2.5 Cell culture
Immortalized mouse myoblasts (C2C12) were seeded onto the nanofibers (CA and CA@A) and onto the monolayer as a control. Before cell seeding, scaffolds were sterilized using gamma irradiation. The materials were irradiated at room temperature with a standard dose of 10 kGy.60Co gamma-ray source was used. Gamma irradiation sterilization was carried out at Gamma Irradiation Laboratory installed at the Nuclear Technology Development Centre (CDTN), Belo Horizonte, Brazil. Both scaffolds were equilibrated using 200 μl of growth medium (GM) [GM: DMEM-high glucose (Gibco), supplemented with 10% bovine fetal serum (Gibco) and 1% anti-anti (Gibco)] for 24 h before cell seeding. C2C12 cells were used in the 4th and 8th passages, in triplicate. Cells were seeded at a density of 8x104 cells/well in 24-well plates. After 2 h of incubation at 37°C and 5% CO2, the volume of GM was completed to 500 μl/well.