Figure 6
Inhibition of calcium signals by NFEPP at pH 6.5 in rat sensory neurons. (a) Fura-2 imaging experiments with rat sensory neurons treated with 100 µM NFEPP (green trace, N=259 neurons, 9 recordings from 3 rats) and non-treated (0 µM NFEPP) (black trace, N=256 neurons, 9 recordings from 3 rats) at pH 6.5 and pH 7. (b) Calcium responses in rat sensory neurons treated with 100 µM fentanyl (red trace, N=445 neurons, 18 recordings from 6 rats), treated with 100 µM fentanyl + 100 µM naloxone (blue trace, N=454 neurons, 18 recordings from 6 rats) and non-treated (vehicle control: DMSO) (black trace, N=463 neurons, 18 recordings from 6 rats) at pH 6.5 and pH 7. (c) Summary, delta ratio 340/360 from (a) & (b) at pH 6.5 and pH 7. *P < 0.05 NFEPP at pH 6.5 vs 7.4; 2-tailed t test. Traces represent means ± SEM.