2.5 | Cell culture
Mouse renal tubular epithelial cells (mTECs) were kindly provided by Huiyao Lan. TECs were cultured in DMEM/F-12 (HyClone, Logan, UT, USA) supplemented with 5% (v/v) heat-inactivated fetal bovine serum (Merck Millipore, Darmstadt, Germany) at 37 °C in a humidified incubator under 5% CO2. After overnight starvation in DME/F12 medium with 0.5% FBS, mTECs were treated with AAI (10 μM) for 24 h.