Figure 6 Initial inflammation induced by tacrolimus faded away
and MrgprB2 expression was suppressed after tacrolimus long-term use.
WT mice were applied with and vehicle on dorsal skin tacrolimus for 7
days and 11 days respectively. (A) Scratching bouts in 20 min
on day 7 and 11 respectively (mean ± SEM, n = 6). (B) H&E
staining of skin lesions. Scale bar, 50 μm. And inflammatory cell
infiltration per field of H&E image on day 7 and 11 respectively (mean
± SEM, n = 6). (C) Level of skin IgE on day 7 and 11
respectively (mean ± SEM, n = 4). (D) Levels of skin TNF-α,
MCP-1, IL-8 and tryptase on day 7 and 11 respectively (mean ± SEM, n =
6). (E) Immunofluorescence staining of avidin on day 7 and 11.
Scale bar, 5 μm. MC infiltration per field, degranulation per field and
the degranulation rate were counted in avidin staining images on day 7
and 11 respectively (mean ± SEM, n = 6). (F) Skin MrgprB2
relative mRNA expression on day 7 and 11 respectively (mean ± SEM, n =
6). All experiments were repeated 3 times. Statistical significance was
accepted at p < 0.05 (*p < 0.05, **p
< 0.01 and ***p<0.005) by two-tailed unpaired Student’s
t-test.