FIGURE 4. Histone deacetylase (HDAC) inhibitor Romidepsin
(FK228, Depsipeptide) upregulates
Proline-serine-threonine-phosphatase-interacting protein 2 (PSTPIP2)
expression in kidney epithelial cells in vivo and in vitro.
(A) The PSTPIP2 protein levels in AAI-treated AKI mice administered with
FK-228 analyses using western blot. (B) PSTPIP2 mRNA was assessed using
real-time quantitative polymerase chain reaction (real-time qPCR) in
AAI-treated AKI mice administered with FK-228. (C) The PSTPIP2 protein
levels in AAI-treated mTECs treated with FK-228 analyses using western
blot. (D) PSTPIP2 mRNA was assessed using real-time qPCR in AAI-treated
mTECs treated with FK-228. (E) Chromatin immunoprecipitation assay of
acetylated histone in the PSTPIP2 promoter region. (F) The H3K27Ac
protein levels in AAI-treated AKI mice administered with FK-228 were
analyzed using a western blot. (G) The H3K27Ac protein levels in
AAI-treated mTECs treated with FK-228 analyses using western blot. Data
are presented as the mean ± SEM, n = 5. ns, not significant; *P
< 0.05, significantly different, as indicated.