Monofoil and permutant domain-swapped interfaces
Varying degree of steric entanglement was exhibited by Monofoil and circularly permuted forms. Monofoil exhibited the least steric entanglement, with a dissociation midpoint of ~1.0 J/Kg (like the antiparallel gauche+/gauche+ coiled coil). Permutant P3 exhibited an entanglement midpoint of ~4 J/Kg (like the antiparallel trans/trans coiled coil). Permutant P1 exhibited a greater entanglement midpoint of ~7 J/Kg. Permutant P2 was remarkable in having an entanglement energy that prevented dissociation even at the highest evaluated energy of 120 J/Kg) (Fig. 5).