Figure 8. SEM images at different magnifications of C2C12 cells
cultivated onto cellulose acetate nanofiber (A, B, C) and cellulose
acetate nanofiber with annatto extract (D, E, F) after two days. Scale
bars indicate (A,D) 200 µm; (B, E) 50 µm; (C, F) 5 µm.
Figure 8 shows the morphology of C2C12 cells in nanofibers after 2 days
of cell culture. The myoblasts can be seen in groups in both CA and CA@A
nanofibers; more cell groups can be seen in the CA@A nanofiber (Figure 8
D). The magnified image reveals these interactions between neighboring
cells, probably establishing the first cell-cell contacts (Figure 8 B,
E). The cells were also well linked to the nanofibers (Figure 8 C, F)
and had spindle-shaped morphology, similar to mononucleated myoblasts
[45].