2.13 | Statistical Analyses
The data and statistical analysis comply with the recommendations of theBritish Journal of Pharmacology on experimental design and analysis in pharmacology (Curtis et al., 2022). All tests were treated as independent values rather than technical replicates. Data are expressed as the means ± SEM and represent at least five independent experiments. Two-group differences were analyzed using independent sample t test. One-way ANOVA followed by Tukey’s post hoc test was used to compare more than two groups of data. Post hoc tests were conducted only if F in ANOVA (or equivalent) achieved the ‘chosen’ necessary level of statistical significance and there was no significant variance inhomogeneity. Statistical significance was set at P < 0.05. All analyses were performed using the GraphPad Prism 8 (GraphPad; San Diego, CA, USA).