2.1. Clinical samples
Samples (including whole blood, skin scrapings, and nasal plaques) were
received at Pox Virus Laboratory, ICAR-IVRI Mukteswar from Bankura, West
Bengal, India collected from a one-month-old calf suffering from a high
rise in temperature, numerous nodules all over the body, enlarged lymph
nodes along with mucopurulent nasal discharge and respiratory distress.
The scab samples were processed to make 10% suspension in PBS along
with antibiotics followed by freeze-thawing, filtration, and storage at
-80oC for further use.