Discussion
Adenomyosis is a kind of benign disease, but it exhibits many
characteristics that are similar to malignant tumors. Many tumors
signaling pathways also participate in the development of adenomyosis.
Current studies show that Rho/ROCK signaling pathways consisting of
RhoA, RhoB, RhoC, ROCK1 ROCK2 induces a specific type of cytoskeleton
and migration of tumor cells21. However, the possible
role(s) of Rho/ROCK signaling pathways in adenomyosis remain unclear. In
the present study, the effects of regulating cytoskeleton-induced
invasion and migration may relate to Rho/ROCK signaling pathway on human
endometrial cells and underlying mechanisms were investigated.
At present, there are certain results about the regulation of
cytoskeleton-induced invasion and migration in malignant tumors. For
example, nasopharyngeal carcinoma migration and invasion via
cytoskeletal remodeling22. Reorganization of the
cytoskeleton, cell migration and invasion contribute to colorectal
cancer23. Cytoskeleton reorganization acquires
the migration and invasion potential typical of mesenchymal cells with
the Role in Cancer and Fibrotic Diseases24. Tumor
transcription factors could suppress the expression of epithelial cell
markers (E-cadherin, CK7, Von Willebrand Factor and alpha smooth muscle
Actin) and maintain the phenotype of mesenchymal cells that express
Vimentin 25-27. TC is the main active constituent of
Guizhi serves as a tumor suppressor such as
cutaneous28, cervical29, colorectal
ovarian30-32, bladder33, and breast
cancers34, 35. What’s more, Rho/ROCK signaling pathway
serves as an oncogene in cancer, has inhibitory effects on the
cytoskeleton, migration and invasion of tumor cells, such as colorectal
cancer36. In this study, we found that the AMDC
purified by AM with positive Vimentin and negative E-cadherin, CK7, Von
Willebrand Factor and alpha smooth muscle Actin and our results suggest
that downregulation of cytoskeleton, migration, invasion and may relate
to Rho/ROCK (RhoA, RhoB, RhoC, ROCK1, ROCK2) signaling pathway treating
with TC may also be involved in the pathogenesis of adenomyosis.