Quantification of intracellular NADH/NAD+,
NADPH/NADP+ and ATP
Strains were cultivated in YPD medium for 24 h or 72 h, and cells were
harvested by centrifugation at 4°C for 30 min at 10,000×g and
re-suspended in cold PBS buffer to OD600=10. The
intracellular NADH/NAD and NADPH/NADP were extracted according to the
previously described by Faijes et al. 23, and the
intracellular ATP were extracted according to Ni et al.’s
reports.24 Their concentration were measured using
NADH/NAD+ Quantification Colorimeteric Kit,
NADPH/NADP+ Quantification Colorimeteric Kit and ATP
Colorimetric/Fluorometric Assay Kit (BioVision, Inc., Milpitas, CA)
according to the manufacturer’s instructions, respectively.