Fig.S2

3.2 Effect of ionic liquids

Ionic liquids (ILs) are attractive in biocatalysis as ILs’ unique chemical properties. Several ionic liquids ([EMIM]BF4, [BMIM]Cl, [BMIM]BF4) were selected and added into the reaction system with final concentration as 5 mM. [EMIM]BF4and [BMIM]Cl reduced the relative enzyme activity (DAADH) to 47.7% and 68.6% respectively, while the activity of DAADH in [BMIM]BF4 ionic liquid was 97.8% of the initial enzyme activity (Fig.3).
The electrostatic interactions between Ionic liquid and amino acid residues could affect enzyme activity [22]. Solvation effects is also a factor to be considered. [BMIM]BF4 displays an excellent miscibility with water which could be beneficial to enzyme activity [23].