Figure 8
Effects of different pH values on NFEPP- or fentanyl-induced MOR
phosphorylation in HEK293 cells. (a) MOR phosphorylation was analyzed
with antibodies specifically reacting with phosphorylated residues T370,
S375, T376 and T379. Controls were treated with 1 μM DMSO (negative
control) or 10 μM DAMGO (positive control). Total phosphorylation signal
recorded from residues (T370, S375, T376, T379) when stimulated with 1
μM NFEPP (b) or fentanyl (c) in a pH-dependent manner (N=16). **P
< 0.01; One-way ANOVA on ranks (specific sample pairs are not
analyzed). Data are means ± SEM.