FIGURE 3. Histone deacetylase (HDAC) inhibitor romidepsin (FK228, Depsipeptide) treatment reduced the kidney’s AAI-induced tubular epithelial cell PANoptosis.
(A) Cleaved-Caspase-3, pMLKL, and GSDMD-N protein levels in AAI-treated AKI mice administered with FK-228 analyses using western blot. (B) Immunofluorescence staining of cleaved-caspase-3, pMLKL, and GSDMD-N in AAI-treated mTECs treated with FK-228 (Scale bar = 100 μm). (C) Western blot analyses of cleaved-caspase-3, pMLKL, and GSDMD-N in AAI-treated mTECs treated with FK-228. Data are presented as the mean ± SEM, n = 5. ns, not significant; *P < 0.05, significantly different, as indicated.