FIGURE 7. AAV9-mediated PSTPIP2 restoration re-induces renal injury and PANoptosis in PSTPIP2-KI mice.
(A) Schematic illustrating the rescue experiment in PSTPIP2-KI mice. (B) Imaging of LUC-labeled AAV9 in mice. (C) Creatinine (Cr) and blood urea nitrogen (BUN) were assessed. (D) Hematoxylin-eosin (H&E)-stained kidney tissue section (Scale bar = 40 μm). (E) Photomicrographs of immunohistochemical of cleaved-caspase-3, pMLKL, and GSDMD-N (Scale bar = 40 μm). Data are presented as the mean ± SEM, n = 5. ns, not significant; *P < 0.05, significantly different, as indicated.
FIGURE 8. FK-228 does not affect AAI-induced renal injury and PANopsis under PSTPIP2 silencing.
(A) Western blotting of cleaved-caspase-3, pMLKL, and GSDMD-N. (B) Western blotting of cleaved-caspase-3, pMLKL, and GSDMD-N in mTECs treated with siRNA-PSTPIP2 and FK-228. (C) Immunofluorescence staining of cleaved-caspase-3, pMLKL, and GSDMD-N in mTECs treated with siRNA-PSTPIP2 and FK-228. (Scale bar = 100 μm). Data are presented as the mean ± SEM, n = 5. ns, not significant; *P < 0.05, significantly different, as indicated.