Figure 4. Increased expression of MHC Class II (IAIE) on lung APC after RSV infection . BALB/c mice were infected with RSV-RFP and lung single-cell suspension was analysed by flow cytometry to identify MHC II expression on cDC (A), macrophages (B) and B cells (C). MHC II mean fluorescent intensity (MFI) was significantly increased at 21 dpi in cDC and B cells, whereas macrophage MHC II expression increased at 7 dpi and remained elevated at 21 dpi compared to baseline (0 dpi). Data are displayed as mean ± SEM; n= 6-9 per group. Statistical analysis was done by Kruskal-Wallis test with Dunn’s multiple comparison compared to baseline (0 dpi), *p<0.05.