Discussion

Adenomyosis is a kind of benign disease, but it exhibits many characteristics that are similar to malignant tumors. Many tumors signaling pathways also participate in the development of adenomyosis. Current studies show that Rho/ROCK signaling pathways consisting of RhoA, RhoB, RhoC, ROCK1 ROCK2 induces a specific type of cytoskeleton and migration of tumor cells21. However, the possible role(s) of Rho/ROCK signaling pathways in adenomyosis remain unclear. In the present study, the effects of regulating cytoskeleton-induced invasion and migration may relate to Rho/ROCK signaling pathway on human endometrial cells and underlying mechanisms were investigated.
At present, there are certain results about the regulation of cytoskeleton-induced invasion and migration in malignant tumors. For example, nasopharyngeal carcinoma migration and invasion via cytoskeletal remodeling22. Reorganization of the cytoskeleton, cell migration and invasion contribute to colorectal cancer23. Cytoskeleton reorganization acquires the migration and invasion potential typical of mesenchymal cells with the Role in Cancer and Fibrotic Diseases24. Tumor transcription factors could suppress the expression of epithelial cell markers (E-cadherin, CK7, Von Willebrand Factor and alpha smooth muscle Actin) and maintain the phenotype of mesenchymal cells that express Vimentin 25-27. TC is the main active constituent of Guizhi serves as a tumor suppressor such as cutaneous28, cervical29, colorectal ovarian30-32, bladder33, and breast cancers34, 35. What’s more, Rho/ROCK signaling pathway serves as an oncogene in cancer, has inhibitory effects on the cytoskeleton, migration and invasion of tumor cells, such as colorectal cancer36. In this study, we found that the AMDC purified by AM with positive Vimentin and negative E-cadherin, CK7, Von Willebrand Factor and alpha smooth muscle Actin and our results suggest that downregulation of cytoskeleton, migration, invasion and may relate to Rho/ROCK (RhoA, RhoB, RhoC, ROCK1, ROCK2) signaling pathway treating with TC may also be involved in the pathogenesis of adenomyosis.