Comparison of MiSeq barcodes with Sanger barcodes
Full-length barcodes were successfully recovered by both MiSeq and Sanger barcoding approaches for 68 of 96 specimens subjected to both methods. Full length Illumina barcodes were obtained from seven specimens, and partial Illumina barcodes (one LCO-only and eight BR-only) from a further nine specimens, that each failed to produce Sanger barcodes; 15 of these 16 specimens were beetles. On the other hand, only partial barcodes (one LCO-only and nine BR-only) were recovered using the Illumina method from ten specimens from which full-length Sanger barcodes were obtained. No barcodes were obtained using either method from just two specimens (Ephutomorpha bivulnerata , a wasp; and the single mite specimen).
Among 68 specimens from which both Sanger and Illumina barcodes were recovered, pairwise sequence identities between these barcodes were from 75.5 % to 85.7 % for three specimens, 97.6 % to 98.6 % for six specimens, 99.1 % to 99.9 % for 18 specimens, and 100 % for 40 specimens.