Quantification of intracellular NADH/NAD+, NADPH/NADP+ and ATP
Strains were cultivated in YPD medium for 24 h or 72 h, and cells were harvested by centrifugation at 4°C for 30 min at 10,000×g and re-suspended in cold PBS buffer to OD600=10. The intracellular NADH/NAD and NADPH/NADP were extracted according to the previously described by Faijes et al. 23, and the intracellular ATP were extracted according to Ni et al.’s reports.24 Their concentration were measured using NADH/NAD+ Quantification Colorimeteric Kit, NADPH/NADP+ Quantification Colorimeteric Kit and ATP Colorimetric/Fluorometric Assay Kit (BioVision, Inc., Milpitas, CA) according to the manufacturer’s instructions, respectively.