2.1 Test organisms
Chironomus striatipennis Kieffer were cultivated and maintained
under optimal conditions in the water ecological restoration Laboratory
of Shanghai Ocean University. Organisms were reared in a glass chamber
containing fine inorganic sediment (<10 mm), under a 16-hour
light to 6-hour dark cycle and constant aeration. The water temperature
was maintained at 25 ± 1 ℃. The yeast extract solution (CAS Number:
8013-01-2 from Macklin) was provided as food every day.