FIGURE LEGENDS
Figure 1 . Principle component analysis (A-C) and orthogonal parti al least squares discriminant analysis (D-F) scores plots comparing urine samples from no AKI (black) and AKI (red) patients at each timepoint: (A, D) pre, (B, E) 24-48h, and (C, F) post. Model statistics for OPLS-DA were as follows: (D) pre: R2Y = 0.784, Q2Y = 0.389 (E) 24-48h: R2Y = 0.752, Q2Y = 0.384 (F) post: R2Y = 0.969, Q2Y = 0.700. n=18-20 for no AKI patients and n=10-11 for AKI patients.
Figure 2. Principle component analysis (A-C) and orthogonal partial least squares discriminant analysis (D-F) scores plots comparing serum samples from no AKI (black) and AKI (red) patients at each timepoint: (A, D) pre, (B, E) 24-48h, and (C, F) post. Model statistics for OPLS-DA were as follows: (D) pre: R2Y = 0.991, Q2Y = 0.668 (E) 24-48h: R2Y = 0.776, Q2Y = 0.317 (F) post: R2Y = 0.995, Q2Y = 0.813. n=18-20 for no AKI patients and n=10-11 for AKI patients.
Figure 3 . (A-D ) Log transformed relative intensity of urine features significantly different between no AKI and AKI patients at the pre timepoint. Data are presented as box and whisker plots, where boxes represent the median and interquartile range (IQR), and whiskers represent 1.5x IQR. (F) Diagnostic performance of urine metabolites as assessed by the area under the receiver operating curve. Statistical analysis was performed using two-way ANOVA, *p<0.05, **p<0.01 compared to no AKI patients at each timepoint. n=18-20 for no AKI patients and n=10-11 for AKI patients.
Figure 4. (A-J ) Log transformed relative intensity of urine features significantly different between no AKI and AKI patients at the post timepoint. Data are presented as box and whisker plots, where boxes represent the median and interquartile range (IQR), and whiskers represent 1.5x IQR. (K) Diagnostic performance of urine metabolites as assessed by the area under the receiver operating curve. Statistical analysis was performed using two-way ANOVA, *p<0.05, **p<0.01, ***p<0.001 compared to no AKI patients at each timepoint. n=18-20 for no AKI patients and n=10-11 for AKI patients.
Figure 5. (A-G ) Log transformed relative intensity of serum features significantly different between no AKI and AKI patients at the post timepoint. Data are presented as box and whisker plots, where boxes represent the median and interquartile range (IQR), and whiskers represent 1.5x IQR. (H) Diagnostic performance of urine metabolites as assessed by the area under the receiver operating curve. Statistical analysis was performed using two-way ANOVA, *p<0.05, **p<0.01, ***p<0.001 compared to no AKI patients at each timepoint. n=18-20 for no AKI patients and n=10-11 for AKI patients.
Figure 6. Log transformed relative intensity of urine and serum features significantly altered over time in no AKI patients. Data are presented as box and whisker plots, where boxes represent the median and interquartile range (IQR), and whiskers represent 1.5x IQR. Statistical analysis was performed using one-way ANOVA, *p<0.05, **p<0.01, ***p<0.001 compared to saline control at each timepoint, n=18-20.