Lung tissue leukocyte subtype responses following RSV-RFP infection
A significant increase in the percentage of lung tissue conventional dendritic cells (cDC) was noted on 7 and 21 dpi (Figure 3A), with lung tissue macrophages also significantly increased at 21 dpi (Figure 3B). Lung B cells numbers were significantly increased at 7 dpi. They remained elevated at 21 dpi although though this was not statistically significant (Fig. 3C). No increase in plasmacytoid DC (pDC) or granulocytes numbers were observed in lung tissue at any time point following infection (Figures 3D and 3E). Thus, lung tissue antigen presenting cell (APC) subsets showed the dominant response to RSV in lung tissue at 7 dpi, with cDC and macrophages remaining significantly elevated above baseline at 21 dpi.