2.2 Animal model
Wild-type male C57BL/6 and FVB/N mice (8-12 weeks old) were obtained
from Charles River Laboratories. All experimental protocols and animal
care procedures were conducted in accordance with the Canadian Council
on Animal Care and were approved by the Animal Care Committee of Western
University. All mice were housed in standard caging with a 12-hour light
cycle and were given standard rodent chow and water ad libitum .
Eight mice from both the FVB/N and C57BL/6 strains were used as baseline
controls. Both C57BL/6 and FVB/N mice were randomly divided into
“control” and “cisplatin” groups. Cisplatin groups were given a
single intraperitoneal injection of cisplatin (15
mg kg-1) and
control mice were given an equal volume of 0.9% saline. Mice were
euthanized 1, 2, 3, and 4 days after treatment for sample collection. A
ketamine/xylazine mixture (100 mg kg-1and 12.5 mg
kg-1, respectively) was used for anesthesia, followed
by intracardiac blood collection and bilateral thoracotomy for
confirmation of euthanasia. Urine samples and both kidneys were also
collected upon euthanasia. Samples were also isolated from untreated
C57BL/6 and FVB/N mice to be used as baseline controls. The number of
samples collected varied based on sample collection/availability and
exact numbers of samples collected for each sample type in each
experimental group can be found in Table S1 .