Figure 3 Immediate effect of tacrolimus primed MrgprB2-elicited
MCs activation in vivo.
In hind paw swelling and extravasation experiment, (A) typical
hind paw photos, paw thickness increase rate and OD620nmper g weight of MUT mice and WT mice (mean ± SEM, n = 6). MUT mice were
treated with tacrolimus on dorsal skin for one day. Clinical photographs(B) and Haematoxylin and eosin (H&E) staining (C) of
skin lesions. Scale bar, 50 μm. (D) Immunofluorescence staining
of avidin in MUT mice after 1-d tacrolimus application. Scale bar, 20
μm. Red arrow points to one of the undegranulated MCs, white arrows
point to degranulated MCs. (E) Scratching bouts in 20 min and
levels of IgE,tryptase, TNF-α and MCP-1 in serum (mean ± SEM, n = 6).
All experiments were repeated 3 times. Statistical significance was
accepted at p < 0.05 (*p < 0.05, **p
< 0.01 and ***p<0.005) by two-tailed unpaired Student’s
t-test.