Figure 3. Lung tissue leukocyte subtype responses following RSV-RFP infection. BALB/c mice were infected with RSV-RFP and lung single-cell suspension was analysed by flow cytometry to identify (A) cDC, (B) macrophages, (C) B cells, (D) pDC and (E) granulocytes. The proportions of cDC increased after 7 days post infection (dpi), whereas the proportion of macrophages increased only at 21 dpi. B cells show a small transient increase at 7 dpi, returning to baseline value at 21 dpi. There was no variation in proportion of pDC (D) and granulocytes (E) observed over the course of infection compared to non-infected mice. Data are displayed as mean ± SEM; n= 6-9 per group. Statistical analysis was done by Kruskal-Wallis test with Dunn’s multiple comparison compared to baseline (0 dpi), *p<0.05.