Legends to Figures
Figure 1: Total DNA extracted from Cryptosporidiumfaecal sample (Lane 1- Method 1, lane 2- Method 2, lane 3- Method 3, Lane4- Method 4, Lane 5- Kit 1, Lane 6- Kit 2) using present modified method visible on 1.8% agarose gel stained with ethidium bromide. Lane M is 100bp ladder.
Figure 2 (A): Cyclic conditions for DNA duplex TaqMan® probe real time PCR (B) Nested PCR amplicon of 18SSU rRNA gene on 1.8% agarose gel with arrow indicates the 18SSU rRNA gene product (Lane 1- Method 1, lane 2- Method 2, lane 3- Method 3, Lane4- Method 4, Lane 5- Kit 1, Lane 6- Kit 2) and DNA ladder (M is 100bp ladder).
Figure 3: TaqMan® probe based real time PCR amplification of 18SSUr RNA gene for method 1 to 4 with No template Control (NTC).
Table 1 . Comparison of different DNA Extraction methods