Fig 7A. C2C12 cell attachment to cellulose acetate (CA) and cellulose acetate nanofibers with annatto extract (CA@A) determined by cell counting after 24 h.
The results showed that fewer than 1,500 cells/well were unable to adhere to any substrate. The rates of adherent cells were approximately 97.5% and 98% for cells cultivated onto CA and CA@A scaffolds, respectively. No statistically significant difference was found between the cell number in the CA and CA@A nanofiber scaffolds, suggesting that both scaffolds permit significant cellular attachment.
The MTT assay was used to evaluate the viability and proliferation of C2C12 cells on CA and CA@A nanofibers.