Figure 7 Inflammatory reaction elicited by MrgprB2 could be suppressed by long-term tacrolimus treatment.
WT mice were treated with tacrolimus and vehicle on dorsal skin respectively for 11 d. C48/80 was subcutaneously injected after 7- and 11- day exposure to tacrolimus. (A) Scratching bouts in 20 min on day 7 and 11 respectively (mean ± SEM, n = 6). (B) H&E staining of skin lesions. Scale bar, 50 μm. And inflammatory cell infiltration per field of H&E image on day 7 and 11 respectively (mean ± SEM, n = 6). (C) Level of skin IgE (mean ± SEM, n = 6).(D) Immunofluorescence staining of avidin. Scale bar,5 μm. Red arrow points to one of the undegranulated MCs, white arrows point to degranulated MCs. MC infiltration per field, degranulation per field and the degranulation rate were counted in avidin staining images on day 7 and 11 respectively (mean ± SEM, n = 6). (E) Levels of skin tryptase, IL-8, TNF-α and MCP-1 on day 7 and 11 respectively (mean ± SEM, n = 6). (F) Skin tryptase, IL-8, TNF-α and MCP-1 mRNA expression on day 7 and 11 respectively (mean ± SEM, n = 6). All experiments were repeated 3 times. Statistical significance was accepted at p < 0.05 (*p < 0.05, **p < 0.01 and ***p<0.005) by two-tailed unpaired Student’s t-test.