Cell Wound Scratch Assay
Cells were plated at a density of 1.2 × 105 cells/well
onto 6-well plates and cultured at 37 °C in 5% CO2until a monolayer was formed. After 24 h, artificial wound tracks were
created in the confluent monolayers with a 1000 mL blue. Cells were then
gently washed with 1% UY DMEM f12 to remove debris and further
incubated with different concentrations of TC (0, 6, 12 μg/mL) at 1% UY
DMEM f12, took photos with Axiovert 40 (Germany, Carl zeiss) for 0, 24
and 48 h. The migration distance was measured and analyzed using Image
pro plus 6.0 software.