2.4. Genome analysis, alignment, and phylogeny
The complete genome sequence of LSDV-WB/IND/19 was aligned with available genome sequences of other LSDVs from different countries as well as GTPV and SPPV sequences retrieved from GenBank included in Table 1 using MAFFT (Katoh et al., 2019). A phylogenetic tree was made with RaxML using the Maximum Likelihood construction method based on the GTR G+I with 100 bootstrap iterations (Stamatakis, 2014) and visualized by iTOL (Letunic and Bork, 2021). The nucleotide identities between CaPV genomes were calculated using the MegAlign program of DNASTAR as per the Tamura-Nei method (Tamura and Nei, 1993). The pairwise comparison between LSDV genomes was calculated using the Diffseq program (https://www.bioinformatics.nl/cgi-bin/emboss/diffseq) whereas Base‐by‐Base was used to visualize and depict the indels/SNPs (Tu et al., 2018). Prediction of protein domains was done by InterPro (Blum et al., 2020).