Statistical analysis
Statistical analysis was performed using one or two-way analysis of
variance (ANOVA) following by Tukey’s t test for post-hoc
comparisons. All experiments were repeated at least three times. Bands
were quantified by densitometric analysis using Image J software
(National Institute of Health, Bethesda, MD, USA) and expressed as mean
± SEM. Representative experiments are shown and statistical significance
is shown in each figure legend.