2.4 | AAV9-mediated PSTPIP2 inhibition in PSTPIP2-KI mice
Adeno-associated viruses were developed and obtained from the HANBIO Company (Shanghai, China). Six- to eight-week-old male C57BL/6 mice were divided into four groups, with five animals in each group. Mice in the wild-type control group were administered isotonic sodium chloride solution in a single i.p. injection. Wild-type AAI mice were administered AAI at 10 mg/kg via a single i.p. injection. PSTPIP2-KI group mice were injected with an empty adeno-associated virus 9(AAV9) vector using tail vein injection and administered AAI at 10 mg/kg in a single i.p. injection. The PSTPIP2-KI group mice were injected with an AAV9-PSTPIP2 vector via tail vein injections. Mice were imaged after injection with 150 mg/kg d-luciferin. AAI was administered at 10 mg/kg in a single i.p. injection. Animals were euthanized 72 h after AAI injection, and blood and kidney tissues were collected.