3.2 TAat adapters’ self-ligation decreased by enzyme AseI
Fig. 4 showed the effects of restriction enzyme AseI in decreasing the
self-ligation of TAat adapters. TAat adapters were largely self-ligated
with T4 ligase, which was virtually
eliminated with the addition of restriction enzyme AseI. Different form
the ClaI in prevention of adapter’s ligation, the enzyme of AseI
efficiently decreased adapters’ ligation and increased the products of
insert-adapter ligation (Fig. 5 lane 5). Sanger sequencing confirmed the
ligation products of insert and adapter were as expected.