3.1 ǀ Amplification, sequencing and characterization of cpOAS1 gene
A fragment of a single specific and expected 890 bp in length was amplified and sequenced (FIGURE 1S). Accordingly, the nucleotide sequence of the cpOAS1 gene was submitted to GenBank for non-descript Anjori goat with accession number (MN564841). The comparison of nucleotide and amino acid sequences of cpOAS1 with available OAS1 sequences of Capra hircus (predicted), Ovis aries(predicted), Bos taurus (predicted), Bubalus bubalis ,Bison bison (predicted), Bos mutus and Sus scrofaconfirmed the cpOAS1 gene (FIGURE 2S and 1). Complementary DNA (cDNA) sequence of cpOAS1 shared 99.6, 96.1, 94.4, 94.3, 95.2, 94.6, 94.4, 80.6 and 80.4 % with Capra hircus (predicted), Ovis aries(predicted), Bos indicus (predicted), Bos taurus ,Bubalus bubalis , Bison bison (predicted), Bos mutus , Sus scrofa and Equuscabalus , respectively (FIGURE 3S). Deduced amino acid sequence of cpOAS1 shared 99.3, 92.6, 89.9, 89.9, 91.2, 90.2, 88.9, 72.3 and 75.0% identity with Capra hircus (predicted), Ovis aries (predicted), Bos indicus(predicted), Bos taurus , Bubalus bubalis , Bison bison (predicted), Bos mutus , Sus scrofa and Equus cabalus , respectively (FIGURE 4S).