FIGURE 4. Histone deacetylase (HDAC) inhibitor Romidepsin (FK228, Depsipeptide) upregulates Proline-serine-threonine-phosphatase-interacting protein 2 (PSTPIP2) expression in kidney epithelial cells in vivo and in vitro.
(A) The PSTPIP2 protein levels in AAI-treated AKI mice administered with FK-228 analyses using western blot. (B) PSTPIP2 mRNA was assessed using real-time quantitative polymerase chain reaction (real-time qPCR) in AAI-treated AKI mice administered with FK-228. (C) The PSTPIP2 protein levels in AAI-treated mTECs treated with FK-228 analyses using western blot. (D) PSTPIP2 mRNA was assessed using real-time qPCR in AAI-treated mTECs treated with FK-228. (E) Chromatin immunoprecipitation assay of acetylated histone in the PSTPIP2 promoter region. (F) The H3K27Ac protein levels in AAI-treated AKI mice administered with FK-228 were analyzed using a western blot. (G) The H3K27Ac protein levels in AAI-treated mTECs treated with FK-228 analyses using western blot. Data are presented as the mean ± SEM, n = 5. ns, not significant; *P < 0.05, significantly different, as indicated.