YNNY
YNNY +T4
RNNR
RNNR +T4
NNNN
NNNN +T4
T4DNA Ligase 0 1 0 1 0 1
10X CutSmart™ Buffer 5 5 5 5 5 5
ATP(10μM) 5 5 5 5 5 5
adapter 200ng 200ng 200ng 200ng 200ng 200ng
Total volume 50 50 50 50 50 50
Reacted at room temperature for 1h,and T4 ligase was inactivated at 65 °C for 20 min Reacted at room temperature for 1h,and T4 ligase was inactivated at 65 °C for 20 min Reacted at room temperature for 1h,and T4 ligase was inactivated at 65 °C for 20 min Reacted at room temperature for 1h,and T4 ligase was inactivated at 65 °C for 20 min Reacted at room temperature for 1h,and T4 ligase was inactivated at 65 °C for 20 min Reacted at room temperature for 1h,and T4 ligase was inactivated at 65 °C for 20 min Reacted at room temperature for 1h,and T4 ligase was inactivated at 65 °C for 20 min