LEGENDS OF FIGURES
FIGURE 1 Multiple alignment of the deduced amino acid sequence of cpOAS1 protein with that of different species.
FIGURE 2 Phylogram showing the evolutionary relationship among the cDNA sequences of the OAS1 gene of different species by using the MegAlign software. It revealed three distinct clades, clade A includes large ruminant species, clade B includes small ruminant species and clade C includes non-ruminant species.
FIGURE 3 Hydrophilicity analysis of the cpOAS1 protein by ProtScale indicates many hydrophilic amino acids are present in the protein.
FIGURE 4 Multilocalization of cpOAS1 protein by PSORT II (4a) and DeepLoc-1.0 servers (4b)
FIGURE 5 Combination of buried and exposed amino acid residues, RSA and disorders in the cpOAS1 protein
FIGURE 6 Prediction of mucin-type GalNAc O-glycosylation (6A) and Glycation (6B) sites at amino groups of a lysine residue in the cpOAS1 protein.
FIGURE 7 Prediction of 3-D structure of cpOAS1 protein generated from MODELLER 9.18 (P1), SWISS-MODEL (P2), PHYRE 2 (P3) and GENO3D (P4).
FIGURE 8A Ramachandran plot generated from SWISS-MODEL (P2). It shows 94.6 % residues in the most favoured region and 5.4 % additional allowed regions (%) and none in the disallowed region.
FIGURE 8B Superimposition of SWISS-MODEL (P2) model and porcine (4rwp) generated in UCSF Chimera
FIGURE 9 Prediction of 51 different types of eukaryotic linear motifs and 120 instances in the cpOAS1 protein.
FIGURE 10 Prediction of the interaction of cpOAS1 protein with top ten proteins by STRING 11.0 tool.
FIGURE 11 Relative quantitative PCR analysis of cpOAS1 mRNA in the endometrial tissue of pregnant (P) and cyclic does. Bars indicate mean ± SEM values (n = 6). Different letters indicate significant difference (P < 0.05).
FIGURE 12 Detection of OAS protein by western blotting. Detection of OAS protein on PVDF membrane (A) and relative expression of OAS protein (B) in the endometrial tissue of pregnant and non-pregnant does. M: Molecular weight markers (Himedia); Lanes 1: Non-pregnant doe; Lanes 2: 16 to 24 days pregnant doe; Lane 3: 25 to 40 days pregnant doe. Bars indicate mean ± SEM values (n = 6). Different alphabets indicate significant difference (P < 0.05).