3.3 | HDAC inhibitor upregulated PSTPIP2 expression in vivo
PSTPIP2 plays a vital role in tumors, autoimmune diseases, and other diseases by influencing cell proliferation, apoptosis, and the secretion of inflammatory factors. In the AAN model, we observed that AAI treatment decreased the expression of PSTPIP2 in tubular epithelial cells; PSTPIP2 expression increased in the presence of FK-228 in vivo and in vitro (Figure 4A–D). Additionally, the H3K27Ac protein level was downregulated in vivo and in vitro, and FK-228 increased the protein level (Figure 4F, G). Chromatin immunoprecipitation (ChIP) assays revealed that AAI treatment reduced histone acetylation in the promoter region of PSTPIP2. Notably, the FK-228 treatment increased histone acetylation in the promoter region of the PSTPIP2 gene (Figure 4E).