3.1 ǀ Amplification, sequencing and characterization of cpOAS1
gene
A fragment of a single specific and expected 890 bp in length was
amplified and sequenced (FIGURE 1S). Accordingly, the nucleotide
sequence of the cpOAS1 gene was submitted to GenBank for non-descript
Anjori goat with accession number (MN564841). The comparison of
nucleotide and amino acid sequences of cpOAS1 with available OAS1
sequences of Capra hircus (predicted), Ovis aries(predicted), Bos taurus (predicted), Bubalus bubalis ,Bison bison (predicted), Bos mutus and Sus scrofaconfirmed the cpOAS1 gene (FIGURE 2S and 1). Complementary DNA (cDNA)
sequence of cpOAS1 shared 99.6, 96.1, 94.4, 94.3, 95.2, 94.6, 94.4, 80.6
and 80.4 % with Capra hircus (predicted), Ovis aries(predicted), Bos indicus (predicted), Bos taurus ,Bubalus bubalis , Bison bison (predicted), Bos
mutus , Sus scrofa and Equuscabalus , respectively (FIGURE
3S). Deduced amino acid sequence of cpOAS1 shared 99.3, 92.6, 89.9,
89.9, 91.2, 90.2, 88.9, 72.3 and 75.0% identity with Capra
hircus (predicted), Ovis aries (predicted), Bos indicus(predicted), Bos taurus , Bubalus bubalis , Bison
bison (predicted), Bos mutus , Sus scrofa and Equus
cabalus , respectively (FIGURE 4S).