanodes hypsalis} & KM453724 & Wang et al., 2016 & Wang et
al., 2016\tabularnewline
& & \emph{Tyspanodes striata} & KP347977 & Direct Submission & Direct
Submission\tabularnewline
Noctuidae & Heliothinae & \emph{Helicoverpa armigera} & NC014668 & Yin
et al., 2010 & Yin et al., 2010\tabularnewline
Pyralidae & Epipaschiinae & \emph{Orthaga euadrusalis} & MZ823349 & Liu
et al., 2021 & Liu et al., 2021\tabularnewline
& & \emph{Orthaga olivacea} & MN078362 & Yang et al., 2020 & Yang et
al., 2020\tabularnewline
& & \emph{Lista haraldusalis} & KF709449 & Ye et al., 2015 & Ye et al.,
2015\tabularnewline
& Galleriinae & \emph{Cathayia obliquella} & MK550620 & Roh et al., 2020
& Roh et al., 2020\tabularnewline
& & \emph{Corcyra cephalonica} & HQ897685 & Wu et al., 2012 & Wu et al.,
2012\tabularnewline
& & \emph{Galleria mellonella} & KT750964 & Park et al., 2017 & Park et
al., 2017\tabularnewline
& & \emph{Paralipsa gularis} & MW135332 & Direct Submission & Direct
Submission\tabularnewline
& Phycitinae & \emph{Acrobasis inouei} & MZ823347 & Liu et al., 2021 &
Liu et al., 2021\tabularnewline
& & \emph{Amyelois transitella} & KT692987 & Chang and Shen, 2016 &
Chang and Shen, 2016\tabularnewline
& & \emph{Dioryctria rubella} & MZ823345 & Liu et al., 2021 & Liu et
al., 2021\tabularnewline
& & \emph{Dioryctria yiai} & MN658208 & Wu et al., 2020 & Wu et al.,
2020\tabularnewline
& & \emph{Dusungwua basinigra} & MZ902334 & Liu et al., 2021 & Liu et
al., 2021\tabularnewline
& & \emph{Ephestia elutella} & MG748858 & Liu et al., 2018 & Liu et al.,
2018\tabularnewline
& & \emph{Ephestia kuehniella} & KU877167 & Direct Submission & Direct
Submission\tabularnewline
& & \emph{Euzophera pyriella} & KY825744 & Yang et al., 2017 & Yang et
al., 2017\tabularnewline
& & \emph{Meroptera pravella} & MF073207 & Living Prairie Mitogenomics
Consortium, 2017 & Living Prairie Mitogenomics Consortium,
2017\tabularnewline
& & \emph{Plodia interpunctella} & MN619781 & Liu et al., 2016 & Liu et
al., 2016\tabularnewline
& Pyralinae & \emph{Aglossa dimidiata} & MW542312 & Hu et al., 2021 & Hu
et al., 2021\tabularnewline
& & \emph{Endotricha consocia} & MF568544 & Zhu et al., 2018
&\tabularnewline
& & \emph{Endotricha olivacealis} & MZ823344 & Liu et al., 2021
&\tabularnewline
& & \emph{Hypsopygia regina} & KP327714 & Direct Submission
&\tabularnewline
& & \emph{Orthopygia glaucinalis} & MN461479 & Mao et al., 2019
&\tabularnewline
& & \emph{Orybina plangonalis} & MF568543 & Zhu et al., 2018
&\tabularnewline
& & \emph{Orybina regalis} & MZ823350 & Liu et al., 2021
&\tabularnewline
& & \emph{Pyralis farinalis} & MN442120 & Mao et al., 2019
&\tabularnewline
\bottomrule
\end{longtable}

\textbf{Table 2.} Mitogenomic organization of \emph{Prophantis
octoguttalis} and \emph{P. adusta}

