Figure 8
Effects of different pH values on NFEPP- or fentanyl-induced MOR phosphorylation in HEK293 cells. (a) MOR phosphorylation was analyzed with antibodies specifically reacting with phosphorylated residues T370, S375, T376 and T379. Controls were treated with 1 μM DMSO (negative control) or 10 μM DAMGO (positive control). Total phosphorylation signal recorded from residues (T370, S375, T376, T379) when stimulated with 1 μM NFEPP (b) or fentanyl (c) in a pH-dependent manner (N=16). **P < 0.01; One-way ANOVA on ranks (specific sample pairs are not analyzed). Data are means ± SEM.