Hind paw swelling and extravasation
The experiment was performed as described
previously19. Briefly, male C57BL/6 mice, MUT and
KitW-sh/W-sh mice were anesthetized with
pelltobarbitalum natricum. Fifteen minutes after the induction of
anesthesia, each mouse was injected intravenously (i.v.) with 0.2 ml
Evans blue dye in saline. Then, 5 μl of tacrolimus (31.25, 62.5, 125 μM)
or C48/80 (30 μg/ml) was administered into the left paw, saline into the
right paw as a blank control. Fifteen minutes later, the paw thickness
was measured again and mice were sacrificed for further analysis.