2.7. Method 4
In this method sonication step was not included, instead, the
’snap-freeze-thaw’ method is replaced with snap-chill (at
-80ჿC) for 10 minutes and thaw (with higher
temperature) at 95ჿC (instead of
70ჿC) for 5 minutes and this snap-chill-boil cycle
repeated two more times with vortexing for 30 seconds between each cycle
(Table 1). 10 µl of DNase-Free-RNase were spiked in the sample after
cooling in ice for 2 minutes and incubated at 37ºC for 10 minutes to
improve the quality of DNA, The remaining protocol was performed as per
method 1.
(Table 1 here)