2.3.4 ǀ Prediction of post-translational modifications (PTMs) in
cpOAS1
Online prediction servers available at the center for biological
sequence analysis (CBS), Technical University of Denmark
(http://www.cbs.dtu.dk/) were used to predict C-mannosylation
sites (NetCGlyc 1.0 server)39, N-terminal
acetylationsites40 (NetAcet 1.0 server),
glycosylphosphatidylinositol anchoring41(GPI-anchoring
or glypiation;
NetGPI-1.1 server), N-linked glycosylation sites (NetNGlyc1.0 server;
http://www.cbs.dtu.dk/services/NetNGlyc/), mucin-type GalNAc
O-glycosylation sites42 (NetOGlyc - 4.0), Glycation of
amino groups of lysines43 (NetGlycate 1.0 server),
phosphorylation sites44 (both generic and
kinase-specific predictions; NetPhos-3.1 server) and arginine and lysine
propeptide cleavage sites45 (ProP 1.0 server).
Prediction of acetylation on internal lysines (PAIL) of cpOAS1 protein
was done using PAIL software46. The fourth-generation
GPS algorithm integrated with the particle swarm optimization
(PSO)47 method was employed for the prediction of
sumoylation sites in the cpOAS1 protein. Disulfide bridges were
predicted in the cpOAS1 protein sequence using CYS_REC
(http://www.softberry.com/cgi-bin/
programs/propt/cys_rec) tool.