Figure 3. The minigene splicing assays based on the pSPL3 exon trapping vector.
A. Schematic diagram of the in vitro minigene plasmid construction. The pSPL3 plasmid contains two exons, SD and SA. The primers were specify to exon SD and SA, respectively.
B-C. Agarose gel electrophoresis (B) and Sanger sequencing (C) for the PCR products of c.82-2A and c.82-2A>G mutant. β-actin was used as loading control.
D-E. Agarose gel electrophoresis (D) and Sanger sequencing (E) for the PCR products of c.290T and c.290dupT mutant. The c.290dupT was highlight with grey background.
F. Relative quantification of each segment of PCR product from pSPL3 control, c.290T and c.290dupT.