Figure legends
Figure 1 Transient inflammatory reaction and non-IgE related itching were triggered by short-term tacrolimus application in vivo. WT mice were applied with different concentrations of tacrolimus for one day. Clinical photographs(A) and Haematoxylin and eosin (H&E) staining (B) of skin lesions. Scale bar, 50 μm. (C) Immunofluorescence staining of avidin in WT mice. Scale bar, 20 μm. Red arrow points to one of the undegranulated MCs, white arrows point to degranulated MCs. (D)Scratching bouts in 20 min and level of serum IgE (mean ± SEM, n = 6).(E) Levels of tryptase, TNF-α and MCP-1 in serum (mean ± SEM, n = 6). In hind paw swelling and extravasation experiment, (F)typical hind paw photos of WT mice after 15-minutes injection with tacrolimus and C48/80. (G) Paw thickness increase rate and OD620nm per g weight of WT mice (mean ± SEM, n = 6). All experiments were repeated 3 times. Statistical significance was accepted at p < 0.05 (*p < 0.05, **p < 0.01 and ***p<0.005) by two-tailed paired Student’s t-test.