Figure 2 KitW-sh/W-sh mice showed no
inflammatory reaction induced by tacrolimus
KitW-sh/W-shmice were treated with tacrolimus on dorsal skin for one day. Clinical
photographs (A) and Haematoxylin and eosin (H&E) staining(B) of skin lesions. Scale bar, 50 μm. (C) Scratching
bouts in 20 min and levels of IgE,tryptase, TNF-α and MCP-1 in serum
(mean ± SEM, n = 6). (D) In hind paw swelling and extravasation
experiment, typical hind paw photos, paw thickness increase rate and
OD620nm per g weight of KitW-sh/W-shmice and WT mice (mean ± SEM, n = 6). All experiments were repeated 3
times. Statistical significance was accepted at p < 0.05
(*p < 0.05, **p < 0.01 and ***p<0.005) by
two-tailed unpaired Student’s t-test.