2.8 | Chromatin Immunoprecipitation Assay
ChIP assays were performed using a SimpleChIP® enzymatic chromatin IP kit with magnetic beads (#9004; Cell Signaling Technology, USA) according to the manufacturer’s instructions. Chromatin DNA was immunoprecipitated using rabbit anti-H3K27Ac and normal rabbit IgG. The immunoprecipitated DNA was purified using a DNA purification column from the kit and analyzed by PCR using the forward primer 5′-GGAGAGGAGAGGAGAGGAGAGGAG-3′ and reverse primer 5′-ACTACGAGTACGAGTGACACTGAGG-3′ specific for the PSTPIP2 proximal promoter region.