2.3.2. Genome Annotation
Annotation was done using a combination of annotation transfer using LSDV-NI2490 when similarity was >70% and ORF finding using homology searches on the NCBI ‘Basic Local Alignments Search Tool’ (BLAST) server with ORF >120 nucleotides and ATG as a start codon. ORFs of less than forty amino acids in length were omitted unless previously characterized in CaPVs. A total of 156 ORFs were annotated as putative genes and numbered from left to right as described previously (Tulman et al., 2001; Biswas et al., 2020). Protein identity analysis was performed using BLAST at the NCBI server. The annotated sequence of LSDV-WB/IND/19 was submitted to GenBank (Accession No. OP297402). Confirmation of nt changes in LSD_019 and LSD_144 genes was confirmed by Sanger sequencing in the same sample (Vero, P5 isolate), as well as in the original scab as well as in another scab sample, LSDV-UK/IND/21 from India.