Statistical analysis
All the experiments were repeated at least 3 times. Data are expressed
as mean ± SEM and analyzed using two-tailed unpaired student’s t-test.
An independent samples analysis of variance was used to determine
statistical significance in comparisons of the data using the SPSS
software. Differences were considered significant at *P< 0.05, **P < 0.01, ***P <
0.005.
Details about other methods including reagents, scratching bouts,
histological analysis, β-hexosaminidase assay, intracellular calcium
mobilization assay, ELISA, western blot and real time qPCR analysis are
provided in the Data S1