Figure 7 Inflammatory reaction elicited by MrgprB2 could be
suppressed by long-term tacrolimus treatment.
WT mice were treated with tacrolimus and vehicle on dorsal skin
respectively for 11 d. C48/80 was subcutaneously injected after 7- and
11- day exposure to tacrolimus. (A) Scratching bouts in 20 min
on day 7 and 11 respectively (mean ± SEM, n = 6). (B) H&E
staining of skin lesions. Scale bar, 50 μm. And inflammatory cell
infiltration per field of H&E image on day 7 and 11 respectively (mean
± SEM, n = 6). (C) Level of skin IgE (mean ± SEM, n = 6).(D) Immunofluorescence staining of avidin. Scale bar,5 μm. Red
arrow points to one of the undegranulated MCs, white arrows point to
degranulated MCs. MC infiltration per field, degranulation per field and
the degranulation rate were counted in avidin staining images on day 7
and 11 respectively (mean ± SEM, n = 6). (E) Levels of skin
tryptase, IL-8, TNF-α and MCP-1 on day 7 and 11 respectively (mean ±
SEM, n = 6). (F) Skin tryptase, IL-8, TNF-α and MCP-1 mRNA
expression on day 7 and 11 respectively (mean ± SEM, n = 6). All
experiments were repeated 3 times. Statistical significance was accepted
at p < 0.05 (*p < 0.05, **p < 0.01
and ***p<0.005) by two-tailed unpaired Student’s t-test.