Fig.4
3.4 Optimal temperature
The optimal reaction temperature
of free D-amino acid dehydrogenase with peptide linker was investigated.
As it can be seen from Fig.5, the optimal temperature was 40 ℃. Relative
activity could keep above 80% at 40-80 ℃, the relative activity was
97.7% at 80 ℃. The results showed that the DAADH can maintain high
catalytic activity under high temperature. In high temperature such as
70-80 ℃, peptide linker cause conformation adjustment of enzyme, which
may obtain high activity.