Simulation in clinical dosing
Using PK data of nacubactam (Mallalieu et al., 2020) and aztreonam (Scully et al., 1983),(Kita et al., 1986) in healthy subjects, %f T>MICi and %f T>CT against strains used in this study were calculated in the case that aztreonam (0.5 g, 1 g, 2 g) and nacubactam (0.05 g, 0.15 g, 0.5 g, 1 g, 2 g) were co-administered every 8 hrs. In the simulation case of %f T>CT, aztreonam 1,200, 2,400, and 4,800 mg kg-1 day-1 in mice were calculated as aztreonam 0.5 g q8h, 1 g q8h, and 2 g q8h in human, respectively, because of similar kinetic transition.