2.2 | AAI Administration to Mice
Six- to eight-week-old male C57BL/6 mice were randomly divided into two groups (n = 5 per group): Mice were administered AAI (10 mg/kg, i.p.) or isotonic sodium chloride solution as a single intraperitoneal injection. Mice were euthanized 72 h after AAI administration. Tissue and blood were collected for further analysis. Kidney function and injury were assessed using urine protein, serum creatinine, and histology.