Concluding remarks
In conclusion, recombinant FPOX is prone to form a high level of
inclusion body. FPOX inclusion bodies are heterogenous in size and can
be decreased using signal peptide. Freeze-thawing method can be used to
solubilize FPOX inclusion bodies at low concentrations of urea.
Compounds such as arginine and sorbitol at concentrations of 0.2 and 0.3
M, respectively, showed no considerable effect on protein solubility.
FPOX can be secreted into periplasmic space as active form.