FIGURE 3. Histone deacetylase (HDAC) inhibitor romidepsin
(FK228, Depsipeptide) treatment reduced the kidney’s AAI-induced tubular
epithelial cell PANoptosis.
(A) Cleaved-Caspase-3, pMLKL, and GSDMD-N protein levels in AAI-treated
AKI mice administered with FK-228 analyses using western blot. (B)
Immunofluorescence staining of cleaved-caspase-3, pMLKL, and GSDMD-N in
AAI-treated mTECs treated with FK-228 (Scale bar = 100 μm). (C) Western
blot analyses of cleaved-caspase-3, pMLKL, and GSDMD-N in AAI-treated
mTECs treated with FK-228. Data are presented as the mean ± SEM, n = 5.
ns, not significant; *P < 0.05, significantly different, as
indicated.