Conclusion
As the need for DNA-based biodiversity assessment continues to grow
[12, 51], the development of fit-for-purpose and reliable reference
databases follows. Further, the development of methods for
non-destructive DNA extraction from museum/stored samples has created an
opportunity to develop rapid barcoding technology. Along with recent
attempts at using Illumina and PacBio technologies [12, 23], we
provide another approach for rapid and high-throughput barcode
generation. Our taxonomy-informed pipeline leverages the benefits and
value of keeping specimens in taxonomic collections in the long-term and
helps develop targeted reference databases to support regional and
national biodiversity surveys.