2.5.2 Determination of components in medium
Glucose consumption and bacterial metabolite production were analyzed using HPLC (LC-20AD, Shimadzu, Kyoto, Japan) with an Aminex HPX-87H column (Bio-Rad, Hercules, CA, USA). The mobile phase was 5 mM H2SO4 and the flow rate was 0.6 mL/min at 65 ℃.