Table 2. should go to 3.1 Basic structure\selectlanguage{english}
\begin{longtable}[]{@{}lllllllllll@{}}
\toprule
\begin{minipage}[b]{0.09\columnwidth}\raggedright\strut
Gene\strut
\end{minipage} & \begin{minipage}[b]{0.09\columnwidth}\raggedright\strut
Strand\strut
\end{minipage} & \begin{minipage}[b]{0.09\columnwidth}\raggedright\strut
Position\strut
\end{minipage} & \begin{minipage}[b]{0.09\columnwidth}\raggedright\strut
Position\strut
\end{minipage} & \begin{minipage}[b]{0.09\columnwidth}\raggedright\strut
Size\strut
\end{minipage} & \begin{minipage}[b]{0.09\columnwidth}\raggedright\strut
Size\strut
\end{minipage} & \begin{minipage}[b]{0.09\columnwidth}\raggedright\strut
Intergenic nucleotides\strut
\end{minipage} & \begin{minipage}[b]{0.09\columnwidth}\raggedright\strut
Intergenic nucleotides\strut
\end{minipage} & \begin{minipage}[b]{0.09\columnwidth}\raggedright\strut
Start / Stop Codon\strut
\end{minipage} & \begin{minipage}[b]{0.09\columnwidth}\raggedright\strut
Start / Stop Codon\strut
\end{minipage} & \begin{minipage}[b]{0.09\columnwidth}\raggedright\strut
Anticodon\strut
\end{minipage}\tabularnewline
\midrule
\endhead
\begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
Po\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
Pa\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
Po\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
Pa\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
Po\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
Pa\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
Po\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
Pa\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\strut
\end{minipage}\tabularnewline
\begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\emph{trnM}\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
J\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
1-67\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
1-68\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
67\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
68\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
0\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
0\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
CAT\strut
\end{minipage}\tabularnewline
\begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\emph{trnI}\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
J\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
68-131\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
69-133\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
64\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
65\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
-3\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
-3\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
GAT\strut
\end{minipage}\tabularnewline
\begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\emph{trnQ}\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
N\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
129-197\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
131-199\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
69\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
69\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
45\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
46\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
TTG\strut
\end{minipage}\tabularnewline
\begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\emph{nad2}\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
J\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
243-1256\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
246-1259\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
1014\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
1014\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
13\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
11\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
ATT/TAA\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
ATT/TAA\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\strut
\end{minipage}\tabularnewline
\begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\emph{trnW}\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
J\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
1270-1337\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
1271-1338\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
68\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
68\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
-8\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
-8\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
TCA\strut
\end{minipage}\tabularnewline
\begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\emph{trnC}\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
N\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
1330-1394\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
1331-1400\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
65\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
70\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
19\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
20\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
GCA\strut
\end{minipage}\tabularnewline
\begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\emph{trnY}\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
N\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
1414-1482\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
1421-1487\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
69\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
67\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
8\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
15\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
GTA\strut
\end{minipage}\tabularnewline
\begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\emph{cox1}\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
J\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
1491-3021\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
1503-3033\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
1531\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
1531\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
0\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
0\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
CGA/T-\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
CGA/T-\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\strut
\end{minipage}\tabularnewline
\begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\emph{trnL2}\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
J\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
3022-3088\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
3034-3100\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
67\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
67\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
0\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
0\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
TAA\strut
\end{minipage}\tabularnewline
\begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\emph{cox2}\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
J\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
3089-3770\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
3101-3782\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
682\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
682\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
0\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
0\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
ATG/T-\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
ATG/T-\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\strut
\end{minipage}\tabularnewline
\begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\emph{trnK}\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
J\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
3771-3841\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
3783-3853\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
71\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
71\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
3\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
3\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
CTT\strut
\end{minipage}\tabularnewline
\begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\emph{trnD}\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
J\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
3845-3911\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
3857-3924\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
67\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
68\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
0\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
0\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
GTC\strut
\end{minipage}\tabularnewline
\begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\emph{atp8}\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
J\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
3912-4070\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
3925-4089\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
159\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
165\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
-7\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
-7\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
ATA/TAA\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
ATA/TAA\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\strut
\end{minipage}\tabularnewline
\begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\emph{atp6}\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
J\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
4064-4738\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
4083-4757\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
675\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
675\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
-1\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
8\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
ATG/TAA\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
ATG/TAA\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\strut
\end{minipage}\tabularnewline
\begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\emph{cox3}\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
J\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
4738-5526\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
4766-5554\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
789\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
789\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
2\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
2\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
ATG/TAA\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
ATG/TAA\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\strut
\end{minipage}\tabularnewline
\begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\emph{trnG}\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
J\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
5529-5593\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
5557-5621\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
65\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
65\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
0\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
0\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
TCC\strut
\end{minipage}\tabularnewline
\begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\emph{nad3}\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
J\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
5594-5947\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
5622-5975\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
354\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
354\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
-1\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
12\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
ATA/TAA\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
ATT/TAA\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\strut
\end{minipage}\tabularnewline
\begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\emph{trnA}\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
J\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
5947-6011\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
