3.9 Long-term culture
Because of their large surface area relative to volume, nanofibrous substrates might offer more binding sites for initial cell attachment, in addition to longer periods of cell culture. However, few studies have investigated long-term maintenance of C2C12 cells cultivated onto scaffolds. In order to observe how C2C12 cells colonize both types of scaffolds, C2C12 was seeded onto the nanofibers and cultivated for 14, 21, and 28 days in GM for subsequent SEM analysis (Figure 13).