Cell culture
Immortalized lymphocytes derived from II-1 and healthy controls were
conducted using epstein-barr virus procured from B95-8 cells (Cell Bank
of the Chinese Academy of Science, China) as mentioned before (Lou et
al., 2018). Immortalized B lymphocytes were culture in
Roswell Park Memorial Institute (RPMI) 1640 medium (Sigma-Aldrich)
supplement with 10% fetal bovine serum (Sigma-Aldrich), 1% (v/v)
penicillin-streptomycin (Beyotime Biotechnology) and 0.25 μg/mL
amphotericin B (Beyotime Biotechnology). HEK293T cells (Cell Bank of the
Chinese Academy of Science) were cultured in
Dulbecco’s Modified Eagle Medium (DMEM) medium (Sigma-Aldrich)
supplement with 12% fetal calf serum (Sigma-Aldrich) and 1% (v/v)
penicillin-streptomycin and 0.25 μg/mL amphotericin B. All cells were
cultured in 37℃ incubator with 5% CO2.