Fig.S2
3.2 Effect of ionic liquids
Ionic liquids (ILs) are
attractive in biocatalysis as ILs’ unique chemical properties. Several
ionic liquids ([EMIM]BF4, [BMIM]Cl,
[BMIM]BF4) were selected and added into the reaction
system with final concentration as 5 mM. [EMIM]BF4and [BMIM]Cl reduced the relative enzyme activity (DAADH) to 47.7%
and 68.6% respectively, while the activity of DAADH in
[BMIM]BF4 ionic liquid was 97.8% of the initial
enzyme activity (Fig.3).
The electrostatic interactions between Ionic liquid and amino acid
residues could affect enzyme activity [22]. Solvation effects is
also a factor to be considered. [BMIM]BF4 displays
an excellent miscibility with water which could be beneficial to enzyme
activity [23].