Reagents
Opioid ligands U50,488H, naloxone and nor-BNI (nor-binaltorphimine) were purchased from Tocris Bioscience (Cookson MI, USA). Dynorphin1-13, pertussis toxin, bafilomycin A1, phosphatase inhibitors and TRI-reagent for RNA extraction were from Sigma Aldrich (St Louis, MO, USA). Protease inhibitors were from Roche (Roche Diagnostics, Basel, Switzerland). Protein A/G agarose beads and PD98059 (MEKI inhibitor SC-3532) were from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Kapa Hi-Fi PCR, Kapa SYBR Fast QPCR kits for ChIP assay and Real-time PCR respectively were purchased from Kapa Biosystems (Roche, IN, USA). All reagents were purchased from Sigma Aldrich (Sigma Aldrich MI, USA)