Samples marked as ‘<1 ng/µL were samples where the DNA concentration was too low to measure using the Qubit broad range kit. Green highlighted samples were found to be positive for S. nitida by both qPCR and manual survey; orange highlighted surveys were found to be positive for S. nitida by qPCR but not manual survey; red highlighted samples were found to be positive for S. nitida by manual survey but not by qPCR.