FIGURE 2. Effects of histone deacetylase (HDAC) inhibitor on
AAI-induced nephrotoxicity and PANoptosis.
(A) Expression level of different isoforms of HDAC at 24, 48, and 72 h
after AAI administration. (B) The schematic diagram depicts the animal
experimental design. C57BL/6 mice aged 6–8 weeks were treated with AAI
and FK-228 every 24 h for two days after AAI administration. (C)
Creatinine (Cr) and blood urea nitrogen (BUN) after different
treatments. (D) Hematoxylin-eosin (H&E)-stained kidney tissue section
(Scale bar = 40 μm). (E) Photomicrographs of immunohistochemical of
cleaved-caspase-3, pMLKL, and GSDMD-N in AAI-treated AKI mice
administered with FK-228 (Scale bar = 40 μm). Data are presented as the
mean ± SEM, n = 5. ns, not significant; *P < 0.05,
significantly different, as indicated.