3.1 Expression of FPOX
Recombinant FPOX, approximately 47 KDa, was mainly expressed as
inclusion bodies in BL21 and shuffle strains under different conditions
of IPTG and induction temperature (Fig.1) and no considerable soluble
protein was observed in supernatant. The level of protein expression was
same in both of strains. The different physicochemical characteristics
were evaluated by protparam tool, and shown that, FPOX composed of 437
amino acids with a molecular weight of about 48 KDa and contained a
theoretical pI of 6.68. This molecule consisted of 229 hydrophobic amino
acids (52.4 %) and 208 hydrophilic residues (47.6 %) and, the acidic
and basic amino acids were 51 (11.7 %) and 62 (14.2 %), respectively.