Abstract
Yarrowia lipolytica , an oleaginous yeast with the GRAS status,
has been developed as a platform for the chemical production.
Specifically, promoter engineering is an important approach to
regulating gene expression at the transcriptional level, which is of
great significance in constructing microbial cell factories. Until now,
some work on the promoter study has been carried out in Y.
lipolytica . However, compared with other microorganisms, such asSaccharomyces cerevisiae , Bacillus subtilis , andetc. , obtained achievements still need further replenishment and
development for Y. lipolytica . For this, we screened 81
endogenous promoters in Y. lipolytica , mainly involved in carbon
metabolism, amino acid metabolism, and lipid metabolism. As a result, we
obtained 15 strong promoters, 41 medium strength promoters, and 25 weak
promoters, with the strength spanning from 0.06% to 1.60-fold of
PTEF promoter. In general, our study provides a unique
and available endogenous promoter library for Y. lipolytica .
Keywords: Promoters, Yarrowia lipolytica , Promoter
library, Synthetic biology, Microbial cell factories