Figure 3. The minigene splicing assays based on the pSPL3 exon
trapping vector.
A. Schematic diagram of the in vitro minigene plasmid
construction. The pSPL3 plasmid contains two exons, SD and SA. The
primers were specify to exon SD and SA, respectively.
B-C. Agarose gel electrophoresis (B) and Sanger sequencing (C) for the
PCR products of c.82-2A and c.82-2A>G mutant. β-actin was
used as loading control.
D-E. Agarose gel electrophoresis (D) and Sanger sequencing (E) for the
PCR products of c.290T and c.290dupT mutant. The c.290dupT was highlight
with grey background.
F. Relative quantification of each segment of PCR product from pSPL3
control, c.290T and c.290dupT.