Figure 1
Dose-dependent [35S]-GTPγS binding induced by NFEPP (a) and fentanyl (b) at pH 6.5 and 7.4. [35S]-GTPγS binding is expressed as percent increase in [35S]-GTPγS binding relative to binding in unstimulated samples (n=6 membrane preparations). Data points represent means ± standard error of the mean (SEM) of specific [35S]-GTPγS binding in % of baseline. P < 0.05 for Log EC50 of NFEPP at pH 6.5 vs 7.4, 2-tailed t test.