PGAPZA |
Zeocinr, Expression vector with GAP promoter |
Invitrogen |
Ppic3.5k |
Kana r, Expression vector |
Invitrogen |
PGAP-Z
PPISI-Z
PGPM1-Z
PMET3-Z
PPGK1-Z
|
Zeocinr, PGAPZA plasmid with mutated LoxP site and
his4 gene
Replacing the GAP promoter with the PISI promoter in the PGAP-Z plasmid
Replacing the GAP promoter with the GPM1 promoter in the PGAP-Z plasmid
Replacing the GAP promoter with the MET3 promoter in the PGAP-Z plasmid
Replacing the GAP promoter with the PGK1 promoter in the PGAP-Z
plasmid
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This study
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PGAP-1
PGAP-2
PGAP-3
PGAP-4
PGAP-5
PPISI-1
PGPM1-1
PMET3-1
PPGK1-1
APRT-1
Pcas9-PG1-sg
Pcas9-GPD1-sg
|
PGAP-Z carrying gene zwf1
PGAP-Z carrying gene sol3
PGAP-Z carrying gene gnd2
PGAP-Z carrying gene rpe1
PGAP-Z carrying gene cPOS5
PPISI-Z carrying gene cPOS5
PGPM1-Z carrying gene cPOS5
PMET3-Z carrying gene cPOS5
PPGK1-Z carrying gene cPOS5
PGAP-Z carrying gene aprt
Ppic3.5k plasmid with PTHX1 promoter, cas9 and
PG1-sgRNA. And the his4 gene was replaced with the ARS gene of
P. pastoris
Ppic3.5k plasmid with PTHX1 promoter, cas9 and
GPD1-sgRNA. And the his4 gene was replaced with the ARS gene of
P. pastoris
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This study
This study
This study
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This study
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