Plasmids Characters Reference
PGAPZA Zeocinr, Expression vector with GAP promoter Invitrogen
Ppic3.5k Kana r, Expression vector Invitrogen
PGAP-Z PPISI-Z PGPM1-Z PMET3-Z PPGK1-Z Zeocinr, PGAPZA plasmid with mutated LoxP site and his4 gene Replacing the GAP promoter with the PISI promoter in the PGAP-Z plasmid Replacing the GAP promoter with the GPM1 promoter in the PGAP-Z plasmid Replacing the GAP promoter with the MET3 promoter in the PGAP-Z plasmid Replacing the GAP promoter with the PGK1 promoter in the PGAP-Z plasmid This study This study This study This study This study
PGAP-1 PGAP-2 PGAP-3 PGAP-4 PGAP-5 PPISI-1 PGPM1-1 PMET3-1 PPGK1-1 APRT-1 Pcas9-PG1-sg Pcas9-GPD1-sg PGAP-Z carrying gene zwf1 PGAP-Z carrying gene sol3 PGAP-Z carrying gene gnd2 PGAP-Z carrying gene rpe1 PGAP-Z carrying gene cPOS5 PPISI-Z carrying gene cPOS5 PGPM1-Z carrying gene cPOS5 PMET3-Z carrying gene cPOS5 PPGK1-Z carrying gene cPOS5 PGAP-Z carrying gene aprt Ppic3.5k plasmid with PTHX1 promoter, cas9 and PG1-sgRNA. And the his4 gene was replaced with the ARS gene of P. pastoris Ppic3.5k plasmid with PTHX1 promoter, cas9 and GPD1-sgRNA. And the his4 gene was replaced with the ARS gene of P. pastoris This study This study This study This study This study This study This study This study This study This study This study This study