3.6 | PSTPIP2 regulates PANoptosis through Caspase-8
Cysteinyl aspartate specific proteinase (Caspase)-8 has long been
considered a promoter of apoptosis and part of the mechanism by which
cytotoxic drugs kill cancer cells (Jiang, Qi, Li, Wu, Song & Li, 2021).
Caspase-8 controls the interactions between apoptosis, necroptosis, and
pyroptosis and determines the type of cell death induced by the
activation of cell death signaling (Schwarzer, Laurien & Pasparakis,
2020). A previous study reported a significantly higher number of
caspase-8 in PSTPIP2 knockout mice than in the control group (Gurung,
Burton & Kanneganti, 2016). Thus, to determine whether PSTPIP2
decreases PANoptosis expression by inhibiting caspase-8, we examined the
effect of PSTPIP2 on caspase-8 expression. Notably, we observed a
significantly lower number of caspase-8 in PSTPIP2 overexpression groups
than in the WT controls (Figure 9A–D). To further elucidate the
relationship between PSTPIP2 and Caspase-8, we also verified that
Caspase-8 expression increases with the inhibition of PSTPIP2 after
PSTPIP2 was inhibited (Figure 10A–D).