Cell culture conditions for fatty acid biosynthesis
Cells carrying different constructs were incubated in 5 mL of LB medium
supplemented with antibiotics and cultured overnight at 37 °C. Overnight
cell culture (3% [v/v]) was added to a 250 mL flask containing 50
mL of LB medium supplemented with 15 g•L−1 glucose and then cultivated
at 37 °C at a frequency of 150 rpm. Antibiotics were added to maintain
the plasmids in the recombinant strains: ampicillin (100 mg/L),
kanamycin (50 mg/L), or chloramphenicol (12.5 mg/L). The cultures were
induced by adding 0.2% arabinose at OD600 = 0.6, and the samples were
collected at 20 h post-induction for fatty acid analysis.