3.2 TAat adapters’ self-ligation decreased by enzyme AseI
Fig. 4 showed the effects of restriction enzyme AseI in decreasing the self-ligation of TAat adapters. TAat adapters were largely self-ligated with T4 ligase, which was virtually eliminated with the addition of restriction enzyme AseI. Different form the ClaI in prevention of adapter’s ligation, the enzyme of AseI efficiently decreased adapters’ ligation and increased the products of insert-adapter ligation (Fig. 5 lane 5). Sanger sequencing confirmed the ligation products of insert and adapter were as expected.