ORIGINAL RESEARCH
Mengdie Yu
The Research of
Trans-Cinnamaldehyde inhibits invasion and migration in adenomyosis
derived cells
Mengdie Yu1, Fengxin Cui1, Yiran
Zhang1, Keke Zhang1, Xin
Wang1, Yinuo Zhang1, Zilu
Wang1, Yang Liu1, Wei
Shi2
Shandong
University of Traditional Chinese Medicine, Jinan City, Shandong,
China.
Affiliated Hospital of Shandong
University of Traditional Chinese Medicine, Jinan City, Shandong,
China.
Correspondence: Wei Shi,
Affiliated Hospital of Shandong University of Traditional Chinese
Medicine, No.16369 Jingshi Road, Jinan, Shandong 250014, China
Tel/ Fax: 0531-68616800 Email:
sw19781214@163.com
Objective: To investigate the efficacy of Trans-Cinnamaldehyde
(TC) in inhibiting the invasion and migration of adenomyosis (AM) and to
explore its mechanism of action.
Design: Original research.
Population or Sample: Human endometrium tissues were obtained
from AM patients who underwent total hysterectomy for AM in the Linyi
Central Hospital from September 2019 to May 2021 with patients 41 years
old and 45 years old.
Method: The effect of TC in AMDC by CCK-8, immunofluorescence,
transwell invasion assay, cell wound scratch assay and western blot
analysis the protein for Rho/ROCK signaling pathway.
Results: AMDC was characterized with strongly positive staining
for Vimentin and almost negative for CK7, VWF, E-cadherin, SMA. CCK-8
for IC50 = 28.93±0.44 μg/mL and IC50 = 27.67±0.72 μg/mL at 48h. The
final concentrations of TC (0, 6, 12 μg/mL) were selected of the cell
cytotoxicity, T-text test group >0.05. With increasing TC
concentration, cytoskeleton fluorescence intensity, cell invasion and
migration ability diminished, and Rho/ROCK-related protein expression
was significantly downregulated.
Conclusions: TC inhibits the invasive migratory ability of AMDC
by regulating the Rho/ROCK signaling pathway to affect cytoskeletal
rearrangement with a dose-dependent, which are involved in the
pathophysiology of adenomyosis.