Figure 5. Long-term detection of RSV-RFP in lung cDC. BALB/c mice were infected with RSV-RFP and lung single-cell suspensions were analysed by flow cytometry to identify RSV-RFP positive (+) cell subsets. (A) A representative plot showing that all RSV-RFP+ cells were identified as RSV+ MHC II (I-A/I-E) high expression cells. (B) Macrophages, (C) B cells and (D) cDC showed expression of RSV-RFP after infection. RSV+ B cells and cDC showed a biphasic increase of RSV-RFP at 5 and 21 days post infection (dpi). (E) RSV+ cDC marker expression show high levels of CD11c, MHC II and CD103 at 5 and 21 dpi, and co-expression of CD11b only at 21 dpi. (F) Representative plot of RSV-RFP expression in CD103+ and CD103 cDC. RSV-RFP expression was restricted to CD103+ cDC. Data are displayed as mean ± SEM; n= 6-9 per group. Statistical analysis was done by Kruskal-Wallis test with Dunn’s multiple comparison compared to baseline (0 dpi), *p<0.05.