5988-6053\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
65\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
66\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
1\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
-1\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
TGC\strut
\end{minipage}\tabularnewline
\begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\emph{trnR}\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
J\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
6013-6076\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
6053-6118\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
64\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
66\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
4\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
14\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
TCG\strut
\end{minipage}\tabularnewline
\begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\emph{trnN}\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
J\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
6081-6145\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
6113-6198\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
65\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
66\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
7\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
9\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
GTT\strut
\end{minipage}\tabularnewline
\begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\emph{trnS1}\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
J\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
6153-6218\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
6208-6273\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
66\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
66\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
9\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
54\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
GCT\strut
\end{minipage}\tabularnewline
\begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\emph{trnE}\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
J\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
6228-6293\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
6328-6394\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
66\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
67\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
-2\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
-2\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
TTC\strut
\end{minipage}\tabularnewline
\begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\emph{trnF}\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
N\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
6292-6358\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
6393-6462\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
67\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
70\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
0\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
0\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
GAA\strut
\end{minipage}\tabularnewline
\begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\emph{nad5}\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
N\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
6359-8093\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
6463-8197\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
1735\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
1735\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
0\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
0\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
ATT/T-\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
ATT/T-\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\strut
\end{minipage}\tabularnewline
\begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\emph{trnH}\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
N\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
8094-8159\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
8198-8263\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
66\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
66\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
-1\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
13\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
GTG\strut
\end{minipage}\tabularnewline
\begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\emph{nad4}\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
N\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
8159-9499\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
8277-9617\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
1341\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
1341\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
0\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
0\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
ATG/TAA\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
ATG/TAA\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\strut
\end{minipage}\tabularnewline
\begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\emph{nad4l}\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
N\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
9500-9793\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
9618-9911\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
294\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
294\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
2\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
2\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
ATG/TAA\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
ATG/TAA\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\strut
\end{minipage}\tabularnewline
\begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\emph{trnT}\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
J\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
9796-9862\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
9914-9979\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
67\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
66\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
0\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
0\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
TGT\strut
\end{minipage}\tabularnewline
\begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\emph{trnP}\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
N\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
9863-9928\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
9980-10045\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
66\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
66\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
2\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
2\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
TGG\strut
\end{minipage}\tabularnewline
\begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\emph{nad6}\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
J\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
9931-10464\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
10048-10581\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
534\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
534\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
5\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
4\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
ATT/TAA\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
ATT/TAA\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\strut
\end{minipage}\tabularnewline
\begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\emph{cob}\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
J\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
10470-11618\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
10586-11734\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
1149\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
1149\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
-1\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
5\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
ATG/TAA\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
ATG/TAA\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\strut
\end{minipage}\tabularnewline
\begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\emph{trnS2}\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
J\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
11618-11682\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
11740-11806\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
65\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
67\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
18\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
19\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
TGA\strut
\end{minipage}\tabularnewline
\begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\emph{nad1}\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
N\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
11701-12639\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
11826-12764\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
939\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
939\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
0\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
1\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
ATG/TAA\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
ATG/TAA\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\strut
\end{minipage}\tabularnewline
\begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\emph{trnL1}\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
N\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
12640-12707\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
12766-12833\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
68\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
68\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
29\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
0\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
TAG\strut
\end{minipage}\tabularnewline
\begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\emph{rrnL}\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
N\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
12708-14062\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
12834-14174\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
1355\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
1341\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
0\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
0\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\strut
\end{minipage}\tabularnewline
\begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\emph{trnV}\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
N\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
14063-14133\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
14175-14243\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
71\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
69\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
0\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
0\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
TAC\strut
\end{minipage}\tabularnewline
\begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\emph{rrnS}\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
N\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
14134-14870\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
14244-14979\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
737\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
736\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
0\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
0\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\strut
\end{minipage}\tabularnewline
\begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
CR\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
14871-15197\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
14980-15714\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
327\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
735\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\strut
\end{minipage} & \begin{minipage}[t]{0.07\columnwidth}\raggedright\strut
\strut
\end{minipage}\tabularnewline
\bottomrule
\end{longtable}

