FIGURE 5. Proline-serine-threonine-phosphatase-interacting protein 2 (PSTPIP2) overexpression suppressed AAI-induced kidney injury and PANoptosis.
(A) Schematic illustrating the genetic approach used for generating PSTPIP2-KI mice. (B) The schematic diagram depicts the animal experimental design. PSTPIP2-KI mice aged 6–8 weeks were treated with AAI and were euthanized three days after AAI treatment. (C) PSTPIP2 mRNA was assessed using PCR in PSTPIP2-KI mice compared with WT. (D) Creatinine (Cr) and blood urea nitrogen (BUN) were assessed in WT, AAI-treated WT mice, untreated PSTPIP2-KI, and AAI-treated PSTPIP2-KI mice three days after AAI treatment. (E) Hematoxylin-eosin (HE)-stained kidney tissue section (Scale bar = 40 μm). (F) Photomicrographs of immunohistochemical of cleaved-caspase-3, pMLKL, and GSDMD-N in WT, AAI-treated WT mice, untreated PSTPIP2-KI, and AAI-treated PSTPIP2-KI mice (Scale bar = 40 μm). Data are presented as the mean ± SEM, n = 5. ns, not significant; *P < 0.05, significantly different, as indicated.