Figure 5. Long-term detection of RSV-RFP in lung cDC. BALB/c
mice were infected with RSV-RFP and lung single-cell suspensions were
analysed by flow cytometry to identify RSV-RFP positive
(+) cell subsets. (A) A representative plot showing
that all RSV-RFP+ cells were identified as
RSV+ MHC II (I-A/I-E) high expression cells. (B)
Macrophages, (C) B cells and (D) cDC showed expression of RSV-RFP after
infection. RSV+ B cells and cDC showed a biphasic
increase of RSV-RFP at 5 and 21 days post infection (dpi). (E)
RSV+ cDC marker expression show high levels of CD11c,
MHC II and CD103 at 5 and 21 dpi, and co-expression of CD11b only at 21
dpi. (F) Representative plot of RSV-RFP expression in
CD103+ and CD103– cDC. RSV-RFP
expression was restricted to CD103+ cDC. Data are
displayed as mean ± SEM; n= 6-9 per group. Statistical analysis was done
by Kruskal-Wallis test with Dunn’s multiple comparison compared to
baseline (0 dpi), *p<0.05.