2.3.1 ǀ Sequence retrieval and Physico-chemical parameters of cpOAS1
The submitted and published cpOAS1 (Acc No MN564841) template from the present study was used for bioinformatics analysis. This template was blasted against the PDB database using Blast P in NCBI Blast, using default parameters. Monomer porcine (PDB ID: 4rwp) template was selected based on maximum identity among the amino acids, e-value, query coverage and other properties. This template was used for predicting the 3-D structure of the cpOAS1 protein. ProtParam tool of ExPASy, SIB Bioinformatics resource portal (http://web.expasy.org/protparam/), was used to compute the basic Physico-chemical properties of cpOAS1 like molecular weight, theoretical isoelectric point (pI), the total number of positive (Asp + Glu) and negative residues(Arg + Lys), formula, number of atoms, extinction coefficient (EC)28, estimated half-life29, instability index (II)30, aliphatic index (AI;)31 and grand average hydropathy (GRAVY)32. The Kyte-Doolittle scale was used for detecting hydrophobic regions in the cpOAS1 proteins. Regions with positive and negative values are depicted as hydrophobic and hydrophilic, respectively. Under this scale, the window size was kept at 9, the relative weight for window edges was 100 % and the linear weighted variation model was selected. Hydrophobicity was computed by the ProtScale.