Figures
Fig. 1. Distribution of polymorphic sites in LSDV genomes. The
sequence of LSDV-NI-2490 strain was used as the reference. Green, blue,
and red bars depict nucleotide insertions, substitutions, and deletions,
respectively. LSDV-WB/IND/19 shows very high similarity with Kenyan LSDV
strains with 10-12 variants.
Fig. 2. Pattern of LSD_019 proteins in different LSDV strains
along with predicted domains. Amino acid (aa) differences are depicted
below the protein along with the respective position at the top.
Fig. 3. Pattern of LSD_144 proteins in different LSDV strains
along with predicted domains. Amino acid (aa) differences are depicted
below the protein along with the respective position at the top.
Fig. 4. (A) Sanger sequencing across the mutation (yellow
highlighted box) detected in LSD_019 gene of LSDV-WB/IND/19 (P5, Vero
as well as original scab) and scab of LSDV-UK/IND/21 (B) alignment of
the corresponding DNA sequences with that of other LSDV strains.
Fig. 5. (A) Sanger sequencing across the mutation (yellow
highlighted box) detected in LSD_144 gene of LSDV-WB/IND/19 (P5, Vero
as well as original scab) and scab of LSDV-UK/IND/21 (B) alignment of
the corresponding DNA sequences with that of other LSDV strains.
Fig. 6. Phylogenetic analysis of LSDVs based on complete
genomes constructed using the Maximum Likelihood method using RaXML with
a bootstrap setting of 100. LSDV-WB/IND/19 strain is depicted in a
yellow box with an asterisk (*) clustering with Kenyan LSDV strains
(green box). Among LSDV, two monophyletic clusters viz. cluster 1.1
having vaccine/vaccine-associated LSDV strains and 1.2 having wild-type
LSDV strains are observed along with several novel recombinant LSDV
strains (R1, R2, R3, and R4).