3.2. Quantification of DNA
The DNA thus extracted was measured for its concentration by QuantusTM Fluorometer® as per the manufacturer’s protocol. For the method 1, the final concentration of genomic DNA was 4.11ng/µl, similarly, in method 2, method 3 and method 4 are 11.49ng/µl, 17.81 ng/µl and 53.98ng/µl respectively (Figure 1). The concentration of method 4 is higher than the other three methods and approximately equal to the commercial faecal DNA extraction Kits 1 and 2. These commercial kits were used as an internal quality control while extracting the DNA by current methods. The DNA yield obtained for Kit 1 and 2 is 49.76ng/µl and 56.93ng/µl respectively.
(Figure 1 here)