Table 1
3.9 Morphology analysis
The morphology of the immobilized-DAADH was studied. SEM image indicated
a mesoporous structure with pore sizes of different scales (Fig.9). This
mesoporous structure probably facilitates the substrate’s diffusion.
ZIF-8 provides a biocompatible carrier for DAADH immobilization. The
interaction of ZIF-8 and the peptide linker improved the stability of
the immobilized enzyme. With the addition of RGO, the immobilized enzyme
DAADH/ZIF-8/RGO showed amorphological layers.
The addition of RGO can form hybrid material (ZIF-8/RGO) with higher
biocompatibility, which may due to hydrophobic interaction between RGO
and DAADH [24]. ZIF-8/RGO composites can effectively protect the
active conformation of enzymes and improve the stability of enzymes in
extreme conditions such as high temperature and high pH value. The
DAADH/ZIF-8/RGO is expected to be further applied in biocatalysis
[29].