Figure legends
Figure 1 Transient inflammatory reaction and non-IgE related
itching were triggered by short-term tacrolimus application in
vivo. WT mice were applied with
different concentrations of tacrolimus for one day. Clinical photographs(A) and Haematoxylin and eosin (H&E) staining (B) of
skin lesions. Scale bar, 50 μm. (C) Immunofluorescence staining
of avidin in WT mice. Scale bar, 20 μm. Red arrow points to one of the
undegranulated MCs, white arrows point to degranulated MCs. (D)Scratching bouts in 20 min and level of serum IgE (mean ± SEM, n = 6).(E) Levels of tryptase, TNF-α and MCP-1 in serum (mean ± SEM, n
= 6). In hind paw swelling and extravasation experiment, (F)typical hind paw photos of WT mice after 15-minutes injection with
tacrolimus and C48/80. (G) Paw thickness increase rate and
OD620nm per g weight of WT mice (mean ± SEM, n = 6). All
experiments were repeated 3 times. Statistical significance was accepted
at p < 0.05 (*p < 0.05, **p < 0.01
and ***p<0.005) by two-tailed paired Student’s t-test.