Cell Wound Scratch Assay
Cells were plated at a density of 1.2 × 105 cells/well onto 6-well plates and cultured at 37 °C in 5% CO2until a monolayer was formed. After 24 h, artificial wound tracks were created in the confluent monolayers with a 1000 mL blue. Cells were then gently washed with 1% UY DMEM f12 to remove debris and further incubated with different concentrations of TC (0, 6, 12 μg/mL) at 1% UY DMEM f12, took photos with Axiovert 40 (Germany, Carl zeiss) for 0, 24 and 48 h. The migration distance was measured and analyzed using Image pro plus 6.0 software.