Figure 3 Immediate effect of tacrolimus primed MrgprB2-elicited MCs activation in vivo.
In hind paw swelling and extravasation experiment, (A) typical hind paw photos, paw thickness increase rate and OD620nmper g weight of MUT mice and WT mice (mean ± SEM, n = 6). MUT mice were treated with tacrolimus on dorsal skin for one day. Clinical photographs(B) and Haematoxylin and eosin (H&E) staining (C) of skin lesions. Scale bar, 50 μm. (D) Immunofluorescence staining of avidin in MUT mice after 1-d tacrolimus application. Scale bar, 20 μm. Red arrow points to one of the undegranulated MCs, white arrows point to degranulated MCs. (E) Scratching bouts in 20 min and levels of IgE,tryptase, TNF-α and MCP-1 in serum (mean ± SEM, n = 6). All experiments were repeated 3 times. Statistical significance was accepted at p < 0.05 (*p < 0.05, **p < 0.01 and ***p<0.005) by two-tailed unpaired Student’s t-test.