Figure 8. SEM images at different magnifications of C2C12 cells cultivated onto cellulose acetate nanofiber (A, B, C) and cellulose acetate nanofiber with annatto extract (D, E, F) after two days. Scale bars indicate (A,D) 200 µm; (B, E) 50 µm; (C, F) 5 µm.
Figure 8 shows the morphology of C2C12 cells in nanofibers after 2 days of cell culture. The myoblasts can be seen in groups in both CA and CA@A nanofibers; more cell groups can be seen in the CA@A nanofiber (Figure 8 D). The magnified image reveals these interactions between neighboring cells, probably establishing the first cell-cell contacts (Figure 8 B, E). The cells were also well linked to the nanofibers (Figure 8 C, F) and had spindle-shaped morphology, similar to mononucleated myoblasts [45].