Significance of the study
- This method proved that it is simple and highly economical for
laboratories with minimal funding, and on the other hand commercial
faecal DNA kits are very costly and offer a limited sample size for
the extraction of Cryptosporidium genomic DNA.
- Method 4 with minimal steps devoid of sonication and
LN2 steps could extract good quality DNA using the
freeze-boil-CTAB method as assessed by validation with conventional
PCR and real-time- PCR (RT-PCR).
- Most importantly this conventional protocol for DNA extraction
developed in the current study could match the spin column-based kits
in terms of successful removal of the PCR inhibitors as evidenced by
the successful replication of results by molecular tests.