2.5. Method 2
The major challenge for the extraction of Cryptosporidium DNA is
to lyse the thick wall of oocysts. Since pretreatment steps like
snap-freeze and thawing facilitate rupture of the oocyst wall, we have
employed the same using Liquid nitrogen (-196ჿC) and
incubation at 70ჿC respectively, instead of the
sonication step used in the previous method. The remaining
steps/procedure performed was similar to method 1.