3.9 Long-term culture
Because of their large surface area relative to volume, nanofibrous
substrates might offer more binding sites for initial cell attachment,
in addition to longer periods of cell culture. However, few studies have
investigated long-term maintenance of C2C12 cells cultivated onto
scaffolds. In order to observe how C2C12 cells colonize both types of
scaffolds, C2C12 was seeded onto the nanofibers and cultivated for 14,
21, and 28 days in GM for subsequent SEM analysis (Figure 13).