2.4 Scanning Electron Microscopy
Scanning electron microscopy (SEM) was used to study cells immobilized on the fibrous matrix. Small pieces of fibrous matrices with cells cut from various locations in the FBB were examined at 20 kV with FEI Quanta 200 SEM. Samples were fixed in 2.5% glutaraldehyde solution at 4 °C overnight, dehydrated with ethanol progressively from 20% to 100% at 20% increment for 30 min at each step, dried with hexamethyldisilazane (HMDS), and spotter-coated with gold particles under argon gas. The samples were scanned, photographed, and analyzed visually for cell morphology.