3.4 ǀ Expression of cpOAS1mRNA
Constitutive expression of cpOAS1 mRNA was detected in pregnant and non-pregnant endometrium of does. Relative mRNA transcript abundance of cpOAS1 gene was significantly (P<0.05) higher during pregnancy (days 16 to 40 as compared to cyclic does (FIGURE 11). The mean fold change in cpOAS1 mRNA was 10.34 and 3.96 fold greater (P<0.05) during early and later stages of pregnancy, respectively, as compared to cyclic does (FIGURE 11.). cpOAS1 gene showed a single peak in the dissociation curve indicating specificity of qPCR reaction and it also signified that primer was highly specific to the target and had no primer dimer formation (FIGURE 11S).