3.3 | HDAC inhibitor upregulated PSTPIP2 expression in
vivo
PSTPIP2 plays a vital role in tumors, autoimmune diseases, and other
diseases by influencing cell proliferation, apoptosis, and the secretion
of inflammatory factors. In the AAN model, we observed that AAI
treatment decreased the expression of PSTPIP2 in tubular epithelial
cells; PSTPIP2 expression increased in the presence of FK-228 in vivo
and in vitro (Figure 4A–D). Additionally, the H3K27Ac protein level was
downregulated in vivo and in vitro, and FK-228 increased the protein
level (Figure 4F, G). Chromatin immunoprecipitation (ChIP) assays
revealed that AAI treatment reduced histone acetylation in the promoter
region of PSTPIP2. Notably, the FK-228 treatment increased histone
acetylation in the promoter region of the PSTPIP2 gene (Figure 4E).