3. Results and discussion
3.1 Effects of metal ions
Several divalent metal ions (Ca2+,
Mn2+, Zn2+, Mg2+,
Ni2+, Cu2+ and
Ba2+) were selected to investigate the effect of metal
ions on DAADH activity (Fig.2a). Ni2+ activated DAADH
by 128.3%. Ca2+, Ba2+,
Mg2+ inhibit the activity of DAADH. The effect of
Ni2+ concentrations on enzyme activity was studied as
shown (Fig.2b). Maximum enzyme activity was obtained with 15 mM
Ni2+, which was 161.2% of the initial activity .