2.8 | Chromatin Immunoprecipitation Assay
ChIP assays were performed using a SimpleChIP® enzymatic chromatin IP
kit with magnetic beads (#9004; Cell Signaling Technology, USA)
according to the manufacturer’s instructions. Chromatin DNA was
immunoprecipitated using rabbit anti-H3K27Ac and normal rabbit IgG. The
immunoprecipitated DNA was purified using a DNA purification column from
the kit and analyzed by PCR using the forward primer
5′-GGAGAGGAGAGGAGAGGAGAGGAG-3′ and reverse primer
5′-ACTACGAGTACGAGTGACACTGAGG-3′ specific for the PSTPIP2 proximal
promoter region.