3.1. Preliminary confirmation and virus isolation
DNA isolated from suspected samples yielded 192 bp PCR product in
CaPV-specific PCR (Data not shown). After infection into Vero cells,
cells were observed for 6 days for the appearance of CPE. After the
4th passage, CPE characterized by cell clustering was
observed at 3 days post-infection followed by rounding and degeneration
at the 6th day. After 6 days, cells were freeze-thawed
three times and used for further infection. Passage-5 (P5) was used for
DNA extraction.