2.13 | Statistical Analyses
The data and statistical analysis comply with the recommendations of theBritish Journal of Pharmacology on experimental design and
analysis in pharmacology (Curtis et al., 2022). All tests were treated
as independent values rather than technical replicates. Data are
expressed as the means ± SEM and represent at least five independent
experiments. Two-group differences were analyzed using independent
sample t test. One-way ANOVA followed by Tukey’s post hoc test was used
to compare more than two groups of data. Post hoc tests were conducted
only if F in ANOVA (or equivalent) achieved the ‘chosen’ necessary level
of statistical significance and there was no significant variance
inhomogeneity. Statistical significance was set at P < 0.05.
All analyses were performed using the GraphPad Prism 8 (GraphPad; San
Diego, CA, USA).