2.3 Genome draft assembly
Genome size and heterozygosity were measured using Jellyfish v.2.1.4 and GenomeScope v2.0 (Marcais & Kingsford, 2011; Vurture et al., 2017; Xiao et al., 2019); parameters setting were as follow -k 19 -p 2 -m 100000. The error of the nanopore clean reads was first corrected using NextDenovo (https://github.com/Nextomics/NextDenovo), with the seed cutoff set at 28491bp. The genome draft of C. striatipennis was assembled also using NextDenovo. The nanopore assembled genome draft was polished in three runs for error-corrected long reads using the illumina DNA short reads by NextPolish v1.3.1 (Hu J. et al., 2020). After each polish, the completeness and quality of assembled genome draft of C. striatipennis were assessed using BUSCO based on the dipterta_odb10 database. The genome of the best BUSCO evaluation result was selected as genome draft for subsequent analysis (Manni et al., 2021).