Conclusion
As the need for DNA-based biodiversity assessment continues to grow [12, 51], the development of fit-for-purpose and reliable reference databases follows. Further, the development of methods for non-destructive DNA extraction from museum/stored samples has created an opportunity to develop rapid barcoding technology. Along with recent attempts at using Illumina and PacBio technologies [12, 23], we provide another approach for rapid and high-throughput barcode generation. Our taxonomy-informed pipeline leverages the benefits and value of keeping specimens in taxonomic collections in the long-term and helps develop targeted reference databases to support regional and national biodiversity surveys.