Fig.6

3.6 Reuse stability

The reuse stability of immobilized enzymes was investigated, as shown in Fig.7. After 7 consecutive cycles of reactions, the residual activities of DAADH/ZIF-8/RGO and DAADH/ZIF-8 were 64.3% and 45.6%, respectively. Enhanced stability of DAADH/ZIF-8/RGO probably as DAADH tends to combine with the hydrophobic surface of RGO. Hydrophobic effect greatly improves enzyme recovery activity and shows better reuse stability than immobilization by DAADH/ZIF-8.