\textbf{Table 3.} Nucleotide composition of \emph{Prophantis
octoguttalis} and \emph{P. adusta}

Table 3. should go to 3.1 Basic structure\selectlanguage{english}
\begin{longtable}[]{@{}lllllllllllllllll@{}}
\toprule
Regions & T & T & C & C & A & A & G & G & A+T & A+T & AT skew & AT skew
& GC skew & GC skew & Total(bp) & Total(bp)\tabularnewline
\midrule
\endhead
& Po & Pa & Po & Pa & Po & Pa & Po & Pa & Po & Pa & Po & Pa & Po & Pa &
Po & Pa\tabularnewline
Whole & 40.5 & 40.7 & 11.0 & 11.0 & 41.0 & 40.8 & 7.5 & 7.4 & 81.5 &
81.6 & 0.006 & 0.001 & -0.189 & -0.196 & 15197 & 15714\tabularnewline
PGCs & 39.8 & 39.7 & 11.6 & 12.1 & 40.5 & 39.9 & 8.2 & 8.4 & 80.3 & 79.6
& 0.01 & 0.003 & -0.173 & -0.181 & 11196 & 11219\tabularnewline
1st codon & 41.6 & 35.2 & 9.0 & 14.4 & 41.1 & 37.9 & 8.4 & 12.5 & 82.7 &
73.1 & -0.006 & 0.037 & -0.034 & -0.071 & 3732 & 3740\tabularnewline
2st codon & 36.8 & 42.5 & 14.6 & 13.4 & 38.1 & 37.3 & 10.5 & 6.7 & 74.9
& 79.8 & 0.017 & -0.065 & -0.163 & -0.333 & 3732 & 3740\tabularnewline
3st codon & 41.0 & 41.4 & 11.1 & 8.4 & 42.2 & 44.4 & 5.7 & 5.8 & 83.2 &
85.8 & 0.014 & 0.035 & -0.321 & -0.183 & 3732 & 3739\tabularnewline
rRNA & 42.4 & 43.3 & 10.1 & 9.7 & 42.5 & 42.0 & 5.0 & 5.0 & 84.9 & 85.3
& 0.001 & -0.015 & -0.338 & -0.32 & 2092 & 2077\tabularnewline
tRNA & 40.7 & 39.8 & 9.9 & 10.3 & 41.4 & 42.1 & 7.9 & 7.7 & 82.2 & 82.0
& 0.009 & 0.028 & -0.112 & -0.144 & 1468 & 1481\tabularnewline
RNAs & 41.7 & 41.9 & 10.0 & 10.0 & 42.1 & 42.0 & 6.2 & 6.1 & 83.8 & 83.9
& 0.005 & 0.001 & -0.235 & -0.242 & 3560 & 3558\tabularnewline
CR & 49.8 & 49.7 & 3.1 & 2.2 & 46.2 & 47.1 & 0.9 & 1.1 & 96.0 & 96.7 &
-0.038 & -0.027 & -0.55 & -0.333 & 327 & 735\tabularnewline
\bottomrule
\end{longtable}\selectlanguage{english}
\begin{figure}[H]
\begin{center}
\includegraphics[width=0.70\columnwidth]{figures/Figure-1/Figure-1}

\end{center}
\end{figure}\selectlanguage{english}
\begin{figure}[H]
\begin{center}
\includegraphics[width=0.70\columnwidth]{figures/Figure-2/Figure-2}

\end{center}
\end{figure}\selectlanguage{english}
\begin{figure}[H]
\begin{center}
\includegraphics[width=0.70\columnwidth]{figures/Figure-3/Figure-3}

\end{center}
\end{figure}\selectlanguage{english}
\begin{figure}[H]
\begin{center}
\includegraphics[width=0.70\columnwidth]{figures/Figure-4/Figure-4}

\end{center}
\end{figure}\selectlanguage{english}
\begin{figure}[H]
\begin{center}
\includegraphics[width=0.70\columnwidth]{figures/Figure-5/Figure-5}

\end{center}
\end{figure}

\selectlanguage{english}
\FloatBarrier
\end{document}

