PK analysis
Aztreonam/nacubactam was subcutaneously administered to neutropenic
mice. At 5, 15, 30, 60, 120, 240, and 300 min after administration,
blood samples were collected through cardiac puncture under anesthesia
(n = 3 per time point). Immediately after blood sampling, the mice were
euthanised by cervical dislocation. The blood samples were centrifuged
to separate the plasma and stored at -80 °C until analysis. PK
parameters were calculated by compartmental model analysis of free
plasma nacubactam and aztreonam concentrations using Phoenix
WinNonlinTM (ver. 8.0, Certara, NJ, USA).