2.5 Arginine and sorbitol stabilizers and their effects on solubility of rFPOX
Arginine and sorbitol with concentrations of 0.2 and 0.3 M, respectively, were added to the LB medium and the production of recombinant FPOX was performed in the presence of these compounds. The induction of protein expression was done by adding 0.1 mM IPTG and incubation at temperature of 16 °C for overnight.