Reagents
Opioid ligands U50,488H, naloxone and nor-BNI (nor-binaltorphimine) were
purchased from Tocris Bioscience (Cookson MI, USA).
Dynorphin1-13, pertussis toxin, bafilomycin A1,
phosphatase inhibitors and TRI-reagent for RNA extraction were from
Sigma Aldrich (St Louis, MO, USA). Protease inhibitors were from Roche
(Roche Diagnostics, Basel, Switzerland). Protein A/G agarose beads and
PD98059 (MEKI inhibitor SC-3532) were from Santa Cruz Biotechnology
(Santa Cruz, CA, USA). Kapa Hi-Fi PCR, Kapa SYBR Fast QPCR kits for ChIP
assay and Real-time PCR respectively were purchased from Kapa Biosystems
(Roche, IN, USA). All reagents were purchased from Sigma Aldrich (Sigma
Aldrich MI, USA)