2.3 Genome draft assembly
Genome size and heterozygosity were measured using Jellyfish v.2.1.4 and
GenomeScope v2.0 (Marcais & Kingsford, 2011; Vurture et al., 2017; Xiao
et al., 2019); parameters setting were as follow -k 19 -p 2 -m 100000.
The error of the nanopore clean reads was first corrected using
NextDenovo
(https://github.com/Nextomics/NextDenovo),
with the seed cutoff set at 28491bp. The genome draft of C.
striatipennis was assembled also using NextDenovo. The nanopore
assembled genome draft was polished in three runs for error-corrected
long reads using the illumina DNA short reads by NextPolish v1.3.1 (Hu
J. et al., 2020). After each polish, the completeness and quality of
assembled genome draft of C. striatipennis were assessed using
BUSCO based on the dipterta_odb10 database. The genome of the best
BUSCO evaluation result was selected as genome draft for subsequent
analysis (Manni et al., 2021).