2.3. Solution used
Extraction buffer was prepared in 2.0% cetyltrimethylammonium bromide
(CTAB), which consist 100mMTris-HCl (pH 8.0), 1.4 M NaCl, 20mM
ethylenediaminetetraaceticacid (EDTA). Absolute Isopropanol, 70%
ethanol (v/v); Phenol: Chloroform: Isoamyl alcohol (25:24:1, v/v),
Chloroform: Isoamyl alcohol (24:1, v/v), TE (Tris/EDTA) buffer
preparation: 10mM Tris-HCl (pH 8.0); and 1mM EDTA (pH 7.4).