2.7 The investigation of the effect of different proteases on hemoglobin as substrate
For this purpose, different proteases including Neutral protese, Alkaline protease, Pepsin, Papain and Chymotrypsin (1:100 W/W of enzyme to substrate) were applied to digest hemoglobin in order to produce substrate (f-ValHis) for FPOX enzyme. The digestion of hemoglobin by the proteases was evaluated by SDS-page analysis.