2.1. Clinical samples
Samples (including whole blood, skin scrapings, and nasal plaques) were received at Pox Virus Laboratory, ICAR-IVRI Mukteswar from Bankura, West Bengal, India collected from a one-month-old calf suffering from a high rise in temperature, numerous nodules all over the body, enlarged lymph nodes along with mucopurulent nasal discharge and respiratory distress. The scab samples were processed to make 10% suspension in PBS along with antibiotics followed by freeze-thawing, filtration, and storage at -80oC for further use.