2.2 Animal model
Wild-type male C57BL/6 and FVB/N mice (8-12 weeks old) were obtained from Charles River Laboratories. All experimental protocols and animal care procedures were conducted in accordance with the Canadian Council on Animal Care and were approved by the Animal Care Committee of Western University. All mice were housed in standard caging with a 12-hour light cycle and were given standard rodent chow and water ad libitum . Eight mice from both the FVB/N and C57BL/6 strains were used as baseline controls. Both C57BL/6 and FVB/N mice were randomly divided into “control” and “cisplatin” groups. Cisplatin groups were given a single intraperitoneal injection of cisplatin (15 mg kg-1) and control mice were given an equal volume of 0.9% saline. Mice were euthanized 1, 2, 3, and 4 days after treatment for sample collection. A ketamine/xylazine mixture (100 mg kg-1and 12.5 mg kg-1, respectively) was used for anesthesia, followed by intracardiac blood collection and bilateral thoracotomy for confirmation of euthanasia. Urine samples and both kidneys were also collected upon euthanasia. Samples were also isolated from untreated C57BL/6 and FVB/N mice to be used as baseline controls. The number of samples collected varied based on sample collection/availability and exact numbers of samples collected for each sample type in each experimental group can be found in Table S1 .