3.4 The effect of arginine and sorbitol in culture medium on the
formation of inclusion body
The effect of arginine and sorbitol on protein solubility was
investigated in shuffle strain. Results show that nearly a similar
amount of protein expression was observed in the presence of sorbitol
compared to control (Fig.4). In the other side, in the presence of
arginine in the culture media the expression of rFPOX was very low and
concluded that arginine had the least effect on solubility compared to
sorbitol. The concentration of total protein in the presence of sorbitol
(2.43 mg/ml) was higher than arginine and control culture media (2.13
mg/ml).