Figures
Fig. 1. Distribution of polymorphic sites in LSDV genomes. The sequence of LSDV-NI-2490 strain was used as the reference. Green, blue, and red bars depict nucleotide insertions, substitutions, and deletions, respectively. LSDV-WB/IND/19 shows very high similarity with Kenyan LSDV strains with 10-12 variants.
Fig. 2. Pattern of LSD_019 proteins in different LSDV strains along with predicted domains. Amino acid (aa) differences are depicted below the protein along with the respective position at the top.
Fig. 3. Pattern of LSD_144 proteins in different LSDV strains along with predicted domains. Amino acid (aa) differences are depicted below the protein along with the respective position at the top.
Fig. 4. (A) Sanger sequencing across the mutation (yellow highlighted box) detected in LSD_019 gene of LSDV-WB/IND/19 (P5, Vero as well as original scab) and scab of LSDV-UK/IND/21 (B) alignment of the corresponding DNA sequences with that of other LSDV strains.
Fig. 5. (A) Sanger sequencing across the mutation (yellow highlighted box) detected in LSD_144 gene of LSDV-WB/IND/19 (P5, Vero as well as original scab) and scab of LSDV-UK/IND/21 (B) alignment of the corresponding DNA sequences with that of other LSDV strains.
Fig. 6. Phylogenetic analysis of LSDVs based on complete genomes constructed using the Maximum Likelihood method using RaXML with a bootstrap setting of 100. LSDV-WB/IND/19 strain is depicted in a yellow box with an asterisk (*) clustering with Kenyan LSDV strains (green box). Among LSDV, two monophyletic clusters viz. cluster 1.1 having vaccine/vaccine-associated LSDV strains and 1.2 having wild-type LSDV strains are observed along with several novel recombinant LSDV strains (R1, R2, R3, and R4).