Figure 8 MCs-mediated inflammation could be inhibited by long-term tacrolimus treatment via suppression of MRGPRX2 expression.
Human LAD2 cells were treated with 0.5 nM tacrolimus and vehicle respectively for 3 d. (A) MRGPRX2 relative mRNA expression in LAD2 cells at day 1-3 respectively (mean ± SEM, n = 3). (B)β-hexosaminidase release rate of LAD2 cells activation by C48/80 at day 1-3 respectively (mean ± SEM, n = 3). (C) Western blot analysis of the expression levels of MRGPRX2 and β-actin in LAD2 cells on day 3, and quantification of MRGPRX2 protein expression respectively by densitometric analysis. (D) Different concentrations of C48/80 induced LAD2 release tryptase, TNF-α, IL-8 and MCP-1 on day 3 (mean ± SEM, n = 3). All experiments were repeated 3 times. Statistical significance was accepted at p < 0.05 (*p < 0.05, **p < 0.01 and ***p<0.005) by two-tailed unpaired Student’s t-test.