2.6 MTT assay for cell viability
Cell viability was assessed using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) (Invitrogen), according to the manufacturer’s instructions.
C2C12 cells were seeded onto the scaffolds or as a monolayer at a density of 8x104 cells/well in a 24-well plate in GM. After 2 and 7 days, GM was replaced with the MTT solution, and the samples were incubated for 2 h at 37 °C and 5% CO2. Formazan crystals were then dissolved in 1 ml/well of isopropanol-acid (100 ml isopropanol:134 ul of hydrochloric acid). Next, the solution was then transferred to a 96-well plate in triplicate and absorbances were measured at 595 nm using a microplate reader (ELX800 device; BioTek, Winooski, USA).