Bronchoalveolar lavage analysis
Euthanized mice were tracheotomised for bronchoalveolar lavage (BAL)
which was used to examine inflammatory cell infiltrate into the lungs
following infection. Briefly, BAL fluid was collected by slowly infusing
and withdrawing a 1 ml aliquot of 0.9% saline from the lung three
times. Cell viability was determined by trypan blue staining and
differential cell counts were assessed with Leishman’s stain
(Sigma-Aldrich, St Louis, Missouri, USA).