2.3.2. Genome Annotation
Annotation was done using a combination of annotation transfer using
LSDV-NI2490 when similarity was >70% and ORF finding using
homology searches on the NCBI ‘Basic Local Alignments Search Tool’
(BLAST) server with ORF >120 nucleotides and ATG as a start
codon. ORFs of less than forty amino acids in length were omitted unless
previously characterized in CaPVs. A total of 156 ORFs were annotated as
putative genes and numbered from left to right as described previously
(Tulman et al., 2001; Biswas et al., 2020). Protein identity analysis
was performed using BLAST at the NCBI server. The annotated sequence of
LSDV-WB/IND/19 was submitted to GenBank (Accession No. OP297402).
Confirmation of nt changes in LSD_019 and LSD_144 genes was confirmed
by Sanger sequencing in the same sample (Vero, P5 isolate), as well as
in the original scab as well as in another scab sample, LSDV-UK/IND/21
from India.