3.4. TaqMan® probe based Real Time PCR
targeting 18SSU rRNA gene
Further to the confirmation by ”18SSU rRNA nested PCR”, the
goat faecal DNA was also validated with the TaqMan®probe-based Real-Time PCR amplification (Table 3). The results were
encouraging as evidenced by a higher ’RFU’ value and better ’Cq’ value
in the amplification plots. TaqMan® probe-based
Real-Time PCR confirmed the purity and yield of the extracted DNA from
the modified CTAB extraction protocol (Figure 3).
(Figure 3 and Table 3 here)