Figure
1 - The for gene in V. canescens. A) Maximum
likelihood phylogenetic tree of the for gene in insects.
Sequences used from 39 species of insects: hemiptera (2), orthoptera
(2), coleoptera (4), lepidoptera (6), diptera (15), hymenoptera (10).
Sequence from V. canescens figured in bold within hymenoptera
cluster. One crustacean (Daphnia pulex ) and one mammal (Mus
musculus ) sequences were used as outgroups. Phylogeny was constructed
from the 533 aminoacids located in the conserved C-terminal region of
the protein. Branch support was estimated by likelihood-ration test,
aLRT values ≥0.90 were indicated with empty circles, and ≥0.95 with full
circles. B) Schematic of the for gene model and 17 associated
isoforms identified in V. canescens. The for gene located
on the scaffold 64 of V. canescens genome was constituted of 13
exons (dark blue boxes). The two conserved functional protein domains
(two cGMP binding domains and protein kinase domain) were located at the
3’ extremity of the gene sequence and were represented under the genome
sequence (in blue and red respectively). The 5’ part of the gene
exhibited variations, with 4 distinct promotors identified
(pr1-4 , dark blue arrows), and the corresponding transcripts were
labelled accordingly (Vcan27706 to Vcan27709 ). A total of
17 isoforms were identified within RNA-seq libraries issued from sexual
and asexual V. canescens heads, and were all represented under
the genome sequence with coding sequences figured in dark grey boxes and
non-coding sequences (5’ and 3’ UTRs) in pale grey boxes. The amplicon
used to quantify for transcript with RT-qPCR was common to almost
all isoforms (excepted Vcan_27707i12 ) and figured in green.